Pharma 4.0 Continuous mRNA Drug Products Manufacturing

Correction: Kis, Z. et al. Resources, Production Scales and Time Required for Producing RNA Vaccines for the Global Pandemic Demand. Vaccines 2021, 9, 3

(2021) Zoltán Kis et al.   Vaccines

Digital Twin of mRNA-Based SARS-COVID-19 Vaccine Manufacturing towards Autonomous Operation for Improvements in Speed, Scale, Robustness, Flexibility and Real-Time Release Testing

(2021) Axel Schmidt et al.   Processes

Development of a Nanocrystal Formulation of a Low Melting Point API Following a Quality by Design Approach

(2021) Andreas Ouranidis et al.   Processes

Biopharmaceutics 4.0, Advanced Pre-Clinical Development of mRNA-Encoded Monoclonal Antibodies to Immunosuppressed Murine Models

(2021) Andreas Ouranidis et al.   Vaccines

Design of novel multiepitope constructs-based peptide vaccine against the structural S, N and M proteins of human COVID-19 using immunoinformatics analysis

(2020) Niloofar Khairkhah et al.   PLoS One

Addressing the Cold Reality of mRNA Vaccine Stability

(2020) Daan J.A. Crommelin et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Manufacturing Considerations for the Development of Lipid Nanoparticles Using Microfluidics

(2020) Carla B. Roces et al.   Pharmaceutics

Why Is Batch Processing Still Dominating the Biologics Landscape? Towards an Integrated Continuous Bioprocessing Alternative

(2020) Ashish Kumar et al.   Processes

Resources, Production Scales and Time Required for Producing RNA Vaccines for the Global Pandemic Demand

(2020) Zoltán Kis et al.   Vaccines

Comparison between Batch and Continuous Monoclonal Antibody Production and Economic Analysis

(2019) Ou Yang et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA

(2019) Markus Baiersdörfer et al.   Molecular Therapy-Nucleic Acids

mRNA vaccines — a new era in vaccinology

(2018) Norbert Pardi et al.   NATURE REVIEWS DRUG DISCOVERY

Lifetime and Aging of Chromatography Resins during Biopharmaceutical Manufacture

(2018) Mauryn C. Nweke et al.   TRENDS IN BIOTECHNOLOGY

3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses

(2018) Yasaman Gholamalipour et al.   NUCLEIC ACIDS RESEARCH

Techno-economic evaluation of a complete bioprocess for 2,3-butanediol production from renewable resources

(2016) Apostolis A. Koutinas et al.   BIORESOURCE TECHNOLOGY

Cost evaluation of antibody production processes in different operation modes

(2016) Stephan Klutz et al.   CHEMICAL ENGINEERING SCIENCE

Elastic deformation and area per lipid of membranes: Atomistic view from solid-state deuterium NMR spectroscopy

(2015) Jacob J. Kinnun et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Techno-economic analysis of a food waste valorization process via microalgae cultivation and co-production of plasticizer, lactic acid and animal feed from algal biomass and food waste

(2015) Tsz Him Kwan et al.   BIORESOURCE TECHNOLOGY

mRNA: Fulfilling the Promise of Gene Therapy

(2015) Drew Weissman et al.   MOLECULAR THERAPY

Design and Operation of Continuous Countercurrent Chromatography in Biotechnological Production

(2014) Steffen Zobel et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

The Amazing Ability of Continuous Chromatography To Adapt to a Moving Environment

(2014) Roger-Marc Nicoud   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

mRNA-based therapeutics — developing a new class of drugs

(2014) Ugur Sahin et al.   NATURE REVIEWS DRUG DISCOVERY

Improving affinity chromatography resin efficiency using semi-continuous chromatography

(2012) Ekta Mahajan et al.   JOURNAL OF CHROMATOGRAPHY A

Techno-economic evaluation of an inclusion body solubilization and recombinant protein refolding process

(2011) Esteban J. Freydell et al.   BIOTECHNOLOGY PROGRESS

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

(2011) Katalin Karikó et al.   NUCLEIC ACIDS RESEARCH

Large scale purification of linear plasmid DNA for efficient high throughput cloning

(2010) Marjolaine Noirclerc-Savoye et al.   Biotechnology Journal

Size exclusion chromatography of plasmid DNA isoforms

(2009) David R. Latulippe et al.   JOURNAL OF CHROMATOGRAPHY A

A mechanistic study of Protein A chromatography resin lifetime

(2009) Canping Jiang et al.   JOURNAL OF CHROMATOGRAPHY A

PACKMOL: A package for building initial configurations for molecular dynamics simulations

(2009) L. Martínez et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Optimal Economic Design and Operation of Single- and Multi-column Chromatographic Processes

(2008) S. Chan et al.   BIOTECHNOLOGY PROGRESS

Simulated moving bed chromatography for the separation of enantiomers

(2008) Arvind Rajendran et al.   JOURNAL OF CHROMATOGRAPHY A

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY