Identification of tomato root growth regulatory genes and transcription factors through comparative transcriptomic profiling of different tissues

Tomato is an economically important vegetable crop and a model for development and stress response studies. Although studied extensively for understanding fruit ripening and pathogen responses, its role as a model for root development remains less explored. In this study, an Illumina-based comparative differential transcriptomic analysis of tomato root with different aerial tissues was carried out to identify genes that are predominantly expressed during root growth. Sequential comparisons revealed similar to 15,000 commonly expressed genes and similar to 3000 genes of several classes that were mainly expressed or regulated in roots. These included 1069 transcription factors (TFs) of which 100 were differentially regulated. Prominent amongst these were members of families encoding Zn finger, MYB, ARM, bHLH, AP2/ERF, WRKY and NAC proteins. A large number of kinases, phosphatases and F-box proteins were also expressed in the root transcriptome. The major hormones regulating root growth were represented by the auxin, ethylene, JA, ABA and GA pathways with root-specific expression of certain components. Genes encoding carbon metabolism and photosynthetic components showed reduced expression while several protease inhibitors were amongst the most highly expressed. Overall, the study sheds light on genes governing root growth in tomato and provides a resource for manipulation of root growth for plant improvement.

Automated summary

This study utilized comparative differential transcriptomic analysis to identify genes predominantly expressed during root growth in tomato, revealing common expression of around 15,000 genes and root-specific expression of approximately 3000 genes of various classes. Root-specific expression was observed for numerous transcription factors, kinases, phosphatases, and hormone pathways related to root growth.