Review
Biochemistry & Molecular Biology
Vittoria Rago, Anna Perri, Silvia Di Agostino
Summary: One of the major goals in cancer research is to develop new anticancer therapies. Preclinical in vitro models, such as patient-derived xenograft (PDX) models and cultured organoids derived from patients (PDO), have become indispensable tools for understanding cancer progression, drug resistance, and metastasis. This article reviews studies from the past 10 years on the use of PDX models and PDOs in studying prostate cancer, which is characterized by genomic instability and resistance to conventional therapies. The limitations and new challenges in using these models for drug testing and developing molecular therapies are also discussed.
Article
Oncology
Jerome Cartry, Sabrina Bedja, Alice Boileve, Jacques R. R. Mathieu, Emilie Gontran, Maxime Annereau, Bastien Job, Ali Mouawia, Pierre Mathias, Thierry De Baere, Antoine Italiano, Benjamin Besse, Isabelle Sourrouille, Maximiliano Gelli, Mohamed-Amine Bani, Peggy Dartigues, Antoine Hollebecque, Cristina Smolenschi, Michel Ducreux, David Malka, Fanny Jaulin
Summary: The study shows that PDO-based drug testing can be conducted in routine clinical practice. The chemogram could serve as a decision-making tool for clinicians to personalize patient treatment. Therefore, PDO-based functional precision oncology should be further tested in interventional trials to assess its clinical utility in patients without actionable genomic alterations or resistance to standard of care treatments.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Review
Health Care Sciences & Services
Mark N. Pernik, Cylaina E. Bird, Jeffrey Traylor, Diana D. Shi, Timothy E. Richardson, Samuel K. McBrayer, Kalil G. Abdullah
Summary: The emergence of patient-derived cancer organoid (PDO) models, which closely recapitulate parental tumor tissue, provides a promising approach in cancer research. PDOs can efficiently retain tumor-specific properties and enhance precision oncologic care for patients compared to traditional cell lines and xenograft models. This review highlights the advantages of PDOs in cancer modeling and potential clinical applications.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Sahithi Pamarthy, Hatem E. Sabaawy
Summary: Advancements in utilizing patient derived organoids (PDOs) for modeling prostate cancer have shown promise in predicting therapeutic responses and screening effective targeted therapies. Technological innovations in generating PDOs and incorporating various extracellular matrices have improved the accuracy and reliability of these models. PDOs, especially those derived from single cells, accurately represent the molecular features of the original tumor and serve as a reliable pre-clinical model for understanding individual tumors and designing tailored targeted therapies.
Review
Oncology
Mikaela Gronholm, Michaela Feodoroff, Gabriella Antignani, Beatriz Martins, Firas Hamdan, Vincenzo Cerullo
Summary: Cancer immunotherapy has transformed tumor treatment, but there is a lack of efficient testing platforms for pretreatment evaluation of therapies and understanding the tumor microenvironment. Patient-derived tumor organoid cultures and complex immune-organoid cultures offer potential for preclinical testing platforms in precision cancer immunotherapy.
Review
Chemistry, Physical
Lanxiang Huang, Yaqi Xu, Na Wang, Kezhen Yi, Xiaodan Xi, Huaqi Si, Qian Zhang, Ming Xiang, Yuan Rong, Yufeng Yuan, Fubing Wang
Summary: This article discusses the advantages and applications of patient-derived tumor organoids and highlights the biological functions of circulating tumor cells. It also provides an overview of the latest advancements in research on circulating tumor cell-derived organoids and discusses the potential applications and challenges of this technology.
Article
Biochemistry & Molecular Biology
Emilie Darrigues, Edward H. Zhao, Annick De Loose, Madison P. Lee, Michael J. Borrelli, Robert L. Eoff, Deni S. Galileo, Narsimha R. Penthala, Peter A. Crooks, Analiz Rodriguez
Summary: This study screened potential GBM therapeutic drugs using patient-derived 3D models, finding that tubulin inhibitor had the best efficacy in U-251 MG cells but showed highly variable results in patient organoids. The efficacy of compounds was found to be closely related to patient tumor heterogeneity, suggesting that such models can be used to evaluate individualized cancer treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Federica Papaccio, Manuel Cabeza-Segura, Blanca Garcia-Mico, Noelia Tarazona, Desamparados Roda, Josefa Castillo, Andres Cervantes
Summary: Precision medicine approaches for solid tumors, mainly based on genomics, have shown limited success. Factors such as gene expression, genomic alterations, and the tumor microenvironment can all influence the response, making a genomics-only approach insufficient. However, the implementation of patient-derived organoids shows promise in predicting patient response to drug treatment.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Ji Wang, Chao Chen, Lu Wang, Mingjun Xie, Xinyang Ge, Sufan Wu, Yong He, Xiaozhou Mou, Chenyang Ye, Yi Sun
Summary: Cancer immunotherapy has revolutionized cancer treatment, but durable responses are not observed in all patients, highlighting the need for reliable testing platforms. Patient-derived tumor organoids serve as preclinical models for cancer research and precision medicine, yet translating them into clinical decision making presents challenges. Furthermore, tumor organoids can be used with novel multi-omics techniques such as genetic editing, proteomics, and liquid biopsy.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Evangelia Sereti, Irida Papapostolou, Konstantinos Dimas
Summary: Despite its aggression and resistance to treatment, pancreatic ductal adenocarcinoma (PDAC) can potentially benefit from the development of organoid culture systems. These PDAC organoids have the potential to bridge the gap between in vitro and in vivo models, making them powerful preclinical tools for studying the biology, progression, and treatment response of pancreatic cancer. This review highlights the importance of PDAC organoids in advancing precision medicine for this deadly malignancy.
Article
Oncology
Federica Papaccio, Blanca Garcia-Mico, Francisco Gimeno-Valiente, Manuel Cabeza-Segura, Valentina Gambardella, Maria Fernanda Gutierrez-Bravo, Clara Alfaro-Cervello, Carolina Martinez-Ciarpaglini, Pilar Rentero-Garrido, Sheila Zuniga-Trejos, Juan Antonio Carbonell-Asins, Tania Fleitas, Susana Rosello, Marisol Huerta, Manuel M. Sanchez M. del Pino, Luis Sabater, Desamparados Roda, Noelia Tarazona, Andres Cervantes, Josefa Castillo
Summary: This study generated patient-derived organoids (PDOs) from advanced colorectal cancer (CRC) patients and integrated drug response with multi-omics characterization. By using SWATH-MS and RNA-seq analysis, they identified baseline proteins and gene expression profiles that could predict treatment response and aid in the development of personalized cancer therapeutics.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Margalida Torrens-Mas, Catalina Perello-Reus, Cayetano Navas-Enamorado, Lesly Ibarguen-Gonzalez, Andres Sanchez-Polo, Juan Jose Segura-Sampedro, Luis Masmiquel, Carles Barcelo, Marta Gonzalez-Freire
Summary: The field of biology of aging focuses on discovering new pathways to delay aging and extend lifespan. Research in this field has primarily been done in animal models, yeast, Caenorhabditis elegans, and cell cultures, raising questions about the transferability of findings to humans. Organoid culture technology, used in cancer research, has great potential as a preclinical model for studying aging and discovering new biomarkers, predicting adverse outcomes, and designing personalized approaches for aging-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Health Care Sciences & Services
Masahiro Shiihara, Toru Furukawa
Summary: Cell models play a crucial role in the research and development of cancer therapies. Recent advances in patient-derived cancer organoids have provided a valuable platform for studying cancer biology and personalized medicine, particularly in the field of pancreatobiliary cancer.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Christopher Fabian Maier, Lei Zhu, Lahiri Kanth Nanduri, Daniel Kuehn, Susan Kochall, May-Linn Thepkaysone, Doreen William, Konrad Grutzmann, Barbara Klink, Johannes Betge, Jurgen Weitz, Nuh N. Rahbari, Christoph Reissfelder, Sebastian Schoelch
Summary: This study introduces a novel protocol for cholangiocarcinoma patient-derived organoid culture and transition to 2D cell lines, demonstrating their potential in evaluating new treatments. Patient-derived organoids were shown to faithfully recapitulate the original features of primary tumors on multiple levels, providing new resources for personalized treatment research in cholangiocarcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Kristina Aaltonen, Katarzyna Radke, Aleksandra Adamska, Alexandra Seger, Adriana Manas, Daniel Bexell
Summary: Neuroblastoma is a childhood cancer derived from the sympathetic nervous system. High-risk neuroblastoma patients have a poor overall survival and account for similar to 15% of childhood cancer deaths. There is thus a need for clinically relevant and authentic models of neuroblastoma that closely resemble the human disease to further interrogate underlying mechanisms and to develop novel therapeutic strategies.
FRONTIERS IN ONCOLOGY
(2023)
Article
Gastroenterology & Hepatology
Hyuk Lee, Byung-Hoon Min, Jeong Hoon Lee, Cheol Min Shin, Younjoo Kim, Hyunsoo Chung, Sang Hyub Lee
AMERICAN JOURNAL OF GASTROENTEROLOGY
(2015)
Meeting Abstract
Gastroenterology & Hepatology
Hyuk Lee, Byung-Hoon Min, Jeong Hoon Lee, Cheol Min Shin, Younjoo Kim, Sang Hyub Lee
GASTROINTESTINAL ENDOSCOPY
(2015)
Article
Oncology
Misun Park, Junhye Kwon, Hye-Jin Shin, Sun Mi Moon, Sang Bum Kim, Ui Sup Shin, Young-Hoon Han, Younjoo Kim
INTERNATIONAL JOURNAL OF ONCOLOGY
(2020)
Article
Cell Biology
Misun Park, Junhye Kwon, Heejeong Youk, Ui Sup Shin, Young-Hoon Han, Younjoo Kim
Summary: The study identifies valproic acid (VPA) as a potent radioprotector for LGR5+ stem cells through NOTCH signaling, while also acting as a radiosensitizer in colorectal cancer organoids by inhibiting histone deacetylase (HDAC).
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Jong Il Lee, Sang Sik Cho, Ui Sup Shin, Byong Ho Jeon, Sun Mi Moon, Younjoo Kim, Ki Young Yang, Byung Il Kim
Summary: The study investigated postoperative colonoscopy results in patients with left-sided stenosing CRC without synchronous FDG-avid lesions. The cumulative risks of advanced adenoma or carcinoma were higher in all patients at 1 and 5 years after surgery, but the additional surgical risk did not differ significantly between groups.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Misun Park, Junhye Kwon, Joonseog Kong, Sun Mi Moon, Sangsik Cho, Ki Young Yang, Won Il Jang, Mi Sook Kim, Younjoo Kim, Ui Sup Shin
Summary: Patient-derived tumor organoids closely resemble original patient tumors, and a machine learning algorithm using clinical and experimental radioresponse data has shown excellent performance in constructing a prediction model.
Article
Biology
Jeeyong Lee, Junhye Kwon, DaYeon Kim, Misun Park, KwangSeok Kim, InHwa Bae, Hyunkyung Kim, JoonSeog Kong, Younjoo Kim, UiSup Shin, EunJu Kim
Summary: The standard treatment for locally advanced rectal cancer (LARC) involves chemotherapy, radiotherapy, and surgery. Identifying biomarkers that can discriminate radio-responsiveness before surgery is crucial to improve treatment outcomes and reduce side effects. Research has identified candidate genes involved in radio-responsiveness, such as CTSE, which exhibited differential methylation at the promoter region inversely correlated with radio-resistance. This study aims to develop a gene chip for diagnosing radio-responsiveness in LARC patients, with the potential to improve patient prognosis.
Article
Biochemistry & Molecular Biology
Jeeyong Lee, Da Yeon Kim, Younjoo Kim, Ui Sup Shin, Kwang Seok Kim, Eun Ju Kim
Summary: To discover genes associated with radioresistance in colorectal cancer (CRC), an RNA sequencing dataset was analyzed. Among the candidates, IGFL2-AS1, a long non-coding RNA, showed a correlation with radioresistance. Further investigation revealed that as radioresistance increased, the expression of IGFL2-AS1 also increased in CRC cell lines and samples. Knockdown of IGFL2-AS1 decreased radioresistance and cell migration, indicating its functional role in radioresistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nursing
Sunjoo Kang, Younjoo Kim, Jinhyun Kim
Summary: This study aims to analyze the contribution of frontline nurses to the policy and law revision on infectious diseases in the Republic of Korea. The research found that nurses played an important role in the infectious disease response system, and their involvement led to significant policy changes. By establishing an epidemiological investigation team, Korean nurses demonstrated excellence in their capabilities and achieved positive outcomes in the fight against the COVID-19 pandemic.
JOURNAL OF NURSING SCHOLARSHIP
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Youn Joo Kim
Summary: Manufacturing a customized mastectomy bra using medical images is a viable alternative to anthropometric methods. Despite differences in measurement methods, the volume of the breast remains constant.
Article
Physiology
Younjoo Kim, Andrew Quach, Soumita Das, Kim E. Barrett
PHYSIOLOGICAL REPORTS
(2020)
Meeting Abstract
Gastroenterology & Hepatology
Younjoo Kim, Kim E. Barrett
Article
Law
Sunjoo Kang, Younjoo Kim, Insook Kim
JOURNAL OF EAST ASIA AND INTERNATIONAL LAW
(2017)
Meeting Abstract
Gastroenterology & Hepatology
Younjoo Kim, Jin Sun Shin, Ki Young Yang, Ui Sup Shin
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2016)