Article
Biochemistry & Molecular Biology
Hitoshi Iwamoto, Masaaki Okihara, Isao Akashi, Yu Kihara, Osamu Konno, Shigeyuki Kawachi, Makoto Sunamura, Masahiro Sugimoto
Summary: Kidney biopsy is commonly used to diagnose kidney transplant dysfunction. In this study, capillary electrophoresis-mass spectrometry was used to analyze the biofluids of transplant recipients. Metabolomics analyses revealed significant differences in the metabolomic profiles of the different groups, particularly in arginine biosynthesis and purine metabolism in plasma samples. Different types of kidney disorders also showed distinct metabolomic profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Rintaro Saito, Masahiro Sugimoto, Akiyoshi Hirayama, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: Large-scale metabolomic studies are common, but there is less focus on the reliability of uncharacterized peaks in untargeted metabolomics data. This study tested criteria to assess the reliability of uncharacterized peaks and identified some reliable peaks potentially related to important physiological processes like disease progression.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Analytical
Kevin Jooss, Luis F. Schachner, Rachel Watson, Zachary B. Gillespie, Sarah A. Howard, Marcus A. Cheek, Matthew J. Meiners, Amin Sobh, Jonathan D. Licht, Michael-Christopher Keogh, Neil L. Kelleher
Summary: The study introduces a new platform for separating and characterizing whole nucleosomes and their modifications, aiding in understanding epigenetic regulatory mechanisms. The platform offers low sample requirements and costs, with high detection sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Kaori Igarashi, Sana Ota, Miku Kaneko, Akiyoshi Hirayama, Masanobu Enomoto, Kenji Katumata, Masahiro Sugimoto, Tomoyoshi Soga
Summary: A high-throughput polyamine metabolite profiling method based on multisegment injection capillary electrophoresis triple quadrupole tandem mass spectrometry was developed in this study. The method is sensitive, selective, and quantitative, allowing for rapid screening of polyamines in a large number of samples and discrimination of disease patients.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Qinlei Liu, Sandra Martinez-Jarquin, Wenjie Ge, Renato Zenobi
Summary: Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susanna Della Posta, Chiara Fanali, Valeria Gallo, Salvatore Fanali
Summary: This paper presents recent results obtained by coupling electromigration techniques with mass spectrometry, highlighting the advantages of this hybrid approach and discussing selected applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yoji Saeki, Akane Takenouchi, Etsuyo Otani, Minji Kim, Yumi Aizawa, Yasuko Aita, Atsumi Tomita, Masahiro Sugimoto, Takashi Matsukubo
Summary: This study evaluated the effect of long-term mastication on salivary metabolomic profiles and found that most metabolites showed lower concentrations after chewing. The pathways related to glycogenic amino acids also underwent significant changes.
Article
Biochemical Research Methods
Hongying Zhu, Qianqian Li, Tiepeng Liao, Xiang Yin, Qi Chen, Ziyi Wang, Meifang Dai, Lin Yi, Siyuan Ge, Chenjian Miao, Wenping Zeng, Lili Qu, Zhenyu Ju, Guangming Huang, Chunlei Cang, Wei Xiong
Summary: SLMS integrates lysosomal patch-clamp recording and induced nanoESI/MS for concurrent metabolic and electrophysiological profiling of individual enlarged lysosomes. This technique has been validated for accuracy and reliability, enabling the classification of lysosomes into subpopulations based on their chemical and biological differences in various cell types. SLMS has the potential to investigate heterogeneous lysosomal metabolic changes during physiological and pathological processes.
Article
Biochemical Research Methods
Monika Skrutkova Langmajerova, Marta Pelcova, Pavla Vedrova, Andrea Cela, Zdenek Glatz
Summary: This study presents a method for amino acid determination in a limited volume sample collected after in vitro fertilization, achieving successful separation and quantification of 19 amino acids through optimized sample preparation and key parameters. The developed system has the potential for analyzing amino acids in minute sample volumes of other biological matrices.
Article
Chemistry, Analytical
Radim Polasek, Klara Konderlova, Jan Petr
Summary: The aim of this study was to develop a new method for analyzing tamsulosin enantiomers using capillary electrophoresis combined with tandem mass spectrometry. The method achieved a limit of detection of 1.6 nmol L-1 under optimal conditions. It was validated for linearity, detection and quantification limits, precision, recovery, and selectivity, and can be applied to the analysis of tamsulosin enantiomers in environmental samples and other analytical tasks.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Bo Liu, Wentao Wang, Tie Gao, Lu Huang, Huihong Fan, Hong-Xu Chen
Summary: Cobratide, a peptide drug extracted from Chinese cobra venom, has shown potential in treating not only chronic pain but also COVID-19. A protocol combining sheathless CE-MS and CE-UV methods was established for the separation, identification, and quantification of cobratide and its impurities, providing valuable information for quality control and process analysis of peptide drugs.
ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Maria Castro-Puyana, Maria L. Marina
Summary: This review article critically examines the significant advances in using CE-MS-based approaches to assess various aspects of Food Science, and discusses the latest developments in hardware and software.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Oncology
Tzu-Rong Peng, Hung-Hong Lin, Li-Jou Yang, Ta-Wei Wu
Summary: This meta-analysis showed that compared with placebo, glutamine significantly reduced the incidence of grade 3 and 4 oral mucositis induced by chemotherapy or radiation therapy. Oral administration of glutamine and the use of medium or low doses can decrease the risk. Glutamine also had a significant effect in reducing the risk of grade 3 and 4 oral mucositis induced by radiotherapy, especially in prevention.
SUPPORTIVE CARE IN CANCER
(2021)
Article
Oncology
Alessandro Villa, Michele Vollemans, Amanda De Moraes, Stephen Sonis
Summary: This study evaluated the concordance of three commonly used oral mucositis grading criteria, finding relatively high levels of consistency in all OM scores and severe OM scores. This indicates that these grading criteria have high accuracy in assessing oral mucositis.
SUPPORTIVE CARE IN CANCER
(2021)
Article
Oncology
Takao Ueno, Wakako Yatsuoka, Hiroto Ishiki, Kanako Miyano, Yasuhito Uezono
Summary: The results of this prospective study showed that Episil (R) could be an effective treatment to relieve oral pain in Japanese patients with moderate to severe OM, and this newly approved product might adequately support patients' oral intake.
Article
Multidisciplinary Sciences
Kanako Miyano, Minori Ikehata, Kaori Ohshima, Yuki Yoshida, Yasuhiro Nose, Sei-ichi Yoshihara, Katsuyuki Oki, Seiji Shiraishi, Miaki Uzu, Miki Nonaka, Yoshikazu Higami, Yasuhito Uezono
Summary: AD-MSCs and UC-MSCs have therapeutic potential in neuropathic pain and may help in the recovery through different regulatory mechanisms.
Article
Pharmacology & Pharmacy
Kanako Miyano, Seiya Hasegawa, Noriho Asai, Miaki Uzu, Wakako Yatsuoka, Takao Ueno, Miki Nonaka, Hideaki Fujii, Yasuhito Uezono
Summary: The Japanese herbal medicine Hangeshashinto (HST) has been shown to improve chemotherapy-induced oral ulcerative mucositis (OUM) through enhancing the migration of human oral keratinocytes (HOK) by upregulating CXCL12 via ERK.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Anesthesiology
Akane Komatsu, Kanako Miyano, Daisuke Nakayama, Yusuke Mizobuchi, Eiko Uezono, Kaori Ohshima, Yusuke Karasawa, Yui Kuroda, Miki Nonaka, Keisuke Yamaguchi, Masako Iseki, Yasuhito Uezono, Masakazu Hayashida
Summary: The compounds N1 and N2 have high transdermal absorbability and equivalent analgesic effects as morphine. They may be attractive compounds for the development of novel opioid patches for transitioning from fentanyl patches.
ANESTHESIA AND ANALGESIA
(2022)
Review
Biochemistry & Molecular Biology
Serafettin Gunes, Yumi Aizawa, Takuma Sugashi, Masahiro Sugimoto, Pedro Pereira Rodrigues
Summary: Early diagnosis is crucial for delaying the onset of Alzheimer's disease, and current diagnostic methods are invasive and expensive, highlighting the need for more convenient and effective biomarkers. Studies have identified potential biomarkers, but further validation research is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yoji Saeki, Akane Takenouchi, Etsuyo Otani, Minji Kim, Yumi Aizawa, Yasuko Aita, Atsumi Tomita, Masahiro Sugimoto, Takashi Matsukubo
Summary: This study evaluated the effect of long-term mastication on salivary metabolomic profiles and found that most metabolites showed lower concentrations after chewing. The pathways related to glycogenic amino acids also underwent significant changes.
Article
Gastroenterology & Hepatology
Taisuke Matsumoto, Masakatsu Fukuzawa, Takao Itoi, Masahiro Sugimoto, Yumi Aizawa, Makoto Sunamura, Takashi Kawai, Daiki Nemoto, Hirokazu Shinohara, Takahiro Muramatsu, Yuka Suzuki, Yasuyuki Kagawa, Maya Suguro, Kumiko Uchida, Yohei Koyama, Akira Madarame, Takashi Morise, Hayato Yamaguchi, Akihiko Sugimoto, Yoshiya Yamauchi, Shin Kono, Sakiko Naito
Summary: This study analyzed plasma metabolomics and identified a combination of quantified metabolites that showed high sensitivity for the detection of gastric cancer (GC). These metabolites were found to be independent of commonly used tumor markers, suggesting that their complementary use could be helpful for GC screening.
Article
Biochemistry & Molecular Biology
Daisuke Nose, Masahiro Sugimoto, Tsuneo Muta, Shin-Ichiro Miura
Summary: Pancreatic cancer is a highly malignant cancer with a poor prognosis. It is often diagnosed late due to the absence of typical symptoms. Therefore, it is necessary to establish a screening method for early detection of pancreatic cancer in high-risk individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Yoshie Nakayama, Mariko Hanada, Hiroshi Koda, Masahiro Sugimoto, Masahiro Takada, Masakazu Toi
Summary: This study aimed to evaluate volatile compounds in exhaled breath as a non-invasive screening method to detect breast neoplasms. After analyzing breath samples, significant differences in volatile compounds were found between patients with breast cancer and non-breast cancer controls. The developed MLR model can effectively discriminate between breast cancer and non-breast cancer samples. This study suggests the potential of volatile compounds in exhaled breath as a noninvasive screening method for breast cancer.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Akiyoshi Hirayama, Takamasa Ishikawa, Haruka Takahashi, Sanae Yamanaka, Satsuki Ikeda, Aya Hirata, Sei Harada, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: High-throughput metabolomics has facilitated large-scale cohort studies. Long-term studies require multiple batch-based measurements, which necessitates sophisticated quality control to eliminate bias. In this study, liquid chromatography-mass spectrometry was used to analyze 10,833 samples in 279 batch measurements. The quantified profile included 147 lipids. Normalization using QC samples reduced the coefficient of variation for the lipids. This study provides unbiased and quantified data for large-scale metabolomics.
Article
Oncology
Sachika Shiraishi, Masahiro Sugimoto, Koichi Tokuuye
Summary: The study evaluated salivary metabolomics as an independent predictor for radiation pneumonitis, identifying three metabolites with predictive ability for clinical RP.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Medicinal
Hiroshi Arakawa, Takumi Kawanishi, Dai Shengyu, Takumi Nishiuchi, Makiko Meguro-Horike, Shin-ichi Horike, Masahiro Sugimoto, Yukio Kato
Summary: This study investigates the effects of hepatobiliary cholestasis on renal exposure to nephrotoxic drugs. The results show that tissue accumulation of imatinib and cisplatin in kidney slices is higher in mice with bile duct ligation (BDL) compared to sham-operated mice. Proteomic analysis reveals a reduction in the expression of the efflux transporter Mrp6 in the kidneys of BDL mice. These findings suggest that cholestasis leads to drug accumulation in renal cells and promotes drug-induced renal injury.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Daisuke Nose, Tomokazu Matsui, Takuya Otsuka, Yuki Matsuda, Tadaaki Arimura, Keiichi Yasumoto, Masahiro Sugimoto, Shin-Ichiro Miura
Summary: A study developed a model to estimate pleural effusion in heart failure patients using multi-frequency bioelectrical impedance analysis, which showed high accuracy in discriminating patients with pleural effusion. This approach offers a potential clinical application for assessing intrathoracic conditions.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Health Care Sciences & Services
Masahiro Sugimoto, Atsumi Tomita, Michiko Oyamada, Mitsue Sato
Summary: The gaze trajectories of nurses with different levels of experience were monitored during an intravenous injection task. The results showed that experienced nurses had different patterns of gaze duration compared to nursing students. A state transition diagram visualized the recognition errors and repeated confirmation behaviors. Clustering analysis of gaze durations also indicated similarity among participants with similar experiences.
Article
Multidisciplinary Sciences
Misa Yoshizawa, Masahiro Sugimoto, Minoru Tanaka, Yusuyuki Sakai, Masaki Nishikawa
Summary: Liver fibrosis is a result of homeostasis breakdown caused by repetitive injury, leading to collagen accumulation that disrupts liver structure and function, resulting in serious consequences such as cirrhosis. Study using mathematical simulation model found accumulation of collagens from different sources in different regions of the liver, providing insights into a new mechanism for understanding liver fibrosis.
SCIENTIFIC REPORTS
(2022)
