期刊
ANTIOXIDANTS
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/antiox10091422
关键词
follicle atresia; cecal microbiota; metabolomics; melatonin; SIRT1-P53; FoxO1 pathway; ovary stress biomarker
资金
- National Natural Science Foundation of China [31872792]
- Sichuan Science and Technology Program [2019YFH0062, 2018NZ20009]
The study using the poultry ovary model found that oxidative stress can lead to reduced reproductive performance, impaired ovarian function, and altered gut microbiota. Melatonin can ameliorate ovary oxidative stress through the SIRT1-P53/FoxO1 pathway.
The poultry ovary is used as a classic model to study ovarian biology and ovarian cancer. Stress factors induced oxidative stress to cause follicle atresia, which may be a fundamental reason for the reduction in fertility in older laying hens or in aging women. In the present study, we set out to characterize the relationships between oxidative stress and ovarian function. Layers (62 weeks of age; BW = 1.42 +/- 0.12 kg) were injected with tert-butyl hydroperoxide (tBHP) at 0 (CON) and 800 mu mol/kg BW (oxidative stress group, OS) for 24 days and the role of melatonin (Mel) on tBHP-induced ovary oxidative stress was assessed through ovary culture in vitro. The OS (800 mu mol/kg BW tert-butyl hydroperoxide) treatment decreased the reproduction performance and ovarian follicle numbers. OS decreased the expression of SIRT1 and increased the P53 and FoxO1 expression of the ovary. A decreased Firmicutes to Bacteroidetes ratio, enriched Marinifilaceae (family), Odoribacter (genus) and Bacteroides_plebeius (species) were observed in the cecum of the OS group. Using Mel in vitro enhanced the follicle numbers and decreased the ovary cell apoptosis induced by tBHP. In addition, it increased the expression of SIRT1 and decreased the P53 and FoxO1 expression. These findings indicated that oxidative stress could decrease the laying performance, ovarian function and influence gut microbiota and body metabolites in the layer model, while the melatonin exerts an amelioration the ovary oxidative stress through SIRT1-P53/FoxO1 pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据