Article
Biochemistry & Molecular Biology
Dan Xu, Qingfei Li, Yan Zhou, Yanan Shen, Wencong Lai, Tingting Hao, Yi Ding, Kangsen Mai, Qinghui Ai
Summary: This study investigated the functional activities and regulation mechanism of large yellow croaker IFN-gamma in fish macrophages. The results showed that LcIFN-gamma expression was upregulated after LPS and poly(I:C) treatment, and rLcIFN-gamma enhanced phagocytic ability and induced M1 polarization in macrophages. Additionally, rLcIFN-gamma activated the JAK-STAT signaling pathway and upregulated IFN-stimulated gene expression. Furthermore, rLcIFN-gamma also activated other signaling cascades such as MAPK and AKT pathways in addition to the classical JAK-STAT pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Xiang-Yang Zhang, Xinyue Zhuo, Jie Cheng, Xiaohong Wang, Kexin Liang, Xinhua Chen
Summary: This study identified two sub-cloned macrophage cell lines with different morphologies and demonstrated that the transcription factor PU.1 plays a role in regulating the expression of the CTSS gene in these cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Bo Yang, Renlei Ji, Xueshan Li, Wei Fang, Qiuchi Chen, Qiang Chen, Wei Xu, Kangsen Mai, Qinghui Ai
Summary: The study found that autophagy can significantly reduce the gene expression of proinflammatory factors in the liver or hepatocytes, such as tumor necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL1 beta). Inhibition of autophagy can upregulate the gene expression of proinflammatory factors and downregulate the gene expression of anti-inflammatory factors both in vivo and in vitro. Autophagy can alleviate LA-induced inflammatory cytokine gene expression in vivo and in vitro, while inhibition of autophagy leads to the opposite results.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Bin Shen, Siyu Zhang, Fengxin Li, Jing Xu, Yuqin Zhang, Jianshe Zhang
Summary: This study conducted functional analyses for two ISG15 homologues in Larimichthys crocea and found that they play important roles in immune regulation and host immune defense against viral and bacterial infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Manale El Kharbili, Katja Aviszus, Sarah K. Sasse, Xiaoyun Zhao, Karina A. Serban, Susan M. Majka, Anthony N. Gerber, Fabienne Gally
Summary: The resolution of inflammation is an actively regulated process. This study shows that FABP5 and PPARγ reciprocally regulate each other, forming a positive feedback loop that mediates macrophage pro-resolving programming.
Article
Fisheries
Zaiyu Zheng, Hongshu Chi, Xiaodong Liu, Xixi Yang, Xiuxia Chen, Ying Pan, Hui Gong
Summary: A new cell line, YCE1, was established and characterized from Larimichthys crocea for the purpose of in vitro culture models for viral pathogens. YCE1 cells, identified as predominantly fibroblast-like, could form a monolayer at 15-37 degrees C and had an optimum growth temperature of 26 degrees C. The susceptibility test showed noticeable and stable cytopathic effects (CPEs) in the YCE1 cells infected with large yellow croaker iridovirus (LYCIV), making it a viable tool for studying host-pathogen interactions.
Article
Fisheries
Xinyu Zhao, Xiaolong Yin, Tianzhu Ma, Weihua Song, Lihua Jiang, Xiaolin Zhang, Bingjian Liu, Yifan Liu, Xiaojun Yan
Summary: This study investigated the effects of different concentrations of chloroquine (CQ) on large yellow croakers and found that higher concentrations of CQ led to more obvious toxic effects, resulting in high mortality rates. The study also observed differential expressions of autophagy/inflammation/apoptosis-related genes in representative tissues.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Jie Ding, Yibo Zhang, Xujie Li, Jiaying Wang, Xinming Gao, Qiumeng Xiang, Zhenyu Gao, Tianyi Lan, Songpeng Jia, Mengqi Lu, Ran Meng, Xuelei Wang, Xiongfei Wu, Junquan Zhu, Weiliang Shen
Summary: This study aimed to improve the hypoxia tolerance of farmed Larimichthys crocea using genomic selection. By genotyping a large number of samples using a SNP array, it was found that GBLUP was the best genomic selection model. The results confirmed that genomic selection can effectively enhance the hypoxia tolerance in L. crocea.
Review
Immunology
Daniel Toobian, Pradipta Ghosh, Gajanan D. Katkar
Summary: This review article focuses on the potential roles of peroxisome proliferator-activated receptors (PPARs) in macrophage biology, as well as the modulation of immune cell function, microbial infection, and inflammatory diseases by dual and pan PPAR agonists.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Dan Zhao, Chao Xue, Yanfang Yang, Jiaqi Li, Xiaolin Wang, Yi Chen, Shuang Zhang, Yuanli Chen, Yajun Duan, Xiaoxiao Yang, Jihong Han
Summary: This study demonstrates the important roles of PPARγ and Nogo-B in liver fibrosis. PPARγ deficiency increases susceptibility of mice to CCl4-induced liver injury and fibrosis, while Nogo-B deficiency effectively attenuates this exacerbation effect. Therefore, Nogo-B inhibition may be a potential therapeutic approach for liver fibrosis treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Fisheries
Xiang-Yu Wu, Li Nie, Xin-Jiang Lu, Chen-Jie Fei, Jiong Chen
Summary: In this study, the role of PPAR gamma in immune regulation in teleost fish was investigated. Results showed that the expression of LcPPAR gamma changed during infection and DHA treatment, and was associated with the expression of immune-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Qiangde Liu, Si Zhu, Zengqi Zhao, Tingting Hao, Xiang Xu, Shangzhe Han, Yueru Li, Kangsen Mai, Qinghui Ai
Summary: LcTFEB plays a critical role in the regulation of antiviral immune responses in fish macrophages. It acts as a negative feedback mediator, limiting virus-induced inflammatory responses. Its activity is independent of mTORC1 and is positively correlated with the expression of antiviral immune genes.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yongyang Wang, Ziliang Wu, Hui Chen, Ruoyu Liu, Weini Zhang, Xinhua Chen
Summary: Astragalus polysaccharides (APS) can protect large yellow croaker macrophages against inflammatory injury by activating NF-kappa B and pyroptosis signaling pathways. This research enhances our understanding of the immune regulation mechanism of APS in fish and facilitates its application in the prevention and control of fish bacteriosis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jie Sun, Kangsen Mai, Qinghui Ai
Summary: Endoplasmic reticulum (ER) homeostasis is important for cell physiological functions and disruption of ER homeostasis can lead to ER stress and inflammation. Glucose-regulated protein 78 (GRP78), an ER chaperone, is crucial for maintaining cellular homeostasis, but its effects on ER stress and inflammation in fish are not fully understood. In this study, ER stress and inflammation were induced in macrophages of large yellow croakers by tunicamycin (TM) or palmitic acid (PA), and the effects of GRP78 agonist/inhibitor were examined. The results showed that the TM/PA treatment induced ER stress and inflammation, which were reduced by GRP78 agonist treatment, while GRP78 inhibitor treatment further induced ER stress and inflammation. These findings provide novel insights into the relationship between GRP78 and TM/PA-induced ER stress or inflammation in large yellow croakers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Xiaojie Wang, Yaoyi Feng, Zichao Zhang, Chenchen Li, Huan Han
Summary: Large yellow croaker, a commercially important fish species, is sensitive to sound. The impact of ocean acidification on its auditory system was investigated by exposing the fish to different CO2 concentrations. It was found that ocean acidification can cause irregular shape and rough surface of the inner ear otoliths, and impair the fish's ability to maintain balance, potentially posing a threat to individuals and populations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
