期刊
ACS SENSORS
卷 6, 期 7, 页码 2654-2663出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c00607
关键词
selective plane illumination microscopy; light-sheet microscopy; three-dimensional imaging; optical sectioning; bacteria detection; particle detection; fluorescence fluctuation spectroscopy; image correlation spectroscopy
资金
- National Institute of General Medical Sciences of the National Institutes of Health [P41GM103540, R21GM135493]
- UCI
miniSPIM is a miniaturized light-sheet microscope that allows optical sectioning on mobile camera devices like smartphones and single-board computers, making it portable, low-power, and cost-effective. It has applications in biosensing, field research, and education, enabling various imaging tasks in a compact size.
The miniSPIM is a miniaturized light-sheet microscope that enables imaging with optical sectioning on mobile camera devices such as smartphones and single-board computers. Applications of the miniSPIM include biosensing, field research, and education where maximum portability and robustness, low power consumption, and low cost are key. Here, it is shown how all of the components of a simple light-sheet microscope can be integrated within a footprint smaller than the average smartphone. Example applications include the quantification of the motion of microparticles and bacteria in fluids, the characterization of solvent polarity based on spectral shifts of the lipid probe Nile Red, and three-dimensional (3D) and time-lapse autofluorescence imaging of a live zebrafish embryo.
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