Review
Engineering, Biomedical
Ian T. Whelan, E. Moeendarbary, David A. Hoey, Daniel J. Kelly
Summary: The emerging microphysiological systems (MPS) technology allows for the engineering of more physiologically relevant vasculature in vitro, potentially impacting the development of bone research. Engineering vasculature within the specific design constraints of the bone niche is challenging. Researchers are working towards providing technical guidance for the biofabrication of vascularised bone tissue within MPS devices.
Review
Cell Biology
Lakyn N. Mayo, Matthew L. Kutys
Summary: Tumor metastasis through the circulation requires crossing the vascular barrier twice. Recent advances in microphysiological systems have provided a new approach to study the mechanisms underlying tumor-endothelial interactions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Neurosciences
Edward R. Neves, Brendan A. C. Harley, Sara Pedron
Summary: Brain tumors lack effective treatments, and understanding tumor progression and resistance mechanisms is unclear. Technological advances in in vitro modeling are being used to increase the efficiency of preclinical systems. Microphysiological brain tumor models may offer insights into disease mechanisms and predict patient response to anticancer treatments.
BRAIN RESEARCH BULLETIN
(2021)
Article
Engineering, Biomedical
Ian T. Whelan, Ross Burdis, Somayeh Shahreza, Emad Moeendarbary, David A. Hoey, Daniel J. Kelly
Summary: Endochondral ossification (EO) is a critical biological process for bone development and healing. However, understanding of this process is limited, preventing effective treatment of dysregulated EO. The lack of predictive in vitro models is a key factor, and microphysiological systems offer an advanced platform for research. In this study, a microphysiological model of vascular invasion into developing/regenerating bone was developed, mimicking the process of EO. This model successfully replicated key events in EO and could be used for further research and drug testing.
Review
Biochemistry & Molecular Biology
Georgia Gomatou, Nikolaos Syrigos, Ioannis A. Vathiotis, Elias A. Kotteas
Summary: Tumor dormancy is a critical stage of cancer development where tumor cells are present but cancer does not progress. It involves both cellular and tumor mass dormancy, which are fundamental in preventing recurrence and maximizing therapeutic benefit.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Physical
Shuyu Zhang, Guoshi Xu, Juan Wu, Xiao Liu, Yong Fan, Jun Chen, Gordon Wallace, Qi Gu
Summary: In recent years, significant advancements have been made in microphysiological constructs and systems (MPCs and MPSs), providing alternatives to animal models for drug discovery and personalized medicine. However, current approaches mainly focus on the in vitro recapitulation of the human anatomical structure and physiological-biochemical indices at a single or a few simple levels.
Review
Chemistry, Multidisciplinary
Hanie Kavand, Rohollah Nasiri, Anna Herland
Summary: Advanced in vitro cell culture systems and microphysiological systems (MPSs) are breakthrough technologies in biomedicine that replicate features of human tissues. The use of advanced functional materials and devices could enable better reproduction of in vivo-like functionality and real-time monitoring of tissue function, leading to more accurate mimicry of human physiology.
ADVANCED MATERIALS
(2022)
Article
Gastroenterology & Hepatology
Athanasia Apostolou, Rohit A. Panchakshari, Antara Banerjee, Dimitris Manatakis, Maria D. Paraskevopoulou, Raymond Luc, Galeb Abu-Ali, Alexandra Dimitriou, Carolina Lucchesi, Gauri Kulkarni, Tengku Ibrahim Maulana, Magdalena Kasendra, Jordan S. Kerns, Bertram Bleck, Lorna Ewart, Elias S. Manolakos, Geraldine A. Hamilton, Cosmas Giallourakis, Katia Karalis
Summary: This study developed a microphysiological Colon Intestine-Chip platform that can mimic properties of human intestinal epithelium. It highlights the importance of endothelium and in vivo tissue-relevant dynamic microenvironment in establishing a tight monolayer of differentiated, polarized, organoid-derived intestinal epithelial cells, providing insights into the mechanism of interleukin 22 in the human epithelial barrier.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Oncology
Amanda M. Clark, Nancy L. Allbritton, Alan Wells
Summary: The liver is the most commonly involved organ in metastases from various solid tumors, and studying tumor cell behavior in the liver microenvironment has provided unexpected insights into the biology of metastasis. Microphysiological systems linking different organs can help examine the influence of one organ on the function of other tissues, including how the liver environment affects tumor behavior.
SEMINARS IN CANCER BIOLOGY
(2021)
Review
Engineering, Biomedical
Jun-ya Shoji, Richard P. Davis, Christine L. Mummery, Stefan Krauss
Summary: Organoids and cells in organ-on-chip platforms replicate higher-level anatomical, physiological, or pathological states of tissues and organs. A meta-analysis based on a quality-controlled text-mining algorithm is performed to map advances in this emerging field. The analysis covers academic publications in the literature and preprint databases published after 2010, and provides a robust framework for researchers to interrogate with their own questions.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Leandra S. Baptista, Constance Porrini, Gabriela S. Kronemberger, Daniel J. Kelly, Cecile M. Perrault
Summary: Medicine today faces the challenge of increasing untreatable diseases and a decrease in successful drug development. In vitro preclinical tests, particularly using 3D cell culture and human stem cell biology, can help predict the potential of new drugs and avoid expensive clinical trial phases. Integrating organoid culture with microsystems, such as microphysiological systems or organ-on-a-chip, can improve drug development by emulating physiological conditions and reducing costs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Mridu Malik, Yang Yang, Parinaz Fathi, Gretchen J. Mahler, Mandy B. Esch
Summary: Traditional animal models for drug identification are facing criticism for their reliability in predicting drug toxicity and efficacy in humans. Multi-organ microphysiological systems (MPS) are considered as alternative models which can simulate diseases and evaluate the efficacy and safety of drug candidates, but accuracy in organ scaling and flow rates is crucial.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mitchell E. Fane, Yash Chhabra, Gretchen M. Alicea, Devon A. Maranto, Stephen M. Douglass, Marie R. Webster, Vito W. Rebecca, Gloria E. Marino, Filipe Almeida, Brett L. Ecker, Daniel J. Zabransky, Laura Huser, Thomas Beer, Hsin-Yao Tang, Andrew Kossenkov, Meenhard Herlyn, David W. Speicher, Wei Xu, Xiaowei Xu, Elizabeth M. Jaffee, Julio A. Aguirre-Ghiso, Ashani T. Weeraratna
Summary: The aged lung microenvironment provides a permissive niche for the growth and dissemination of melanoma cells, while age-related changes in the skin suppress melanoma growth but drive dissemination. Reprogramming of lung fibroblasts and activation of WNT5A promote the metastasis and dissemination of melanoma cells by inhibiting WNT5A in melanoma cells.
Article
Biophysics
Youngwon Kim, Jaehun Lee, Sunghan Lee, Hyo-Il Jung, Bongseop Kwak
Summary: Microphysiological systems (MPS) are powerful tools for in vitro disease validation, enhancing understanding of tumors and their microenvironments. The newly developed MPS described here consists of a binary tumor-microenvironment-on-a-chip system that allows for precise drug validation and connection of compartments.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Engineering, Biomedical
Giridharan Muniraj, Rachel Hui Shuen Tan, Yichen Dai, Ruige Wu, Massimo Alberti, Gopu Sriram
Summary: Researchers have developed an in vitro microphysiological platform to mimic the complex microenvironment of gingiva. The platform allows for the biofabrication of stable gingival equivalents under long-term and air-liquid interface culture with flow conditions. The platform also models a diseased state with disrupted barrier function and assesses the transmucosal permeation of oral-care formulations. The platform provides a miniaturized and integrated platform for host-material and host-microbiome applications in gingival and oral mucosa research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Marianna Sofman, Alexander Brown, Linda G. Griffith, Paula T. Hammond
Summary: Three-dimensional micro-physiological in vitro models, such as embedding degradable hydrogel microbeads coated with induced pluripotent stem cell-derived endothelial cells, have the potential to drive angiogenesis and support vascularization of cell-dense tissues with high metabolic demand.
Article
Obstetrics & Gynecology
Megan Loring, Zachary Kabelac, Usman Munir, Shichao Yue, Hannah Y. Ephraim, Hariharan Rahul, Keith B. Isaacson, Linda G. Griffith, Dina Katabi
Summary: This study aimed to assess the feasibility of a noncontact radio sensor as an objective measurement tool for postoperative recovery from endometriosis surgery, and found a significant correlation between sleep variables and pain scores.
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2021)
Article
Oncology
Amanda M. Clark, Nancy L. Allbritton, Alan Wells
Summary: The liver is the most commonly involved organ in metastases from various solid tumors, and studying tumor cell behavior in the liver microenvironment has provided unexpected insights into the biology of metastasis. Microphysiological systems linking different organs can help examine the influence of one organ on the function of other tissues, including how the liver environment affects tumor behavior.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Immunology
Amelia P. Bailey, Abby S. Hill, Michael T. Beste, Christi D. Cook, Vishnudas Sarda, Marc R. Laufer, Keith B. Isaacson, Linda G. Griffith, Stacey A. Missmer
Summary: The study compared peritoneal cytokines in adolescents and adults with and without endometriosis. The results showed that adolescents with endometriosis have a more pro-invasion cytokine profile compared to adults with or without the condition. This suggests unique peritoneal cytokine profiles and molecular behavior in adolescents with endometriosis.
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Wen Li Kelly Chen, Emily Suter, Hikaru Miyazaki, Jason Velazquez, Douglas A. Lauffenburger, Linda G. Griffith, Rebecca L. Carrier
Summary: This study utilized a human intestinal model and analyzed cellular communication methods to demonstrate that NSAID use may disrupt intestinal barrier function and induce inflammatory responses; while comprehensive inflammation suppression alone cannot achieve mucosal healing, recovery of intestinal functions can gradually occur after NSAID removal.
ACS INFECTIOUS DISEASES
(2021)
Article
Medicine, Research & Experimental
Amanda M. Clark, Chandi Magawa, Adriana Pliego-Zamora, Pauline Low, Max Reynolds, Stephen J. Ralph
Summary: MAC exhibits anticancer activity against prostate and breast cancer cells by inducing apoptosis and cell cycle arrest, as well as inducing breast cancer cell death through the mitochondrial apoptotic pathway. It also suppresses tumor progression and promotes infiltration of neutrophils with anticancer cytotoxic activity in breast tumors.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Oncology
Juan Luis Gomez Marti, Colin H. Beckwitt, Amanda M. Clark, Alan Wells
Summary: The study examined the effects of atorvastatin on chemotherapy for mTNBC through animal models and clinical correlations. Atorvastatin was found to have additive effects with doxorubicin targeting liver metastases, and the combination of atorvastatin and chemotherapy led to increased cell death in dormant cancer cells.
BRITISH JOURNAL OF CANCER
(2021)
Article
Chemistry, Physical
Christopher R. Below, Joanna Kelly, Alexander Brown, Jonathan D. Humphries, Colin Hutton, Jingshu Xu, Brian Y. Lee, Celia Cintas, Xiaohong Zhang, Victor Hernandez-Gordillo, Linda Stockdale, Matthew A. Goldsworthy, Joe Geraghty, Lucy Foster, Derek A. O'Reilly, Barbara Schedding, Janet Askari, Jessica Burns, Nigel Hodson, Duncan L. Smith, Catherine Lally, Garry Ashton, David Knight, Aleksandr Mironov, Antonia Banyard, Johannes A. Eble, Jennifer P. Morton, Martin J. Humphries, Linda G. Griffith, Claus Jorgensen
Summary: A synthetic hydrogel has been developed to mimic the physicochemical properties of pancreatic tissue and is shown to support the culture of pancreatic cancer organoids, revealing the role of laminin-integrin interactions in their growth.
Article
Cell & Tissue Engineering
Haiting Ma, Esmee de Zwaan, Yang Eric Guo, Paloma Cejas, Prathapan Thiru, Martijn van de Bunt, Jacob F. Jeppesen, Sudeepa Syamala, Alessandra Dall'Agnese, Brian J. Abraham, Dongdong Fu, Carrie Garrett-Engele, Tong Ihn Lee, Henry W. Long, Linda G. Griffith, Richard A. Young, Rudolf Jaenisch
Summary: By comparing gene regulatory elements, we found that the thyroid receptor THRB plays an important role in the maturation of hPSC-derived hepatocytes, and the thyroid hormone T3 can enhance the binding of THRB and gene expression. The resulting hPSC-hepatocytes showed a closer epigenetic status and gene expression to primary hepatocytes, and could be successfully engrafted into the mouse liver.
Meeting Abstract
Endocrinology & Metabolism
Erin Tevonian, Jacob F. Jeppesen, Douglas Lauffenburger, Linda G. Griffith
Article
Obstetrics & Gynecology
Hilde Jorgensen, Peter Fedorcsak, Keith Isaacson, Erin Tevonian, Amy Xiao, Michael Beste, Erik Qvigstad, Douglas Lauffenburger, Linda Griffith
Summary: By analyzing the concentrations of cytokines, chemokines, and growth factors in endometrial biopsy specimens, it is possible to distinguish between patients with endometriosis and those without, and infer dysregulated immune networks.
FERTILITY AND STERILITY
(2022)
Correction
Cell & Tissue Engineering
Haiting Ma, Esmee de Zwaan, Yang Eric Guo, Paloma Cejas, Prathapan Thiru, Martijn van de Bunt, Jacob F. Jeppesen, Sudeepa Syamala, Alessandra Dall'Agnese, Brian J. Abraham, Dongdong Fu, Carrie Garrett-Engele, Tong Ihn Lee, Henry W. Long, Linda G. Griffith, Richard A. Young, Rudolf Jaenisch
Article
Oncology
Christopher M. Rota, Alexander T. Brown, Emily Addleson, Clara Ives, Ella Trumper, Kristine Pelton, Wei Pin Teh, Matthew J. Schniederjan, Robert Craig Castellino, Sara Buhrlage, Douglas A. Lauffenburger, Keith L. Ligon, Linda G. Griffith, Rosalind A. Segal
Summary: The study developed a synthetic extracellular matrix (sECM) culture system for growing and studying pediatric glioma cells. The system allowed for the proliferation of various pediatric brain tumors and enabled testing of investigational therapeutics. The sECM material also allowed for the evaluation of the invasion of high-grade pediatric glioma cells and therapeutic interventions.
NEURO-ONCOLOGY ADVANCES
(2022)
Meeting Abstract
Respiratory System
Sofia Winslow, Llywelyn Griffith, Henric Olsson, Jernej Ule, Zala Jevnikar Rojnik
EUROPEAN RESPIRATORY JOURNAL
(2021)