期刊
FRONTIERS IN MICROBIOLOGY
卷 12, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.655845
关键词
Vibrio vulnificus; DNAzyme; rapid detection; fluorescence sensor; aquatic products
类别
资金
- National Key R&D Program of China [2018YFC0311106]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [ZD201918]
- Postgraduate Research and Practice Innovation Program of Jiangsu [KYCX19-2296, KYCX20-2887]
A DNAzyme-based fluorescent sensor was developed for the detection of V. vulnificus in aquatic products, with a detection limit of 2.2 x 10(3) CFU/ml and results obtainable within 5-10 minutes. The DNAzyme exhibited high activity, specificity, and sensitivity.
Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated RFD-VV-M2 exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 x 10(3) CFU/ml, and results could be obtained within 5-10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.
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