Review
Biochemistry & Molecular Biology
Ana Parra, Lorena Padilla, Xiomara Lucas, Heriberto Rodriguez-Martinez, Isabel Barranco, Jordi Roca
Summary: This systematic review focused on the relationship between seminal plasma extracellular vesicles (sEVs) and male (in)fertility. After screening and eligibility assessment, nine studies were selected, which identified differences in sEV molecules (specifically proteins and small non-coding RNAs) between fertile and subfertile/infertile males. The content of sEVs was also found to be related to sperm fertilizing capacity, embryo development, and implantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Amber Rose Hart, Norhayati Liaqat Ali Khan, Keerthie Dissanayake, Kasun Godakumara, Aneta Andronowska, Saji Eapen, Paul R. Heath, Alireza Fazeli
Summary: Successful embryo implantation and endometrial receptivity require communication between the embryo and the maternal endometrium, which is facilitated by extracellular vehicles (EVs). This study investigated the influence of menstrual cycle hormones (estrogen, progesterone, and estrogen plus progesterone) on endometrial-derived EVs. The results showed that hormonal stimulation affected the size and proteome of EVs, with proteins involved in embryo implantation, endometrial receptivity, and embryo development being enhanced in the EVs from the receptive phase (estrogen plus progesterone). This supports the concept of EV-mediated communication between the embryo and the maternal endometrium.
Article
Multidisciplinary Sciences
Kwadwo Asante-Afari, David Teye Doku, Eugene K. M. Darteh
Summary: This study explores the experiences of 40 women in Ghana who have successfully given birth through the use of assisted reproductive technologies. The findings reveal that childless women face hostility but having a child brings social recognition and transition to motherhood is characterized by excitement, high self-esteem, and acceptance into spouses' families.
Review
Endocrinology & Metabolism
Zhiqi Liao, Chang Liu, Lan Wang, Cong Sui, Hanwang Zhang
Summary: Reproductive disorders such as intrauterine adhesion, premature ovarian insufficiency, and polycystic ovary syndrome pose significant threats to female reproduction, but mesenchymal stem cells derived-extracellular vesicles have shown potentials in treating these diseases through various mechanisms.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jana Antalikova, Petra Secova, Katarina Michalkova, Lubica Horovska, Veronika Palenikova, Jana Jankovicova
Summary: This study reveals the localization and distribution of integrin aV in bull sperm, and suggests its interaction with tetraspanins CD9 and CD81. The findings contribute to understanding the role of integrins and tetraspanins in sperm development and maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Medicine, Research & Experimental
Xiaobo Liu, Jiajia Li, Wenjun Wang, Xue Ren, Ji-Fan Hu
Summary: Millions of women worldwide suffer from infertility associated with gynecologic disorders. Stem cell therapy has emerged as a new therapeutic option for the treatment of female reproductive and endocrine dysfunction. This review discusses the recent development and underlying mechanisms of stem cell therapy for a variety of female reproductive diseases.
Review
Multidisciplinary Sciences
Janaina Ferreira Aderaldo, Beatriz Helena Dantas Rodrigues de Albuquerque, Maryana Thalyta Ferreira Camara de Oliveira, Mychelle de Medeiros Garcia Torres, Daniel Carlos Ferreira Lanza
Summary: This study conducted a scoping review of the global assisted reproductive technologies (ART) market, identifying the main terms and categorizing them into different topics. The findings highlight the complexity of the market, with strong associations with economic, social, and geographic aspects. The study also emphasizes the need for further analysis of market elements related to technical improvements to enhance the success rate of in vitro fertilization (IVF).
Article
Medicine, General & Internal
Marta Correa Rancel, Elena Sosa Comino, Fatima Leon-Larios, Yaiza Suarez Hernandez, Janet Carballo Lorenzo, Diego Gomez-Baya, Delia Baez Quintana
Summary: During the pandemic, assisted reproductive treatments were disrupted, leading to increased anxiety levels in women whose treatments were suspended or delayed. Age pressure and worry were associated with higher levels of anxiety. Discontinuation of fertility treatments commonly resulted in feelings of sadness and anxiety.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Sevindzh Kletukhina, Guzel Mutallapova, Angelina Titova, Marina Gomzikova
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive disease caused by the aberrant regeneration of pulmonary epithelium. Mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) are promising therapies for pulmonary fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ling Wang, Shiyu Liu, Ka Li, Aijin Ma, Chenghu Hu, Changlin Wang, Nan Cao, Yunpeng Zhao, Ruifeng Fu, Wenwen Jia, Peng Xiang, Houqi Liu, Zhongquan Qi, Ningwen Zhu, Lingmin Liang, Lei Wang, Jiani Cao, Peijun Zhai, Jiaxi Zhou, Jun Wei, Tao Na, Jun Wu, Zhiying He, Guangdong Zhou, Weifeng Yu, Jinyan Wu, Wen Zeng, Yong Zhang, Lijun Zhu, Boqiang Fu, Jingzhong Zhang, Shuwei Yang, Chengxiang Dai, Hengmi Cui, Jianzhong Jing, Hexin Yan, Xiaowen He, Yongbo Lu, Cailing Tong, Tongbiao Zhao, Jie Hao, Xialin Liu, Yan Jin, Yue Wang
Summary: This article introduces the general requirements for the production of extracellular vesicles derived from human stem cells in China, aiming to promote standardization in research and production in this field and accelerate international standardization.
CELL PROLIFERATION
(2023)
Review
Endocrinology & Metabolism
Guannan Zhou, Yuanyuan Gu, Fangyue Zhou, Menglei Zhang, Ganrong Zhang, Ligang Wu, Keqin Hua, Jingxin Ding
Summary: Infertility is on the rise, and extracellular vesicles may play a crucial role in reproductive health, though the specific mechanism requires further investigation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Cell & Tissue Engineering
Senthilkumar Alagesan, Jack Brady, Declan Byrnes, Juan Fandino, Claire Masterson, Sean McCarthy, John Laffey, Daniel O'Toole
Summary: Cell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been explored for various diseases, especially those with inflammation. However, recent studies have shown that MSC is not a cure-all. This review discusses current and future strategies to overcome limitations in MSC efficacy, including inspirations from stem cell niche and previous gene therapy work. It also explores the emerging field of extracellular vesicle therapy and enhancement protocols.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Virinder Kaur Sarhadi, Ravindra Daddali, Riitta Seppanen-Kaijansinkko
Summary: Osteosarcoma is an aggressive bone tumor that predominantly affects children and adolescents, with a strong tendency to relapse and metastasize, posing challenges in identifying new therapeutic targets. Mesenchymal stem cells play a significant role in osteosarcoma development, contributing to tumor progression through intercellular signaling and communication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Yuling Huang, Lina Yang
Summary: MSC-EVs have demonstrated significant therapeutic effects on acute kidney injury, chronic kidney disease, diabetic nephropathy, and atherosclerotic renovascular disease, while their roles in transplanted kidneys remain controversial.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Shuqing Zhang, Cassidy Mulder, Suzette Riddle, Rui Song, Dongmei Yue
Summary: Bronchopulmonary dysplasia (BPD) is a common complication in preterm infants, and treatment options are limited. Recent studies have shown promising results in using mesenchymal stromal/stem cells (MSCs) to treat BPD, reducing inflammation, mitochondrial impairment, lung injury, and fibrosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Agriculture, Dairy & Animal Science
Ahmad Yar Qamar, Muhammad Ilyas Naveed, Sanan Raza, Xun Fang, Pantu Kumar Roy, Seonggyu Bang, Bereket Molla Tanga, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: Male fertility is negatively affected by endogenous stressors, such as reactive oxygen species (ROS), which reduce fertility. However, proper levels of ROS are necessary for sperm to complete various cellular functions. Excessive ROS production leads to oxidative stress (OS) and impairs sperm functions, resulting in male infertility. Numerous studies have shown that the use of natural and synthetic antioxidants can improve sperm quality-related parameters. This review focuses on the causes of OS, sources of ROS production, mechanisms of sperm damage, and the role of antioxidants in preserving sperm fertility.
Article
Genetics & Heredity
Islam M. M. Saadeldin, Bereket Molla Tanga, Seonggyu Bang, Abdulkadir Y. Y. Maigoro, Heejae Kang, Dabin Cha, Soojin Lee, Sanghoon Lee, Jongki Cho
Summary: This study identified 29 known mature miRNAs and 17 novel miRNAs in honeybee royal jelly extracellular vesicles (RJEVs). Further investigation showed that RJEVs can reduce apoptosis and enhance cell vitality and healing ability. The findings suggest that RJEVs regulate gene expression to maintain cell survival and promote cell regeneration.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Oncology
Xun Fang, Seonggyu Bang, Bereket Molla Tanga, Chaerim Seo, Dongjie Zhou, Gyeonghwan Seong, Islam M. Saadeldin, Sanghoon Lee, Xiang-Shun Cui, Jongki Cho
Summary: Assisted reproductive technology has improved animal reproduction efficiency. However, polyspermy is a limitation in porcine IVF. This study investigated the effects of porcine oviduct epithelial cells (OEC-EVs) on sperm-oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. The results showed that treatment with OEC-EVs significantly increased cleavage rate, number of embryos, and decreased polyspermy rate compared to the control group. Additionally, OEC-EVs improved cortical granule concentration and distribution, increased oocyte mitochondrial activity, and increased the IVF success rate.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Veterinary Sciences
Hani A. Ba-Awadh, Isiaka O. Olarinre, Abdullah N. Alowaimer, Islam M. Saadeldin, Ayman A. Swelum
Summary: This study aimed to compare two freezing protocols for Najdi ram's semen. The rapid freezing protocol showed better post-thawing semen quality compared to the slow freezing protocol, including higher vitality, fast progressive motility, straight-line velocity, average pathway velocity, linearity, and wobble.
SLOVENIAN VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: Combining rolipram and cilostamide as cAMP modulators can greatly enhance the developmental competence of in vitro matured porcine oocytes. Pre-treatment for four hours was found to maintain optimal gap junction communication. The combined treatment group showed higher glutathione levels, lower reactive oxygen species levels, and a higher maturation rate compared to the control and single treatment groups. Two-phase in vitro maturation resulted in higher cleavage and blastocyst formation rates in parthenogenetic activation and somatic cell nuclear transfer embryos.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Islam M. Saadeldin, Bereket Molla Tanga, Seonggyu Bang, Chaerim Seo, Abdulkadir Y. Maigoro, Heejae Kang, Dabin Cha, Sung Ho Yun, Seung Il Kim, Sanghoon Lee, Jongki Cho
Summary: This study successfully isolated, characterized, and utilized egg yolk nanovesicles (VVs) as a supplement for porcine embryo culture. The results showed that VVs enhanced embryonic cell count, reduced apoptosis, and promoted embryo attachment and embryonic stem cell outgrowth. These effects may be attributed to the protein and miRNA cargo contents of VVs, providing a foundation for further research on the application of egg yolk nanovesicles in human embryo culture.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Islam M. Saadeldin, Seonggyu Bang, Abdulkadir Y. Maigoro, Sung Ho Yun, Seung Kim, Sanghoon Lee, Jongki Cho
Summary: This study isolated and characterized nanovesicles from mature porcine oocytes, which were named as intra-ooplasmic vesicles (IOVs) for the first time. Proteomic analysis revealed that these IOVs contained proteins related to reprogramming, antioxidative defense, cytoskeleton, heat shock proteins, and metabolism. Supplementing cultured fibroblasts with IOVs resulted in changes in cell morphology and the expression of pluripotency and trophoblastic markers.
CELLULAR REPROGRAMMING
(2023)
Retraction
Multidisciplinary Sciences
Bereket Molla Tanga, Seonggyu Bang, Xun Fang, Chaerim Seo, Mahanama De Zoysa, Islam M. Saadeldin, Sanghoon Lee, Sang Un Park, Sun-Ok Chung, Geung-Joo Lee, Jongki Cho
Article
Agriculture, Dairy & Animal Science
Jihyun Park, Wonyou Lee, Islam M. Saadeldin, Seonggyu Bang, Sanghoon Lee, Junkoo Yi, Jongki Cho
Summary: This study aimed to assess the effects of embryonic developmental stage, quality grade, and fresh or frozen/thawed conditions on the pregnancy rate and sex ratio of live offspring in Hanwoo cows. The results showed that embryo quality, developmental stage, and cryopreservation significantly affected the pregnancy rate, but there was no significant difference in pregnancy rates between fresh and frozen/thawed embryos.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
