Article
Immunology
Walker N. Oliveira, Andreza S. Dorea, Pedro P. Carneiro, Mauricio T. Nascimento, Lucas P. Carvalho, Paulo R. L. Machado, Albert Schriefer, Olivia Bacellar, Edgar M. Carvalho
Summary: The results suggest that DL isolates infected more monocytes, induced greater respiratory burst, and more cytokine production compared to isolates from CL patients. However, greater parasite multiplication and higher TLR2 and TLR4 expression were seen in monocytes from DL patients compared to CL following infection with DL isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Taina Lago, Lucas P. Carvalho, Mauricio Nascimento, Luiz H. Guimaraes, Jamile Lago, Lea Castellucci, Augusto M. Carvalho, Alex Lago, Edgar M. Carvalho
Summary: Obesity modifies the clinical presentation of cutaneous leishmaniasis caused by Leishmania braziliensis and is associated with greater failure in therapy, as obese patients may present hypertrophic ulcers rather than typical oval, ulcerated lesions and have lower cure rates compared to overweight and normal weight individuals.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Andrea Magalhaes, Lucas P. Carvalho, Rubia Costa, Monica S. Pita, Thiago Marconi Cardoso, Paulo R. L. Machado, Edgar M. Carvalho, Sergio Arruda, Augusto M. Carvalho
Summary: This study showed that high levels of anti-Leishmania IgG and IgG2 are characteristic of disseminated leishmaniasis (DL), with IgG correlated with pro-inflammatory cytokines and IgG2 directly correlated with the number of lesions in DL.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Rabiaa M. Sghaier, Fouad Benhnini, Fatma Z. Guerfali, Hanene Attia, Aymen Bali, Amor Zaatour, Ghada Mkannez, Adel Gharbi, Nabil Belhaj-Hamida, Hichem Dridi, Afif Ben-Salah, Koussay Dellagi, Dhafer Laouini
Summary: The scars of healed HCL caused by L. major do not contain detectable residual parasites, suggesting a sterile cure. This is in contrast to other Leishmania species that cause chronic or recurrent forms of leishmaniasis, where parasites do persist in healed lesions. This finding highlights the importance of considering alternative mechanisms for long-term immunity and vaccine development against L. major infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Beatriz Coutinho de Oliveira, Ailton Alvaro da Silva, Marton Kaique de Andrade Cavalcante, Maria Edileuza Felinto de Brito, Maria Carolina Accioly Brelaz de Castro, Vanessa Lucilia Silveira de Medeiros, Rafael de Freitas e Silva, Valeria Rego Alves Pereira
Summary: Cutaneous Leishmaniasis (CL) is a skin ulcer disease caused by protozoans, and there is no vaccine available. Previous research identified T cell epitopes of Leishmania braziliensis and tested their ability to rescue memory T cells during natural infection. The study found increased frequencies of TCM and TEM, especially in the CD8 compartment, during CL. These results suggest a potentially immunogenic profile of the peptides and support the development of anti-Leishmania formulations.
Article
Immunology
Paulo R. L. Machado, Fernanda V. O. Prates, Viviane Boaventura, Taina Lago, Luiz H. Guimaraes, Albert Schriefer, Temis W. F. Corte, Gerson Penna, Aldina Barral, Manoel Barral-Netto, Edgar M. Carvalho
Summary: The study showed that miltefosine is more effective than pentavalent antimony for treating cutaneous leishmaniasis caused by L. braziliensis in Brazil, and it accelerates healing time. However, combining miltefosine with GM-CSF did not improve therapeutic outcome but did shorten the healing time.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Microbiology
Augusto M. Carvalho, Olivia Bacellar, Edgar M. Carvalho
Summary: The study explores the pathogenesis of American tegumentary leishmaniasis (ATL) and suggests that individuals with subclinical L. braziliensis infection exhibit lower respiratory burst and IFN-γ production, but more efficient killing of the parasite. Increased inflammation and cytotoxic gene expression in CL tissue samples contribute to additional damage to skin and mucosal tissues.
Article
Public, Environmental & Occupational Health
Michal Solomon, Ariel Gimple, Inbal Fuchs, Assi Cicurel, Tal Meninger, Dror Avni, Abed Nasereddin, Charles L. Jaffe, Eli Schwartz
Summary: The study aimed to investigate the prevalence of Leishmania in the blood of patients with active or previous cutaneous leishmaniasis (CL). The findings of this pilot study suggest that people with active or recent CL caused by L. major and L. tropica do not harbor parasites in their blood. Hence, their exclusion from blood donation should be revisited.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Immunology
Augusto M. Carvalho, Luiz H. Guimaraes, Rubia Costa, Maira G. Saldanha, Iana Prates, Lucas P. Carvalho, Sergio Arruda, Edgar M. Carvalho
Summary: In patients with negative LST, there is a lower cure rate and impaired Th1 response, as well as an increased frequency of CD8(+) T cells and reduced inflammation in lesions. Restoration of Th1 response is associated with cure of the disease. Increased production of inflammatory cytokines, granzyme B, and MMP-9 contributes to immunopathology in LST-negative patients.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Clara Monica F. de Lima, Andrea S. Magalhaes, Rubia Costa, Carolina C. Barreto, Paulo R. L. Machado, Edgar M. Carvalho, Marcus M. Lessa, Lucas P. Carvalho
Summary: Mucosal leishmaniasis patients in advanced stages had higher levels of IgG and IgG1 antibodies, with reductions in IgG and IgG2 antibodies post-treatment. This suggests a link between IgG antibody levels and disease severity, with potential for assessing therapeutic response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Michal Solomon, Nadav Astman, Karin Warshavsky, Aviv Barzilai, Tal Meningher, Dror Avni, Eli Schwartz
Summary: This article reports 8 cases of Leishmania infantum cutaneous leishmaniasis in Israel, with 7 cases occurring during 2020-2021, suggesting that L. infantum may be an emerging infection in Israel.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Pharmacology & Pharmacy
Rafaela Miranda Barbosa, Malu Mateus Santos Obata, Jose Rodrigues do Carmo Neto, Rhanoica Oliveira Guerra, Anna Victoria Bernardes e Borges, Rafael Obata Trevisan, Leticia Cirelli Ruiz, Julia de Moura Bernardi, Ana Carolina de Morais Oliveira-Scussel, Sarah Cristina Sato Vaz Tanaka, Fernanda Bernadelli de Vito, Fernanda Rodrigues Helmo, Thais Soares Farnesi de Assuncao, Juliana Reis Machado, Carlo Jose Freire de Oliveira, Virmondes Rodrigues Junior, Anielle Christine Almeida Silva, Marcos Vinicius da Silva
Summary: The study demonstrates that Ag-ZnO/xAgO nanocomposites can inhibit the replication of L.b with high selectivity. The nanocomposites also modulate the activation and cytokine production of human peripheral blood mononuclear cells towards a proinflammatory phenotype.
Article
Parasitology
Marina Floro e Silva, Guilherme Augusto Sanches Roque, Diullia de Andrade Machado, Francisca Janaina Soares Rocha, Selma Giorgio
Summary: Leishmaniasis is a neglected disease caused by the protozoan Leishmania. In this study, the course of infection in mice inoculated with two reference strains of L. (V.) braziliensis was investigated. The results showed that both strains induced footpad lesions, but the lesions in BA infected mice healed while the lesions in CE infected mice did not regress. The presence of parasites was detected in various tissues of both groups of infected mice. This study provides new insights into the pathogenesis and progression of L. (V.) braziliensis infection in mice.
Article
Immunology
Mauricio T. Nascimento, Debora L. Viana, Fabio C. Peixoto, Sergio M. Arruda, Edgar M. Carvalho, Lucas P. Carvalho
Summary: The increased levels of prostaglandin E2 (PGE2) can weaken the microbicidal function of macrophages against Leishmania braziliensis, leading to disease severity and therapeutic failure in cutaneous leishmaniasis (CL) patients. Inhibiting the activity of COX-2 with the selective NSAID NS398 can enhance macrophage killing of L. braziliensis and protect against pathological inflammatory response.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Natalia Munoz-Durango, Alexander Gomez, Natalia Garcia-Valencia, Miguel Roldan, Marcela Ochoa, David E. Bautista-Erazo, Jose R. Ramirez-Pineda
Summary: This study successfully developed a mouse model of L(V)p cutaneous leishmaniasis that replicates the characteristics of the human disease. The model induces a progressive cutaneous disease with ulcerated lesions and indurated borders, similar to human CL, by infecting BALB/c mice intradermally with L(V)p promastigotes. The model also exhibits regional lymphadenopathy and lymphatic dissemination of parasites to draining lymph nodes. A mixed adaptive T cell-mediated response and a humoral adaptive response are induced in the model, with a redirection from a non-protective Th2 response to a protective Th1 response. This novel mouse model is valuable for studying the pathogenesis, immunity, and therapeutics of L(V)p infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Eduardo Milton Ramos-Sanchez, Luiza Campos Reis, Marina de Assis Souza, Sandra Marcia Muxel, Kamila Reis Santos, Dimitris Lagos, Valeria Rego Alves Pereira, Maria Edileuza Felinto de Brito, Paul Martin Kaye, Lucile Maria Floeter-Winter, Hiro Goto
Summary: This study investigated the changes in miRNA associated with leishmaniasis and identified different target pathways in immune and inflammatory pathways. miR-548d-3p may play a role in suppressing parasite growth, but further research is needed to understand its in vivo effects and its potential as a biomarker for progressive leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Correction
Immunology
Eduardo Milton Ramos-Sanchez, Luiza Campos Reis, Marina de Assis Souza, Sandra Marcia Muxel, Kamila Reis Santos, Dimitris Lagos, Valeria Rego Alves Pereira, Maria Edileuza Felinto de Brito, Paul Martin Kaye, Lucile Maria Floeter-Winter, Hiro Goto
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Carolina Menezes Suassuna de Souza, Ewerton de Souza Lima, Raphael Ferreira Ordonho, Bianca Rafaella Rodrigues dos Santos Oliveira, Rebeca Cordeiro Rodrigues, Marquiliano Farias de Moura, Daniel Magalhaes Lima, Maiara Garcia Blagitz, Eduardo Milton Ramos Sanchez, Isac Almeida de Medeiros, Fernando Nogueira Souza, Artur Cezar de Carvalho Fernandes
Summary: This study evaluated the expression of immune checkpoints PD-1 and CTLA-4 in blood T lymphocytes during the periparturient period in dairy cows. The expression of these immune checkpoints was found to be associated with uterine health and immune tolerance. Additionally, the serum acute phase-protein haptoglobin concentration was related to the expression of these immune checkpoints. However, there was no association between the indicators of negative energy balance and the expression of the immune checkpoint markers.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Felipe R. Floreste, Braz Titon Jr, Stefanny C. M. Titon, Sandra M. Muxel, Aymam C. de Figueiredo, Fernando R. Gomes, Vania R. Assis
Summary: This study highlights the crucial role of the liver in the inflammatory response in amphibians. The expression of immune proteins in the liver was up-regulated in the early phase, while the spleen exhibited a slower but more consistent response.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Infectious Diseases
Kamila R. Santos, Fernando N. Souza, Eduardo M. Ramos-Sanchez, Camila F. Batista, Luiza C. Reis, Wesley L. Fotoran, Marcos B. Heinemann, Adriano F. Cunha, Mussya C. Rocha, Angelica R. Faria, Helida M. Andrade, Monica M. O. P. Cerqueira, Magnus Gidlund, Hiro Goto, Alice Maria M. P. Della Libera
Summary: This study investigated the immunological response elicited by the recombinant S. aureus-cure-associated proteins in combination with the GM-CSF DNA vaccination. The results showed that the vaccination induced a type 3 immunity, which is important for developing an effective bovine S. aureus mastitis vaccine.
Article
Agriculture, Dairy & Animal Science
Fernando N. Souza, Kamila R. Santos, Jose A. Ferronatto, Eduardo M. Ramos Sanchez, Bruno Toledo-Silva, Marcos B. Heinemann, Sarne De Vliegher, Alice M. M. P. Della Libera
Summary: This study investigated whether different staphylococcal and mammaliicoccal species and strains can induce B and T lymphocyte proliferation and production of interleukin-17A (IL-17A) and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMCs) of nulliparous, primiparous, and multiparous dairy cows. Flow cytometry was used to measure lymphocyte proliferation, specific antibodies were used to identify different lymphocyte populations, and the PBMC culture supernatant was used to measure IL-17A and IFN-gamma production. The results showed that certain strains of Staphylococcus aureus and Staphylococcus chromogenes triggered T lymphocyte proliferation and increased IL-17A and IFN-gamma production. Conversely, Mammaliicoccus fleurettii and other strains had no effect on lymphocyte proliferation. Multiparous cows had higher B lymphocyte proliferation and cytokine production compared to primiparous and nulliparous cows. Phytohemagglutinin M-form specifically stimulated T cell proliferation.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Jonathan Miguel Zanatta, Stephanie Maia Acuna, Yan de Souza Angelo, Camilla de Almeida Bento, Jean Pierre Schatzman Peron, Beatriz Simonsen Stolf, Sandra Marcia Muxel
Summary: This study investigated the effects of L-arginine and polyamines on L. amazonensis infection and the transcriptional regulation of genes related to arginine metabolism and proinflammatory response in BALB/c macrophages. The results showed that putrescine supplementation reduced the percentage of infected macrophages compared to other supplements. Additionally, the availability of polyamines was found to interfere with cytokine/chemokine production.
Article
Multidisciplinary Sciences
Mahyumi Fujimori, Ruth Tamara Valencia-Portillo, Jose Angelo Lauletta Lindoso, Beatriz Julieta Celeste, Roque Pacheco de Almeida, Carlos Henrique Nery Costa, Alda Maria da Cruz, Angelita Fernandes Druzian, Malcolm Scott Duthie, Carlos Magno Castelo Branco Fortaleza, Ana Lucia Lyrio de Oliveira, Anamaria Mello C. Miranda Paniago, Igor Thiago Queiroz, Steve Reed, Aarthy Vallur, Hiro Goto, Maria Carmen Arroyo Sanchez
Summary: The performance of recombinant antigens K18 and KR95 in serological diagnosis was evaluated and compared with rK28 and rK39. The results showed that rKR95 had higher sensitivity and specificity in the diagnosis of visceral leishmaniasis, suggesting its use in serological assays.
Article
Immunology
Alice Maria Melo do Nascimento, Carolina Menezes Suassuna de Souza, Ana Claudia Dumont Oliveira, Maiara Garcia Blagitz, Eduardo Milton Ramos Sanchez, Alice Maria Melville Paiva Della Libera, Ricardo de Miranda Henriques Leite, Artur Cezar de Carvalho Fernandes, Fernando Nogueira Souza
Summary: This study explored the impact of BLV infection in the periparturient period on the expression of PD-1 and CTLA-4 in blood T cells, as well as the rate of new intramammary infections during early lactation. The results showed that BLV-infected dairy cows had higher expression of CTLA-4 and PD-1 in blood T cells, and a higher rate of new intramammary infections during early lactation.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Microbiology
Guilherme S. de Moura, Eneas de Carvalho, Eduardo M. Ramos Sanchez, Fabio P. Sellera, Michele F. S. Marques, Marcos B. Heinemann, Sarne De Vliegher, Fernando N. Souza, Rinaldo A. Mota
Summary: The discovery of mecA and mecC-positive mammaliicocci from American continent represents a new reservoir of antimicrobial resistance genes, indicating a possible emergence of M. sciuri as a globally important pathogen. These M. sciuri strains carried a wide range of antimicrobial resistances, virulence-associated genes, and belonged to a globally disseminated branch associated with farm animals, companion animals, and food. The findings highlight the need to monitor M. sciuri under the One Health approach due to its potential spread at the human-animal-environment interface.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Carolina Menezes Suassuna de Souza, Maiara Garcia Blagitz, Fernando Nogueira Souza, Camila Freitas Batista, Daniel Magalhaes Lima, Alexandre Jose Alves, Marcos Bryan Heinemann, Eduardo Milton Ramos Sanchez, Hugo Frias Torres, Artur Cezar de Carvalho Fernandes, Alice Maria Melville Paiva Della Libera
Summary: This study investigated the predictive values of milk leukocyte differentials (MLD) for improving the diagnosis of intramammary infections (IMIs) and subclinical mastitis. The results showed that the combination of T CD8+ lymphocytes with PMNLs and macrophages can better diagnose bovine IMIs.
Article
Biochemistry & Molecular Biology
Stephanie Maia Acuna, Jonathan Miguel Zanatta, Camilla de Almeida Bento, Lucile Maria Floeter-Winter, Sandra Marcia Muxel
Summary: This study identified miR-294 and miR-410 as biomarkers for regulating the inflammatory profiles of macrophages during L. amazonensis infection. The inhibition of miR-294 and miR-410 showed potential therapeutic strategies for modulating inflammation and reducing parasitism.