Article
Biochemistry & Molecular Biology
Maryam Golshani, Waheed Ur Rahman, Adriana Osickova, Jana Holubova, Jinery Lora, Nataliya Balashova, Peter Sebo, Radim Osicka
Summary: The pertussis agent Bordetella pertussis produces a virulence factor called filamentous hemagglutinin (FhaB) that plays a crucial role in adhesion and infection. FhaB protein translocates through an FhaC pore to the outer bacterial surface and is then processed to a smaller fragment. Contrary to previous suggestions, the study found that FhaB does not bind the beta(2) integrin CD11b/CD18 but instead interacts with sulfated glycosaminoglycans on the cell surface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Drusilla L. Burns
Summary: Bordetella pertussis relies on a two-step secretion process to produce and secrete pertussis toxin, involving transport across both the inner and outer membranes of the bacterial cell. The specialized Ptl transporter is essential for the outer membrane transport of PT, distinguishing it from canonical type IV substrate transport.
Article
Immunology
Karen M. Scanlon, Ling Chen, Nicholas H. Carbonetti
Summary: The study revealed that Bordetella pertussis-induced pulmonary hypertension is age-related and depends on the expression of pertussis toxin by the bacterium. Targeting pertussis toxin in treatment may help alleviate pulmonary hypertension and fatal infant infections.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Jingjing Gao, Linlin Huang, Shuquan Luo, Ruijie Qiao, Fanglei Liu, Xin Li
Summary: In this study, a two-component pertussis vaccine candidate was developed with the ability to induce a mixed Th1/Th2/Th17 immune response and demonstrate strong bactericidal activity and IgG response. The vaccine also showed efficient prophylactic effects against B. pertussis in a mouse model. Therefore, it has the potential to be the next generation of pertussis vaccines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Yukihiro Hiramatsu, Koichiro Suzuki, Takashi Nishida, Naoki Onoda, Takashi Satoh, Shizuo Akira, Masahito Ikawa, Hiroko Ikeda, Junzo Kamei, Sandra Derouiche, Makoto Tominaga, Yasuhiko Horiguchi
Summary: The Gram-negative bacterium Bordetella pertussis causes whooping cough, a respiratory disease characterized by paroxysmal coughing. However, the mechanism of coughing has not been extensively studied. In this study, a mouse model was used to reproduce coughing after intranasal inoculation with the bacterium. It was found that lipooligosaccharide, Vag8, and pertussis toxin of the bacteria cooperatively function to cause coughing. Bradykinin and TRPV1 were identified as host factors involved in the coughing mechanism.
Review
Food Science & Technology
Camille Locht
Summary: This article summarizes the main contributions of scientists from Pasteur Institutes to the current knowledge of toxins produced by Bordetella pertussis. It highlights the identification of B. pertussis as the causative agent of whooping cough and the significant contributions made by Pasteurians in understanding the structure-function relationship of key toxins. It also discusses the potential applications of this knowledge in various fields, including protein-protein interaction studies, antigen delivery tools, and vaccine development.
Article
Immunology
Colleen J. Sedney, Amanda Caulfield, Kaylan K. Dewan, Uriel Blas-Machado, Maiya Callender, Nancy R. Manley, Eric T. Harvill
Summary: The increased susceptibility of neonates to specific pathogens has previously been attributed to an underdeveloped immune system. More recent data suggest neonates have effective protection against most pathogens but are particularly susceptible to those that target immune functions specific to neonates. Bordetella pertussis (Bp), the causative agent of whooping cough, causes more serious disease in infants attributed to its production of pertussis toxin (PTx), although the neonate-specific immune functions it targets remain unknown. The study found that neonatal mice from five to eight days old are more susceptible to Bp than slightly older mice, and they can effectively respond to and control Bp lacking PTx. PTx efficiently blocks some very effective form(s) of neonatal protective immunity, potentially providing a tool to better understand the neonatal immune system. The results also demonstrate a striking age-dependent response to Bp and define an early age of extreme susceptibility to Bp. The study's findings may be useful in studying other pathogens that primarily infect neonates and PTx may prove a particularly valuable tool for probing the poorly understood neonatal immune system.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhen Wang, Fengfeng Fan, Jianli Wang, Liangjia Wang, Hao Hu, Chenhui Wang, Xiaoyuan Wang
Summary: The immunogenicity of pertussis vaccine can be enhanced by adding Bordetella pertussis oligosaccharide with multiple trisaccharide units. This study engineered Escherichia coli to produce B. pertussis oligosaccharides with multiple trisaccharide units, leading to improved immune response efficiency.
METABOLIC ENGINEERING
(2022)
Article
Immunology
Soraya Matczak, Valerie Bouchez, Pauline Leroux, Thibaut Douche, Nils Collinet, Annie Landier, Quentin Giai Gianetto, Sophie Guillot, Julia Chamot-Rooke, Milena Hasan, Mariette Matondo, Sylvain Brisse, Julie Toubiana
Summary: By comparing the microbiological characteristics and expressed protein profiles between FIM2 and FIM3 fimbrial serotypes and genomic clades, differences were found that may have implications on pathogenesis and epidemiological emergence.
MICROBES AND INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Negar Souod, Elham Rismani, Fariborz Bahrami, Saeed Reza Pakzad, Soheila Ajdary
Summary: This study designed a novel chimeric protein using C-CPE as a delivery system and F1S1 fragment of B. pertussis to target Claudin-4 receptors in mice lung cells. Molecular dynamic simulation showed the fusion protein had a stable structure, suggesting its potential as a vaccine candidate against B. pertussis infection.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Food Science & Technology
Julie Tomas, Yoon Koo, Dimitri Popoff, Vilma Arce-Gorvel, Sean Hanniffy, Jean-Pierre Gorvel, Cyrille Mionnet
Summary: Whooping cough caused by Bordetella pertussis is a severe and highly contagious disease of the human respiratory tract. The pathogenicity requires several virulence factors, including pertussis toxin (PTX). Current vaccines do not induce mucosal immunity, but research shows that PTX is critical for generating lung CD4(+) and CD8(+) Trm. Adoptive transfer of these Trm subsets can confer protection against B. pertussis in naive mice.
Article
Biochemistry & Molecular Biology
Anna Malandra, Waheed Ur Rahman, Nela Klimova, Gaia Streparola, Jana Holubova, Adriana Osickova, Simone Bariselli, Peter Sebo, Radim Osicka
Summary: Research shows that Bordetella pertussis toxins CyaA and PT can induce the production of major airway mucins in infected airway epithelial cells by activating the cAMP response element binding protein (CREB). Mutant strains of B. pertussis that can only secrete active PT or produce enzymatically inactive CyaA-AC(-) toxoid fail to trigger significant mucus production, while strains secreting active CyaA toxin and PT toxoid can elicit mucin production efficiently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Jinfang Jia, Maria Braune-Yan, Stefanie Lietz, Mary Wahba, Arto T. Pulliainen, Holger Barth, Katharina Ernst
Summary: Bordetella pertussis toxin (PT) and Clostridium botulinum C2 toxin are ADP-ribosylating toxins that require cellular chaperones Hsp90 and Hsp70, cyclophilins, and FK506-binding proteins for translocation. Inhibition of chaperone activities can protect cells from PT and C2 intoxication. Domperidone, a dopamine receptor antagonist, was found to inhibit Hsp70 activity and protect cells from intoxication. Domperidone inhibits enzyme subunit translocation and reduces toxin uptake, making it a potential therapeutic agent for diseases caused by bacterial toxins that require Hsp70.
Article
Food Science & Technology
Lisa Szymkowicz, Jeffery Gerard, Benjamin Messham, Wai Wan Vivian Tam, D. Andrew James
Summary: This paper describes an LC-MS method for simultaneous analysis of residual protein toxins and small molecule glycopeptides in Pertussis toxin vaccine antigens. While ACT and TCT are quantified using MRM, DNT is currently monitored through animal testing, and new approaches for DNT testing are discussed.
Article
Immunology
Megan A. DeJong, M. Allison Wolf, Graham J. Bitzer, Jesse M. Hall, Emel Sen-Kilic, Jeanna M. Blake, Jonathan E. Petty, Ting Y. Wong, Mariette Barbier, John D. Campbell, Justin R. Bevere, F. Heath Damron
Summary: The study evaluates the potential of CpG 1018 adjuvant to improve the immune response and protection against B. pertussis. The results show that adding CpG 1018 to the Tdap vaccine enhances antibody responses and bacterial clearance.
Article
Biotechnology & Applied Microbiology
Xin Li, Chao Pan, Zhicheng Liu, Peng Sun, Xiaoting Hua, Erling Feng, Yunsong Yu, Jun Wu, Li Zhu, Hengliang Wang
Summary: This study developed a conjugate vaccine against A. baumannii, demonstrating its ability to induce immune responses and provide protection against infection, ultimately proving its promising potential as a candidate vaccine.
MICROBIAL BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Chao Pan, Hua Yue, Li Zhu, Guang-hui Ma, Heng-liang Wang
Summary: Prophylactic vaccines have evolved from traditional whole-cell vaccines to safer subunit vaccines, but face challenges such as poor immunogenicity. Advanced delivery vectors play a crucial role in overcoming these challenges, offering efficient and safe immune responses. Summarizing and comparing delivery systems for infectious pathogens can provide insights for future vaccine development.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Physical
Xin Li, Chao Pan, Peng Sun, Zhehui Peng, Erling Feng, Jun Wu, Hengliang Wang, Li Zhu
Summary: The research utilized the SpyTag/SpyCatcher system to produce nanoconjugate vaccines, demonstrating efficient prophylactic effects by conjugating polysaccharides onto the surfaces of virus-like particles.
Article
Chemistry, Multidisciplinary
Zhehui Peng, Jun Wu, Kangfeng Wang, Xin Li, Peng Sun, Lulu Zhang, Jing Huang, Yan Liu, Xiaoting Hua, Yunsong Yu, Chao Pan, Hengliang Wang, Li Zhu
Summary: A nanoscale conjugate vaccine against Klebsiella pneumoniae was successfully produced using a general Escherichia coli host system. The vaccine induced an efficient humoral immune response in draining lymph nodes and high titers of IgG antibody against bacterial lipopolysaccharide, demonstrating ideal prophylactic effects in mouse models of systemic and pulmonary infection. This study opens up possibilities for developing vaccines against other pathogenic bacteria using similar strategies.
Review
Immunology
Wenping Gong, Chao Pan, Peng Cheng, Jie Wang, Guangyu Zhao, Xueqiong Wu
Summary: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The COVID-19 pandemic has led to an increase in global TB mortality rate, necessitating the development of more effective TB vaccines. Peptides have been found to play important roles in immune response. This review provides an overview of tools for designing peptide-based vaccines, the current research status of such vaccines for TB, protein-based bacterial vaccine delivery systems, and animal models for peptide-based vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Li Liu, Pengju Wang, Dongdong Zhao, Li Zhu, Jinlei Tang, Wenchuan Leng, Junchang Su, Yan Liu, Changhao Bi, Xueli Zhang
Summary: Biomaterials, such as spider silk, have unique properties that make them highly valuable for next-generation applications. A new technique using circularized mRNA (cmRNA) has been developed to simplify the construction and expression of fibrous proteins, allowing for the production of large amounts of spider silk proteins with high efficiency and authenticity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yixin Shi, Chao Pan, Kangfeng Wang, Yan Liu, Yange Sun, Yan Guo, Peng Sun, Jun Wu, Ying Lu, Li Zhu, Hengliang Wang
Summary: Researchers have developed a proteinaceous nanoparticle chassis that can combine with various antigens to create high-performance nanovaccines. By appending a sugar chain on the surface of the nanoparticles, they were able to increase the yield and demonstrate the ability to induce immune responses. This study provides a new technology for preparing nanoscale vaccines.
Article
Microbiology
Yufei Lyu, Dongshu Wang, Lu Yuan, Erling Feng, Li Zhu, Chao Pan, Yan Guo, Xiankai Liu, Hengliang Wang
Summary: Bacillus anthracis is a dangerous bacterium that causes life-threatening infections and is used as a bioterror agent. This study identifies eight new SNP sites for the specific identification of B. anthracis and develops software and methods for rapid and reliable detection. The findings have important implications for public health responses to disease outbreaks and bioterrorism threats.
MICROBIOLOGY SPECTRUM
(2022)
Article
Health Care Sciences & Services
Mengting Mo, Xiang Li, Caixia Li, Kangfeng Wang, Shulei Li, Yan Guo, Peng Sun, Jun Wu, Ying Lu, Chao Pan, Hengliang Wang
Summary: Researchers discovered that covalently coupling CpG with a nanovaccine can significantly enhance the utilization of CpG in lymph nodes and promote cellular immune response. This approach is simple, convenient, and particularly suitable for enhancing immune effect of vaccines against intracellular bacteria.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Xiang Li, Chao Pan, Caixia Li, Kangfeng Wang, Jingqin Ye, Peng Sun, Yan Guo, Jun Wu, Hengliang Wang, Li Zhu
Summary: Nanotechnology has led to the development of nanovaccinology, with protein-based nanocarriers gaining attention for their biocompatibility. This study designed a multifunctional nanocarrier capable of delivering various biomolecules by fusing the cholera toxin B subunit with streptavidin. The nanocarrier was used to prepare a bioconjugate nanovaccine against S. flexneri, stimulating both adaptive and innate immunity and improving the survival of vaccinated mice. The design strategy demonstrated in this study could be applied in the development of nanovaccines against infectious diseases.
ACS APPLIED NANO MATERIALS
(2023)
Article
Immunology
Peng Sun, Xin Li, Chao Pan, Zhicheng Liu, Jun Wu, Hengliang Wang, Li Zhu
Summary: This study demonstrates that the trimeric autotransporter protein Ata(α) of Acinetobacter baumannii is a promising vaccine target for A. baumannii infections. The fusion protein CTB-Ata(α) induces immune responses, reduces bacterial loads, and provides broad protection in mice.
FRONTIERS IN IMMUNOLOGY
(2022)
