Reducing antigenicity of β-lactoglobulin, probiotic properties and safety evaluation of Lactobacillus plantarum AHQ-14 and Lactobacillus bulgaricus BD0390

Probiotics are important bacteria because they are beneficial to human health. Two lactic acid bacteria (LAB) strains capable to hydrolyze immuno-reactive beta-lactoglobulin (beta-Lg) proteins in milk, including AHQ-14 isolated from Chinese Xinjiang district traditional dairy products and identified as Lactobacillus plantarum by 16S rRNA gene sequences analysis, and Lactobacillus bulgaricusBD0390 kindly donated by Bright Dairy Company (Shanghai, China), were investigated for their probiotic traits and safety. Lactobacillus plantarumAHQ-14 and Lactobacillus bulgaricusBD0390 were tested their ability to lower beta-Lg antigenicity, bile salt hydrolase (BSH) activity, survival rate in gastrointestinal and adhesion capacity to HT-29 cells. For preliminary safety assessment, L.plantarumAHQ-14 and L.bulgaricusBD0390 were tested for hemolytic activity, biogenic amines (BAs) production and for their antibiotic resistance. Results showed that L.plantarumAHQ-14 and L.bulgaricusBD0390 were gamma-hemolytic and showed high abilities to adhere to HT-29 cells. They exhibited higher survival rates than the control (Lactobacillus rhamnosusGG) under simulated gastric conditions. Both strains produced a small amount of BAs, similar to or even lower than the BAs content in the regularly consumed foods. Furthermore, they showed sensitivity towards conventionally used antibiotics. In conclusion, L.plantarumAHQ-14 and L.bulgaricusBD0390 possessed good characteristics as potential probiotic for functional fermented food in the future.

Automated summary

The study showed that L.plantarum AHQ-14 and L.bulgaricus BD0390 exhibited good probiotic characteristics, such as high survival rates, strong adhesion capacity, low production of biogenic amines, and sensitivity to antibiotics.