期刊
ANTIOXIDANTS
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/antiox10081298
关键词
atherosclerosis; low-density lipoprotein (LDL); oxidized LDL; malondialdehyde; anti-oxidized LDL antibodies; antibodies; assay; inflammation
资金
- BHF Clinical Research Fellowship
- Wellcome Trust Clinical Research Fellowship, Wellcome Trust/GSK Fellowship programme [095034/Z/10/Z]
- Wellcome Trust Clinical Research Fellowship [220572/Z/20/Z]
- National Institute for Health Research (NIHR) Biomedical Research Centre (BRC) at Imperial College London
- Raissa and Anton Sansour Fund at Imperial Healthcare Charity
- British Heart Foundation (BHF)
- Wellcome Trust [095034/Z/10/Z, 220572/Z/20/Z] Funding Source: Wellcome Trust
This study introduces a sensitive novel enzyme-linked immunosorbent assay for the detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of accurately measuring MDA-LDL levels in human plasma and serum, with high inter-operator reproducibility.
Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r = 0.74, p < 0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable translational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability.
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