Article
Biochemistry & Molecular Biology
Vita Golubovskaya, Hua Zhou, Feng Li, Robert Berahovich, Jinying Sun, Michael Valentine, Shirley Xu, Hizkia Harto, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: This study focused on developing novel CS1 CAR-T cells and bispecific CS1-BCMA CAR-T cells for targeting multiple myeloma. The experimental results demonstrated that these cells effectively killed multiple myeloma cells, showing promise for future clinical trials.
Article
Chemistry, Multidisciplinary
Valeria Ukrainskaya, Yuri Rubtsov, Dmitry Pershin, Nadezhda Podoplelova, Stanislav Terekhov, Igor Yaroshevich, Anstasiia Sokolova, Dmitry Bagrov, Elena Kulakovskaya, Victoria Shipunova, Sergey Deyev, Rustam Ziganshin, Aleksandr Chernov, Georgii Telegin, Eugene Maksimov, Oleg Markov, Anastasiya Oshchepkova, Marina Zenkova, Jia Xie, Hongkai Zhang, Alexander Gabibov, Michael Maschan, Alexey Stepanov, Richard Lerner
Summary: The development of CAR-T therapy has achieved success in the treatment of B cell leukemia, but the expansion of CAR-T cells remains challenging with current protocols. A novel approach using cell-derived membrane vesicles has been proposed, showing greater potential in stimulating, proliferating, and enhancing the functional activity of CAR-T cells.
Article
Oncology
Huanpeng Chen, Yuying Yang, Yuqing Deng, Fengjiao Wei, Qingyu Zhao, Yongqi Liu, Zhonghua Liu, Bolan Yu, Zhaofeng Huang
Summary: This study developed CAR-T cells that can secrete a CD47 blocker, enhancing the therapeutic efficacy of CAR-T cells in solid tumor therapy. The results showed that these CAR-T cells significantly reduced tumor burden, prolonged survival, and modulated the tumor microenvironment.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Celia Martin-Otal, Aritz Lasarte-Cia, Diego Serrano, Noelia Casares, Enrique Conde, Flor Navarro, Ines Sanchez-Moreno, Marta Gorraiz, Patricia Sarrion, Alfonso Calvo, Carlos E. De Andrea, Jose Echeveste, Amaia Vilas, Juan Roberto Rodriguez-Madoz, Jesus San Miguel, Felipe Prosper, Sandra Hervas-Stubbs, Juan Jose Lasarte, Teresa Lozano
Summary: This study demonstrates the feasibility and efficacy of targeting the tumor-specific fibronectin splice variant EDA with CAR-T cells, providing a potential therapeutic option for different types of cancer.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Nanoscience & Nanotechnology
Yuanyuan Zhang, Tong Ge, Meijuan Huang, Yun Qin, Tianjiao Liu, Wei Mu, Gaoxiang Wang, Lijun Jiang, Tongjuan Li, Lei Zhao, Jue Wang
Summary: This study investigated the potential of CAR antigen-modified extracellular vesicles (EVs) to enhance the effectiveness of CAR-T cell therapy. The results showed that CD19 antigen-expressing EVs specifically and dose-dependently activated CAR-T cells, leading to selective expansion and cytokine secretion. Furthermore, the CD19 EVs increased the anti-tumor activity of CAR-T cells. In vivo experiments demonstrated that CD19 EVs promoted the functional persistence of CAR-T cells. Overall, these findings suggest that antigen-expressing EVs can be utilized as a boost to improve the efficacy of CAR-T cell therapy.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Vita Golubovskaya
Summary: CAR-T cell therapy is a promising immunotherapy for hematological cancers. This report discusses the targeting of immune checkpoint proteins by CAR-T cells, highlighting future directions and perspectives.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Biochemistry & Molecular Biology
Zoufang Huang, Saikat Dewanjee, Pratik Chakraborty, Niraj Kumar Jha, Abhijit Dey, Moumita Gangopadhyay, Xuan-Yu Chen, Jian Wang, Saurabh Kumar Jha
Summary: Malignant brain tumors are difficult to manage, but there is optimism that immunotherapy using CAR T cells may help. CAR T cells use tumor-targeting specificity to direct T cells' cytolytic capacity. Several molecules have been identified as potential targets for immunotherapy in brain cancer. However, there are factors that slow down the progress of CAR T cell treatments for brain cancers. This study offers a thorough analysis of CAR T cells' promise in treating brain cancer and potential solutions to the issues encountered.
Review
Immunology
Ya-Ting Qin, Ya-Ping Li, Xi-Wen He, Xi Wang, Wen-You Li, Yu-Kui Zhang
Summary: Chimeric antigen receptor-T (CAR-T) cell therapy is facing challenges due to complex manufacturing processes, high costs, and disappointing results in treating solid tumors. However, the fusion of immunology, cell biology, and biomaterials has provided new strategies to overcome these obstacles. Biomaterials-assisted CAR-T engineering has improved therapeutic efficacy and minimized side effects, offering a sustainable approach for cancer immunotherapy. This review summarizes the role of biomaterials in gene delivery for CAR-T cell generation and highlights their potential in synergistic immunotherapy for solid tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Jingwen Shao, Lin Hou, Jinyan Liu, Yulin Liu, Jie Ning, Qitai Zhao, Yi Zhang
Summary: In this study, the role of indoleamine 2, 3 dioxygenase 1 (IDO1) in enhancing antitumor immunity in chimeric antigen receptor (CAR)-T cell therapy was investigated. It was found that inhibition of IDO1 activity significantly enhanced CAR-T cell's antitumor effect in esophageal squamous cell carcinoma (ESCC). This suggests that IDO1 inhibitor-loaded nanosheets may provide a promising approach for improving CAR-T cell therapeutic efficacy in solid tumors.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Muhammad Babar Khawar, Haibo Sun
Summary: CAR-engineered T lymphocytes have shown success in treating blood cancers but face limitations such as potential fatal toxicity and high costs. On the other hand, NK cells are the focus of immunological research as promising immunotherapeutic candidates due to their unique recognition mechanisms and powerful cytotoxic effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Jeong Hyeon Cha, Eunsu Kim, Hyeong Ji Lee, Young-Ho Lee, Jeonghyun Lee, Eunha Kim, Chan Hyuk Kim
Summary: Metabolic glycan labeling combined with CAR-T cell therapy can provide novel cancer immunotherapy for solid tumors that lack viable target antigens.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Immunology
Ali Akbar Samadani, Arman Keymoradzdeh, Shima Shams, Armin Soleymanpour, Ali Rashidy-Pour, Houman Hashemian, Sogand Vahidi, Seyedeh Elham Norollahi
Summary: CAR T-cell therapy is a promising immunotherapy for cancer with the potential to treat a variety of solid tumors. By modifying T cells with CAR, it is possible to effectively eliminate certain cancers. Strategies for investigating risks and mitigating off-tumor consequences are crucial, and successful CAR T-cell therapy protocols can enhance the efficacy and safety of treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Oncology
Sara Pagotto, Pasquale Simeone, Davide Brocco, Giulia Catitti, Domenico De Bellis, Simone Vespa, Natalia Di Pietro, Lisa Marinelli, Antonio Di Stefano, Serena Veschi, Laura De Lellis, Fabio Verginelli, Francesco Kaitsas, Manuela Iezzi, Assunta Pandolfi, Rosa Visone, Nicola Tinari, Ignazio Caruana, Mauro Di Ianni, Alessandro Cama, Paola Lanuti, Rosalba Florio
Summary: This review highlights the potential of CAR-T-derived extracellular vesicles (EVs) as therapeutic agents in tumors. CAR-T lymphocytes have shown potential effectiveness in immunotherapy against refractory neoplastic diseases. However, physical barriers, hostile microenvironment, and tumor heterogeneity limit their efficacy against solid tumors. The use of CAR-T-derived EVs as therapeutic agents may enhance CAR-T homing and overcome some adverse effects.
Review
Immunology
Zhengchao Zhang, Lele Miao, Zhijian Ren, Futian Tang, Yumin Li
Summary: CAR-T cells have faced challenges in proliferation and persistence in the treatment of malignancies, leading researchers to genetically modify the cells and introduce immune cytokines to enhance tumor killing function. Releasing immunoregulatory molecules to the tumor region can improve the proliferation ability and persistence of CAR-T cells when in contact with target tumors. Preclinical and clinical trials have shown promising results in enhancing the efficacy of gene-edited interleukin CAR-T cell therapy for malignant tumors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Maoxuan Liu, Junchen Liu, Ziwei Liang, Kun Dai, Jiangyu Gan, Qi Wang, Yang Xu, Youhai H. Chen, Xiaochun Wan
Summary: CAR-M has the potential to improve cancer therapy, and different intracellular signaling domains have different effects on CAR-M functions. CAR-M and CAR-T cells can synergize to kill tumor cells by regulating the expression of costimulatory ligands and polarization of macrophages to enhance cytotoxicity.
Article
Oncology
Jiang Lv, Ruocong Zhao, Di Wu, Diwei Zheng, Zhiping Wu, Jingxuan Shi, Xinru Wei, Qiting Wu, Youguo Long, Simiao Lin, Suna Wang, Zhi Wang, Yang Li, Yantao Chen, Qing He, Suimin Chen, Huihui Yao, Zixia Liu, Zhaoyang Tang, Yao Yao, Duanqing Pei, Pentao Liu, Xuchao Zhang, Zhenfeng Zhang, Shuzhong Cui, Ren Chen, Peng Li
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Shouheng Lin, Xuchao Zhang, Guohua Huang, Lin Cheng, Jiang Lv, Diwei Zheng, Simiao Lin, Suna Wang, Qiting Wu, Youguo Long, Baiheng Li, Wei Wei, Pentao Liu, Duanqing Pei, Yangqiu Li, Zhesheng Wen, Shuzhong Cui, Peng Li, Xiaofang Sun, Yilong Wu, Yao Yao
Summary: This study revealed that intratumoral MDSC infiltration is associated with NSCLC progression and promotes metastasis through producing CCL11, which activates ERK and AKT signaling pathways and induces EMT. High expression of CCL11 is correlated with poor prognosis in lung cancer and other cancer types.
Article
Oncology
Jiang Lv, Le Qin, Ruocong Zhao, Di Wu, Zhiping Wu, Diwei Zheng, Siyu Li, Mintao Luo, Qiting Wu, Youguo Long, Zhaoyang Tang, Yan-Lai Tang, Xuequn Luo, Yao Yao, Li-Hua Yang, Peng Li
Summary: Tumor cells and the immunosuppressive tumor microenvironment inhibit the antitumor activity of T cells through the PD-L1/PD-1 axis. This study reveals that CISH, a member of the SOCS family, plays a critical role in regulating immune checkpoints in T cells. Ablation of CISH in T cells enhances their sensitivity to TCR and cytokine stimulation, and CAR-T cells with CISH deficiency exhibit improved survival, cytokine secretion, and antitumor activity. The study also uncovers a mechanism by which CISH promotes PD-1 expression by suppressing the expression of FBXO38, and proposes a new strategy for enhancing CAR-T cell therapy by inhibiting CISH.
MOLECULAR THERAPY-ONCOLYTICS
(2023)
Article
Medicine, Research & Experimental
Ming Li, Shanglin Li, Ruocong Zhao, Jiang Lv, Diwei Zheng, Le Qin, Siyu Li, Qiting Wu, Youguo Long, Zhaoyang Tang, Yan-Lai Tang, Lihua Yang, Yao Yao, Xuequn Luo, Peng Li
Summary: This study establishes CRC xenografts, evaluates the cytotoxicity and cytokine secretion of anti-CD318 CAR-T cells against CRC, and demonstrates the antitumor activity of CAR318 T cells in vivo.
CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Oncology
Zhiwu Jiang, Rui Liao, Jiang Lv, Shanglin Li, Diwei Zheng, Le Qin, Di Wu, Suimin Chen, Youguo Long, Qiting Wu, Suna Wang, Simiao Lin, Xiaohan Huang, Zhaoyang Tang, Pengcheng Shi, Hongsheng Zhou, Qifa Liu, Ruocong Zhao, Yangqiu Li, Yang Jie, Wei Wei, Peilong Lai, Xin Du, Shuzhong Cui, Robert Weinkove, Pentao Liu, Duanqing Pei, Yao Yao, Peng Li
Summary: CAR-T cell therapies have shown high clinical response rates in B cell malignancies, with potential for solid tumor treatment. Modulating IL-6 trans-signaling through GP130/STAT3 pathway enhances CAR-T cell expansion and antitumor activity, while GP130 expression in CAR-T cells improves antitumor efficacy without systemic IL-6 trans-signaling, indicating the potential for therapeutic applications.
Article
Oncology
Di Wu, Jiang Lv, Ruocong Zhao, Zhiping Wu, Diwei Zheng, Jingxuan Shi, Simiao Lin, Suna Wang, Qiting Wu, Youguo Long, Peng Li, Yao Yao
BIOMARKER RESEARCH
(2020)
Review
Oncology
Jiang Lv, Peng Li
BIOMARKER RESEARCH
(2019)