Article
Biochemistry & Molecular Biology
Lavanya Moparthi, Stefan Koch
Summary: The Wnt/fl-catenin pathway is regulated by multiple FOX transcription factors, which play a crucial role in development and stem cell maintenance.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Developmental Biology
Gianluca Zambanini, Anna Nordin, Mattias Jonasson, Pierfrancesco Pagella, Claudio Cantu
Summary: We developed a modified version of CUT&RUN called LoV-U, which allows for stable and reproducible generation of beta-catenin binding profiles, uncovering its direct target genes in human cells and mouse tissues. This method outperforms the original CUT&RUN when targeting co-factors that do not bind DNA, and is suitable for processing multiple samples simultaneously.
Article
Biochemistry & Molecular Biology
Zhaobo Cheng, Renjie Yu, Li Li, Junhao Mu, Yijia Gong, Fan Wu, Yujia Liu, Xiangyi Zhou, Xiaohua Zeng, Yongzhong Wu, Ran Sun, Tingxiu Xiang
Summary: In our research, we identified ZNF334 as a novel tumor suppressor of triple-negative breast cancer (TNBC). ZNF334 expression was usually reduced in breast cancer tissues and TNBC cell lines, likely due to promoter hypermethylation. Ectopic expression of ZNF334 suppressed the growth and metastatic capacity of TNBC cell lines, induced cell cycle arrest at S phase and apoptosis. Additionally, re-expression of ZNF334 restored the Epithelial-Mesenchymal Transition (EMT) process and inhibited stemness by up-regulating SFRP1.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yoshifumi Takahata, Hiromasa Hagino, Ayaka Kimura, Mitsuki Urushizaki, Shiori Yamamoto, Kanta Wakamori, Tomohiko Murakami, Kenji Hata, Riko Nishimura
Summary: This review article summarizes the research progress on the functions and regulatory mechanisms of lubricin and growth differentiation factor 5 (Gdf5) in osteoarthritis (OA), as well as their roles in maintaining articular cartilage homeostasis and the development of OA. Molecular targeting of Prg4 and Gdf5 is expected to provide new insights into the etiology, pathogenesis, and potential treatment of OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Hazel M. Quinn, Regina Vogel, Oliver Popp, Philipp Mertins, Linxiang Lan, Clemens Messerschmidt, Alexandro Landshammer, Kamil Lisek, Sophie Chateau-Joubert, Elisabetta Marangoni, Elle Koren, Yaron Fuchs, Walter Birchmeier
Summary: The research reveals that in basal-like breast cancers, YAP signaling is essential for beta-catenin activity, controlling the cancer stem cell (CSC) program. Targeting the YAP/TEAD4/beta-catenin complex may offer a potential therapeutic strategy for eradicating CSCs in basal-like breast cancers.
Article
Cell Biology
Bin Shang, Fengjuan Lu, Shujuan Jiang, Mengmeng Xing, Xinyu Mao, Guanghai Yang, Hao Zhang
Summary: This study found that ALDOC protein was overexpressed in non-small cell lung cancer (NSCLC) tissues and was associated with lymph node metastasis and pathological stage. ALDOC promoted NSCLC by affecting MYC-mediated UBE2N transcription and regulating the Wnt pathway. Downregulation of UBE2N or the use of Wnt pathway inhibitor could reverse the promoting effects of ALDOC elevation on NSCLC development.
Article
Biochemistry & Molecular Biology
Byeong-Yun Ahn, Myong-Ho Jeong, Jung-Hoon Pyun, Hyeon-Ju Jeong, Tuan Anh Vuong, Ju-Hyeon Bae, Subin An, Su Woo Kim, Yong Kee Kim, Dongryeol Ryu, Hyun-Ji Kim, Hana Cho, Gyu-Un Bae, Jong-Sun Kang
Summary: PRMT7 plays an important role in cardiac hypertrophy and fibrosis. Overexpression of PRMT7 can alleviate cellular hypertrophic response, while depletion of PRMT7 exacerbates hypertrophic response. Transcriptome analysis reveals altered gene expression profile related to Wnt signaling pathway in PRMT7-deficient hearts.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
German Belenguer, Gianmarco Mastrogiovanni, Clare Pacini, Zoe Hall, Anna M. Dowbaj, Robert Arnes-Benito, Aleksandra Sljukic, Nicole Prior, Sofia Kakava, Charles R. Bradshaw, Susan Davies, Michele Vacca, Kourosh Saeb-Parsy, Bon-Kyoung Koo, Meritxell Huch
Summary: RNF43/ZNRF3 alterations contribute to liver disease and liver cancer by affecting differentiation and lipid metabolism of hepatocytes. The loss of these genes leads to steatohepatitis and an increase in unsaturated lipids. Deletion of Rnf43/Znrf3 results in defective hepatocyte regeneration and liver cancer, causing an imbalance between differentiation and proliferation. Interestingly, ZNRF3 mutant liver cancer patients have a poorer prognosis and exhibit altered hepatic lipid metabolism and steatohepatitis/NASH signatures.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Han Song, Junjun Qiu, Keqin Hua
Summary: USP14 is overexpressed in cervical cancer and is associated with clinical stage and prognosis of patients. It promotes the proliferation, migration, and invasion of cervical cancer cells by upregulating the expression of beta-catenin.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Oncology
Anil Belur Nagaraj, Matthew Knarr, Sreeja Sekhar, R. Shae Connor, Peronne Joseph, Olga Kovalenko, Alexis Fleming, Arshia Surti, Elmar Nurmemmedov, Luca Beltrame, Sergio Marchini, Michael Kahn, Analisa DiFeo
Summary: Wnt signaling plays a critical role in ovarian cancer, and miR-181a has been identified as a novel driver of the Wnt/β-catenin signaling pathway, promoting stemness and chemoresistance. Targeting the miR-181a-SFRP4 axis may offer a potential therapeutic strategy for HGSOC.
Article
Biochemistry & Molecular Biology
Maria Abancens, Brian J. Harvey, Jean McBryan
Summary: Women have lower incidence and mortality rates for colorectal cancer compared to men. Estrogen may protect against colorectal cancer progression through GPER-mediated modulation of the Wnt/beta-catenin pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Yutao Li, Shanhe Wang, Wei Sun
Summary: SRY-box transcription factor 18 (SOX18) is expressed in dermal papilla cells (DPCs) of sheep hair follicles and plays a crucial role in promoting DPC proliferation through activating the Wnt/β-Catenin signaling pathway. Inhibition of the Wnt/β-Catenin signaling pathway can be rescued by SOX18, suggesting the essential role of SOX18 in governing DPC proliferation and hair growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Stefan Koch, Sergio P. Acebron, Jessica Herbst, Gencay Hatiboglu, Christof Niehrs
Article
Developmental Biology
Leila R. Martins, Raffaela K. Bung, Stefan Koch, Karsten Richter, Laura Schwarzmueller, Dorothee Terhardt, Bahtiyar Kurtulmus, Christof Niehrs, Arefeh Rouhi, Ingrid Lohmann, Gislene Pereira, Stefan Froehling, Hanno Glimm, Claudia Scholl
DEVELOPMENTAL BIOLOGY
(2018)
Article
Pathology
Philipp-Alexander Neumann, Stefan Koch, Roland S. Hilgarth, Ernesto Perez-Chanona, Patricia Denning, Christian Jobin, Asma Nusrat
AMERICAN JOURNAL OF PATHOLOGY
(2014)
Article
Immunology
Manirath Khounlotham, Wooki Kim, Eric Peatman, Porfirio Nava, Oscar Medina-Contreras, Caroline Addis, Stefan Koch, Benedicte Fournier, Asma Nusrat, Timothy L. Denning, Charles A. Parkos
Meeting Abstract
Gastroenterology & Hepatology
Porfirio Nava, Ryuta Kamekura, Oscar Medina-Contreras, Ross Hamilton, Keli Kolegraff, Stefan Koch, Timothy Denning, Charkes Parkos, Asma Nusrat
INFLAMMATORY BOWEL DISEASES
(2012)
Review
Cell Biology
Lavanya Moparthi, Stefan Koch
Article
Multidisciplinary Sciences
Lavanya Moparthi, Giulia Pizzolato, Stefan Koch
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Gastroenterology & Hepatology
Celia Escudero-Hernandez, Andreas Munch, Ann-Elisabet Ostvik, Atle van Beelen Granlund, Stefan Koch
JOURNAL OF CROHNS & COLITIS
(2020)
Article
Cell Biology
Lavanya Moparthi, Stefan Koch
Article
Cell Biology
Andrea Molinas, Stephanie Heil, Stefan Koch
Summary: While the CCNY gene has been implicated in the pathogenesis of inflammatory bowel disease, a study investigating the role of cyclin Y in intestinal epithelial cells found that its absence did not significantly affect cell proliferation or disease activity. The low expression of cyclin-Y-associated CDKs in IECs suggests that cyclin Y may not play a crucial role in intestinal epithelial homeostasis, casting doubt on CCNY mutations as causative factors in IBD.
Article
Cell Biology
Giulia Pizzolato, Lavanya Moparthi, Simon Soderholm, Claudio Cantu, Stefan Koch
Summary: The forkhead box transcription factor FOXQ1 plays a crucial role in the pathogenesis of colorectal cancers by promoting tumour metastasis and activating the Wnt/beta-catenin signalling pathway. FOXQ1 enhances Wnt-induced transcription and regulates the expression of beta-catenin target genes. It also controls the expression of various Wnt target genes independently of beta-catenin. Furthermore, FOXQ1 is involved in a transcriptional program linked to EMT and cell migration, and interacts with similar co-factors as the beta-catenin-associated transcription factor Tcf711.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Stefan Koch
Article
Cell Biology
Nadine Kirsch, Ling-Shih Chang, Stefan Koch, Andrey Glinka, Christine Dolde, Gabriele Colozza, Maria D. J. Benitez, Edward M. De Robertis, Christof Niehrs
DEVELOPMENTAL CELL
(2017)
Article
Biochemistry & Molecular Biology
Birgit S. Berger, Sergio P. Acebron, Jessica Herbst, Stefan Koch, Christof Niehrs