Article
Plant Sciences
Olga Kershanskaya, Gulvira L. Yessenbaeva, Darya S. Nelidova, Aizhan N. Karabekova, Zarina N. Sadullaeva
Summary: The CRISPR/Cas9 technology has the potential to improve crop breeding efficiency and antiviral defense, and its application in barley breeding has shown promising results.
PHYSIOLOGIA PLANTARUM
(2022)
Review
Plant Sciences
M. Ahmad
Summary: Genome editing techniques are used to modify plant breeding and increase food production sustainably by 2050. The development of plants and food production has been greatly impacted by global warming and climate change, making it crucial to minimize these effects for sustainable agriculture. Recently, plant breeders have shown interest in genome editing approaches, especially using CRISPR/Cas9, to develop plant varieties with desired traits and ensure food security in the future.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Rim Lassoued, Peter W. B. Phillips, Diego Maximiliano Macall, Hayley Hesseln, Stuart J. Smyth
Summary: Global food security is influenced by various factors, with genome editing playing an important role in agricultural research. The potential and market growth of new breeding techniques like genome editing face challenges, mainly due to international regulatory, ethical, and societal constraints.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Pharmacology & Pharmacy
Abhijit Dey
Summary: Medicinal plants have been historically significant for human use, and the CRISPR/Cas9 genome editing tool has been successfully applied to engineer plant secondary metabolic pathways, enhancing the production of genetically-engineered medicinal crops with advanced traits.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Plant Sciences
Satoru Sukegawa, Hiroaki Saika, Seiichi Toki
Summary: The development of CRISPR/Cas9 system has revolutionized biology by enabling targeted mutagenesis, gene targeting, and chromosome engineering. Genome-editing technologies have advanced rapidly, allowing precise gene modifications and rearrangement of genomes and chromosomes in plants.
Review
Biochemistry & Molecular Biology
Emre Aksoy, Kubilay Yildirim, Musa Kavas, Ceyhun Kayihan, Bayram Ali Yerlikaya, Irmak Calik, Ilkay Sevgen, Ufuk Demirel
Summary: CRISPR/Cas technology is a versatile genome editing tool that has been used to improve agriculturally important plant traits. It is more effective than conventional breeding methods or genetic engineering for developing climate resilient plant varieties. Knowledge in tissue culture-based plant transformation, effective sgRNA design, off-target prediction, and use of vectors, promoters, Cas proteins, and terminators is necessary. Various bioinformatics tools are available for sgRNA design and off-target screening.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Plant Sciences
Tien Van Vu, Swati Das, Goetz Hensel, Jae-Yean Kim
Summary: Genome editing technology, particularly CRISPR-Cas, offers a promising solution for plant breeding to ensure food production for the growing world population. This technology is gaining recognition worldwide and has the potential to address the challenges posed by climate change and the depletion of natural genetic resources. The application of CRISPR-Cas technology in plant breeding is seen as a powerful tool and can offer new perspectives for the future of food production.
Review
Biochemistry & Molecular Biology
Hyun-Min Kim, Yebin Hong, Jiani Chen
Summary: CRISPR-Cas enables highly efficient genome editing, allowing the introduction of desired modifications such as mutations and deletions. C. elegans has emerged as a significant model organism for CRISPR-Cas, with numerous strategies established. However, the abundance of methods makes it challenging to choose the correct approach, highlighting the importance of selecting appropriate screening methods for genome modification planning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Simran Bhatia, Sudesh Kumar Pooja, Sudesh Kumar Yadav
Summary: The CRISPR-Cas system is an adaptive immune response mechanism in prokaryotes that integrates short sequences of the target genome (spacers) into the CRISPR locus. CRISPR-Cas, classified based on Cas proteins, uses programmable RNAs to target DNA sequences and has become a powerful technique in genome editing. This article discusses the evolution, classification, design, and molecular mechanism of CRISPR-Cas systems, as well as their applications in agriculture and anticancer therapy. It also briefly explores the role of CRISPR and its Cas systems in the diagnosis and prevention of COVID-19, and discusses the challenges and potential solutions in existing CRISPR-Cas technologies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Syed Inzimam Ul Haq, Dianfeng Zheng, Naijie Feng, Xingyu Jiang, Feng Qiao, Jin-Sheng He, Quan-Sheng Qiu
Summary: This review summarizes the advances of CRISPR/Cas9 technology in plant genome editing and discusses its applications in forage breeding, as well as the potential and challenges.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Muhammad Naeem, Mubasher Zahir Hoque, Muhammad Ovais, Chanbasha Basheer, Irshad Ahmad
Summary: The innovative CRISPR-Cas technology has enabled genetic engineers to manipulate genomes effectively, but barriers in clinical applications remain, including efficient delivery and off-target effects. Various viral and non-viral vectors are designed to address these issues, but challenges such as immune response and lack of specificity persist.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Xu Tang, Yong Zhang
Summary: The use of CRISPR-Cas genome editing technology in plant research is rapidly expanding, particularly in the field of editing plant promoters. However, there are significant limitations in using CRISPR-Cas9 to edit noncoding sequences like promoters, which have unique structures and regulatory mechanisms. Researchers urgently need efficient editing tools and strategies to overcome these obstacles and achieve precise target gene expression regulation. This article provides insights and references for implementing promoter editing-based research in plants.
Review
Microbiology
Ruben D. Arroyo-Olarte, Ricardo Bravo Rodriguez, Edgar Morales-Rios
Summary: CRISPR-Cas technology has revolutionized genome editing in eukaryotic organisms with its simplicity and programmability, but faces challenges in bacterial genome editing. Alternative approaches are being explored to overcome these issues and expand the application of genome editing in bacteria.
Review
Plant Sciences
Michelle Roenspies, Patrick Schindele, Holger Puchta
Summary: The powerful CRISPR/Cas system has revolutionized plant breeding by enabling precise genome manipulation, moving beyond simply knocking in or out single genes to inducing targeted chromosomal rearrangements. This technique has the potential to transform plant breeding by altering genetic linkages between traits and overcoming natural obstacles to breeding processes like inversions. Recent breakthroughs in chromosome engineering in plants suggest potential applications in shaping plant chromosomes based on breeding needs.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Environmental Sciences
Suvra Roy, Vikash Kumar, Bijay Kumar Behera, Janmejay Parhi, Sipra Mohapatra, Tapas Chakraborty, Basanta Kumar Das
Summary: Fisheries and aquaculture, as the fastest-growing food-producing sector, can benefit from genome editing technology like CRISPR/Cas9 for sustainable genetic improvement. However, there are technical, regulatory, and public challenges to be overcome.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Organic
Willem A. Velema, Jan Pieter van der Berg, Wiktor Szymanski, Arnold J. M. Driessen, Ben L. Feringa
ORGANIC & BIOMOLECULAR CHEMISTRY
(2015)
Article
Chemistry, Multidisciplinary
J. P. Van der Berg, W. A. Velema, W. Szymanski, A. J. M. Driessen, B. L. Feringa
Article
Biochemistry & Molecular Biology
Willem A. Velema, Jan Pieter van der Berg, Wiktor Szymanski, Arnold J. M. Driessen, Ben L. Feringa
ACS CHEMICAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Claire E. Price, Armagan Kocer, Stefan Kol, Jan Pieter van der Berg, Arnold J. M. Driessen
Article
Biochemistry & Molecular Biology
Stefan Kol, Wiktor Majczak, Rene Heerlien, Jan Pieter van der Berg, Nico Nouwen, Arnold J. M. Driessen
JOURNAL OF MOLECULAR BIOLOGY
(2009)
Article
Chemistry, Multidisciplinary
Willem A. Velema, Jan Pieter van der Berg, Mickel J. Hansen, Wiktor Szymanski, Arnold J. M. Driessen, Ben L. Feringa
Article
Multidisciplinary Sciences
Jan Pieter van der Berg, Pramod Kumar Madoori, Amalina Ghaisani Komarudin, Andy-Mark Thunnissen, Arnold J. M. Driessen
Article
Reproductive Biology
Jan Pieter van der Berg, Gijs A. Kleter, Esther J. Kok
Article
Environmental Sciences
Jan Pieter van der Berg, Gijs A. Kleter, Evy Battaglia, Lianne M. S. Bouwman, Esther J. Kok
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Review
Food Science & Technology
J. L. Banach, J. P. van der Berg, G. Kleter, H. van Bokhorst-van de Veen, S. Bastiaan-Net, L. Pouvreau, E. D. van Asselt
Summary: This review provides an analysis of the food safety hazards, industry trends, and processing methods associated with alternative proteins for meat and dairy products in the European Union market. Understanding the effects of processing and safety of alternative proteins is crucial for ensuring food safety and assessing risks to consumers, though current data is limited.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Review
Biochemical Research Methods
Gijs A. Kleter, Hilko van der Voet, Jasper Engel, Jan-Pieter van der Berg
Summary: The initial compositional analysis of plants is crucial in the internationally harmonized approach to assess the safety of genetically modified plants. The current EFSA guidance suggests two types of comparison methods: difference tests with a conventional comparator or control, and equivalence tests with a collection of commercial reference varieties. Previous experience shows that statistically significant differences between the test and control can often be ignored if they fall within the equivalence limits of reference varieties with a presumed safe history. Thus, the inclusion of a conventional counterpart and the performance of difference testing can be omitted in favor of including safety testing regimes into other variety trials.
TRANSGENIC RESEARCH
(2023)
Review
Agriculture, Multidisciplinary
Mark H. J. Sturme, Jan Pieter van der Berg, Lianne M. S. Bouwman, Adinda De Schrijver, Ruud A. de Maagd, Gijs A. Kleter, Evy Battaglia-de Wilde
Summary: CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants, but unintended off-target edits can increase risks for gene-edited food crops. Most observed off-target changes are small insertions, deletions, or nucleotide substitutions, with large deletions being rare. Off-target sites have few mismatches with the target sequence and are mainly located in protein-coding regions, often in target gene homologues. Off-target edits are primarily detected through biased analysis of predicted off-target sites. CRISPR-Cas-edited plants show lower off-target mutation frequencies compared to conventionally bred plants. This review can assist in discussing the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)
Review
Agronomy
Jan Pieter van der Berg, Gijs A. Kleter, Evy Battaglia, Martien A. M. Groenen, Esther J. Kok
FRONTIERS OF AGRICULTURAL SCIENCE AND ENGINEERING
(2020)