Article
Biotechnology & Applied Microbiology
Ekta Khare, Naveen Kumar Arora
Summary: Biosurfactants are environment-friendly molecules with multifunctional properties that can be useful in various industries. The novel biosurfactant-producing isolate Pseudomonas guariconensis LE3 showed potential as a biostimulant and biocontrol agent against charcoal rot disease in sunflowers. The biosurfactant-amended formulation of LE3 cells demonstrated the highest yield enhancement and biocontrol activity, improving plant-bacterial interaction and soil properties for better disease control and plant health.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Infectious Diseases
Ashwani Kumar, Hemanth Kumar, A. M. Manonmani, G. Prabakaran, B. Vijayakumar, A. Mathivanan, I Geetha, P. Jambulingam
Summary: The efficacy, application dosage, residual activity, and frequency of application of two mosquitocidal biosurfactants, surfactin (VCRC B471) and di-rhamnolipid (VCRC B426), in controlling Anopheles stephensi immatures in selected sites in Goa, India, were evaluated. The results showed that both biosurfactants were effective in reducing the density of immature mosquitoes for a duration of 14-18 days, with optimum application dosages of 51 mL/m(2) and 27 mL/m(2) for surfactin and di-rhamnolipid, respectively.
JOURNAL OF VECTOR BORNE DISEASES
(2022)
Article
Pharmacology & Pharmacy
Matthew S. S. Twigg, Simms A. A. Adu, Suguru Sugiyama, Roger Marchant, Ibrahim M. M. Banat
Summary: Glycolipid-type biosurfactants have shown potential as natural anticancer agents. In this study, mono-rhamnolipids produced by a recombinant strain of Pseudomonas aeruginosa were used to treat colorectal cancer cells. The mono-rhamnolipids significantly reduced the viability of the cancer cells and showed higher cytotoxicity compared to Piceatannol, another natural anticancer agent. The compounds appeared to induce necrosis in the cancer cells.
Article
Engineering, Chemical
Mohd Nazren Radzuan, James Winterburn, Ibrahim Banat
Summary: The study demonstrates the successful production of rhamnolipid biosurfactant using PFAD and FAME by P. aeruginosa PAO1. The produced rhamnolipids were predominantly monorhamnolipid and dirhamnolipid, showing potential for high added value in various applications.
Letter
Biochemical Research Methods
Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, a novel transcription-associated fluorescence-activated droplet sorting method was developed for specific targeting of di-RL hyperproducers. Using this method, ten higher di-RL producing microorganisms were screened out, which showed significantly increased di-RL yield compared to the model strain. This study provides a rapid, reliable screening method for di-RL production and offers a way to measure di-RL yield in populations of droplets containing different environmental colonies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Physical
Alessandra Marega Motta, Maressa Donato, Giovanna Mobbili, Paolo Mariani, Rosangela Itri, Francesco Spinozzi
Summary: This study investigates the mechanisms of interaction between Rhamnolipids (RLs) and simulated plasma membrane models. The results show that mono-RL primarily interacts with the outer leaflet of the membrane through insertion without pore formation, but induces lipid mixing. On the other hand, di-RLs initially interact through insertion and later undergo pore formation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jing Zhou, Si-Jia Miao, Shi-Zhong Yang, Jin-Feng Liu, Hong-Ze Gang, Bo-Zhong Mu
Summary: A new sensitive method for the quantitative analysis of rhamnolipid was developed based on a simple derivatization reaction. The established method showed good reproducibility and accuracy, and was successfully applied to the quantitative analysis of rhamnolipid homologs.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Madhumitha Narasimman, Vijayakumar Natesan, Vijay Mayakrishnan, Jayaprakash Rajendran, Amalan Venkatesan, Sung-Jin Kim
Summary: The nanoemulsion of Mentha pipertia leaves showed good larvicidal activity against Anopheles mosquitoes, making it a promising low-cost and environmentally friendly insecticide.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Energy & Fuels
Yan Feng, Jianlong Xiu, Lina Yi, Bo Wu, Lixin Huang, Yuandong Ma, Li Yu
Summary: In this study, a genetically engineered strain of Pseudomonas aeruginosa named WJPAB was constructed by replacing the original promoter of the rhlAB gene with a strong promoter. The optimal carbon source for WJPAB was rapeseed oil, and under the optimal fermentation conditions, the product yield of WJPAB was significantly higher than that of the wild strain. The metabolites of WJPAB were confirmed to be rhamnose homologues and exhibited excellent surface activity and stability under various environmental conditions. The compound system of fermentation broth and xanthan gum showed promising results in enhancing oil recovery.
Article
Energy & Fuels
Paria Safari, Morteza Hosseini, Mostafa Lashkarbolooki, Monireh Ghorbani, Ghasem Najafpour Darzi
Summary: In this study, rice bran oil extracted from agricultural waste was used as a renewable carbon source for biosurfactant production. The biosurfactant showed good performance in reducing surface tension and exhibited potential antifungal properties. It has the potential to be a bio-adjuvant and a replacement for chemical surfactants in addressing oil spills and environmental decontamination processes.
JOURNAL OF PETROLEUM EXPLORATION AND PRODUCTION TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Veronica Segovia, Antonio Reyes, Gildardo Rivera, Pedro Vazquez, Gonzalo Velazquez, Alma Paz-Gonzalez, Regina Hernandez-Gama
Summary: This study focused on producing and characterizing biosurfactants using Thermoanaerobacter sp. CM-CNRG TB177 strain, and evaluating the impact of different carbon and nitrogen sources on the surfactant's ability to reduce water surface tension. The results showed that the strain produces a rhamnolipid mixture, with molasses as a carbon source significantly affecting the surfactant's efficiency. Additionally, it was found that the microorganism requires yeast extract as a nitrogen source for growth, and this study provides the first description of biosurfactant produced by bacteria of the Thermoanaerobacter genus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Brian Gidudu, Evans M. Nkhalambayausi Chirwa
Summary: This study discovered that Pseudomonas aeruginosa strain could produce biosurfactants in an electrochemical environment, but the survival and yield were affected by the intensity of the electric field applied, with the highest current leading to bacterial inactivation and elimination.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Microbiology
Muthusamy Sanjivkumar, Murugan Deivakumari, Grasian Immanuel
Summary: Bio-surfactants, specifically rhamnolipid produced by Pseudomonas aeruginosa (DKB1), exhibit potent biomedical properties including antibacterial, antioxidant, and anticancer activities. The rhamnolipid showed effectiveness against various bacteria strains, significant antioxidant activities, and potential anticancer effects on different cancer cell lines. The study concludes that rhamnolipid derived from oil-polluted areas has promising biomedical applications.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Feng Zhao, Bingxin Wang, Menglin Yuan, Sijia Ren
Summary: This study discovered that mono-rhamnolipid was superior to di-rhamnolipid in terms of antimicrobial activity for agricultural applications. Mono-rhamnolipid is an excellent candidate for agricultural biocontrol, and the knockout strain P. aeruginosa SG Delta rhlC is an excellent microbial cell factory for high production of mono-rhamnolipid.
MICROBIAL CELL FACTORIES
(2022)
Article
Environmental Sciences
Arulazhagan Pugazhendi, Mamdoh T. Jamal, Bandar A. Al-Mur, Rajesh Banu Jeyakumar, Gopalakrishnan Kumar
Summary: This study successfully increased the liquefaction efficiency and hydrogen production of Ulva reticulata biomass by the combinative pretreatment of mechanical and biosurfactant.
Article
Parasitology
Kannadasan Shankar, Gnanasundaram Prabakaran, Arulsamy M. Manonmani
Article
Anthropology
Rati Devendra, Vinod Gupta, Somashekhar S. Biradar, Pradeep Bhat, Shantharam Hegde, S. L. Hoti, Malay B. Mukherjee, Harsha Hegde
ANNALS OF HUMAN BIOLOGY
(2020)
Article
Infectious Diseases
Bethan V. Purse, Narayanaswamy Darshan, Gudadappa S. Kasabi, France Gerard, Abhishek Samrat, Charles George, Abi T. Vanak, Meera Oommen, Mujeeb Rahman, Sarah J. Burthe, Juliette C. Young, Prashanth N. Srinivas, Stefanie M. Schafer, Peter A. Henrys, Vijay K. Sandhya, M. Mudassar Chanda, Manoj V. Murhekar, Subhash L. Hoti, Shivani K. Kiran
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Medicine, Research & Experimental
Laxminarayan Rawat, Harsha Hegde, Sugeerappa Laxmanappa Hoti, Vijayashree Nayak
BIOMEDICINE & PHARMACOTHERAPY
(2020)
Article
Infectious Diseases
Rati Devendra, Vinodkumar Gupta, Rajasubramaniam Shanmugam, M. P. S. S. Singh, Purushottam Patel, Neena Valecha, Neelima Mishra, Naseem Ahmed, S. L. Hoti, Harsha V. Hegde, Prashant Warang, Ashish Chiddarwar, Prabhakar Kedar, Pramod Mayekar, Malay B. Mukherjee
INFECTION GENETICS AND EVOLUTION
(2020)
Review
Infectious Diseases
Sarah J. Burthe, Stefanie M. Schafer, Festus A. Asaaga, Natrajan Balakrishnan, Mohammed Mudasssar Chanda, Narayanaswamy Darshan, Subhash L. Hoti, Shivani K. Kiran, Tanya Seshadri, Prashanth N. Srinivas, Abi T. Vanak, Bethan V. Purse
Summary: Zoonotic diseases, particularly affecting tropical communities, are often managed through human medical treatments, but there are alternative interventions targeting vectors or wildlife hosts that may be more effective. Improved ecological understanding can lead to a broader suite of management options.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Public, Environmental & Occupational Health
F. A. Asaaga, J. C. Young, M. A. Oommen, R. Chandarana, J. August, J. Joshi, M. M. Chanda, A. T. Vanak, P. N. Srinivas, S. L. Hoti, T. Seshadri, B. Purse
Summary: This study explores the facilitators of and barriers to successful convergence between the human, animal, and environmental health sectors in India to address zoonotic diseases. Despite the importance of cross-sectoral collaboration, factors like limited policy visibility, conflicting departmental priorities, and institutional capacities hinder effective collaboration. Strengthening national policy frameworks and building on existing informal relationships and collaboration platforms are suggested as ways forward for improved disease surveillance and interventions.
Article
Infectious Diseases
N. Pradeep Kumar, Ashwani Kumar, D. Panneer, S. Abidha, S. Muthukumaravel, T. Sankari, P. M. Ajithlal, Jessu Mathew, Suhana Koothradan, R. Paramasivan, M. Muniyaraj, Himmat Singh, Rekha Saxena, P. Vijayachari, I. P. Sunish, A. N. Shriram, Prafulla Dutta, Saurav Jyoti Patgiri, D. R. Bhattacharyya, S. L. Hoti, D. Chattopadhyay, Subarna Roy, Namita Mahapatra, Sanghamitra Pati, Gyan Chand, A. K. Mishra, Pradip Barde, P. Jambulingam
Summary: The Indian Council of Medical Research conducted nationwide vector surveillance for Zika and Dengue viruses in India from 2016 to 2019. The results showed no active transmission of the American lineage-pandemic Zika virus in India during the study period.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Kalichamy Alagarasu, Poonam Patil, Meenakshi Kaushik, Deepika Chowdhury, Rajesh K. Joshi, Harsha V. Hegde, Mahadeo B. Kakade, Sugeerappa Laxmanappa Hoti, Sarah Cherian, Deepti Parashar
Summary: This study conducted in vitro antiviral screening of plant extracts and purified compounds for their activities against dengue and chikungunya viruses. The extracts from Plumeria alba, Ancistrocladus heyneanus, Bacopa monnieri, and Cucurbita maxima showed activity against both viruses, while the extract from Vitex negundo only showed activity against dengue virus. Among the purified compounds, anacardic acid, chloroquinone, and methyl gallate showed activity against dengue virus, and only methyl gallate had activity against chikungunya virus. These findings provide potential candidates for the development of phytopharmaceutical drugs against dengue and chikungunya.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
S. Sabesan, K. Krishnamoorthy, S. L. Hoti, S. Subramanian, A. Srividya, Nupur Roy, Tanu Jain, Ashwani Kumar, Manju Rahi
Summary: Lymphatic filariasis (LF) is a vector-borne neglected tropical disease that India aims to eliminate through mass drug administration (MDA) programs, but the progress has been suboptimal. To address poor compliance, the use of DEC-fortified salt is proposed as a well-researched approach recommended by the World Health Organization. This strategy has been successful in small- and large-scale trials and offers a safe, less expensive, more efficient, and prompt solution for LF elimination.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Infectious Diseases
Ashwani Kumar, Hemanth Kumar, A. M. Manonmani, G. Prabakaran, B. Vijayakumar, A. Mathivanan, I Geetha, P. Jambulingam
Summary: The efficacy, application dosage, residual activity, and frequency of application of two mosquitocidal biosurfactants, surfactin (VCRC B471) and di-rhamnolipid (VCRC B426), in controlling Anopheles stephensi immatures in selected sites in Goa, India, were evaluated. The results showed that both biosurfactants were effective in reducing the density of immature mosquitoes for a duration of 14-18 days, with optimum application dosages of 51 mL/m(2) and 27 mL/m(2) for surfactin and di-rhamnolipid, respectively.
JOURNAL OF VECTOR BORNE DISEASES
(2022)
Article
Immunology
Venkatesan Vasuki, Sugeerappa Hoti, Swaminathan Subramanian, Abdul Khan, Velayudham Thenmozhi, Nagarajan Ananganallur, Namita Mahapatra, Ramalingam Balasubramaniyan
Summary: The study developed an infective stage specific RT-PCR assay for Wuchereria bancrofti and evaluated its sensitivity and specificity in detecting infective larvae. The results showed that the assay was highly sensitive and specific, comparable to conventional mosquito dissection techniques, suggesting its potential as a xenomonitoring tool in LF elimination programmes.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2021)
Article
Parasitology
Thirumal Sankari, Swaminathan Subramanian, Subhash L. Hoti, Subhada P. Pani, Purushothaman Jambulingam, Pradeep K. Das
Summary: The study found individual variations in response to DEC, with 65.2% of individuals showing no response in the 12-month post-treatment period. Treatment response was independent of age, gender, and time, with overall response increasing in low microfilariae density categories and decreasing in high density categories. Random coefficient estimates showed greater variability in response between individuals over time post-treatment. These results emphasize the importance of studying host genetic factors associated with DEC action.
PARASITOLOGY RESEARCH
(2021)
Review
Public, Environmental & Occupational Health
Joleen Savianne Almeida, Ajeet Kumar Mohanty, Savita Kerkar, Sugeerappa Laxmanappa Hoti, Ashwani Kumar
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE
(2020)
Article
Immunology
Savitha Chellappan, Subarna Roy, Jyoti M. Nagmoti, Wahida Tabassum, S. L. Hoti, Mrinal Kanti Bhattacharyya, Praveen Balabaskaran Nina
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Article
Parasitology
Gabriela Martinez Moura Tavares, Hannar Angelica de Melo Alverga, Estela Moraes Felix, Giovana de Oliveira Dichman, Gabriel Monteiro Pinheiro, Yara Juliano, Priscila Paruci, Dennis Minoru Fujita, Lucas Melo Neves, Marina Tiemi Shio, Luiz Henrique da Silva Nali
Summary: This study investigated the frequency of N. gonorrhoeae infection in women in a poor area of São Paulo and found that most of the strains detected showed resistance to one or more antimicrobials.
Article
Parasitology
Qian Yao, Ying-Ying Fan, Shuang Huang, Gui-Rong Hu, Jun-Ke Song, Xin Yang, Guang-Hui Zhao
Summary: This study found that the expression of miR-4521 was upregulated during C. parvum infection, and this upregulation was related to the TLR/NF-kappa B signal pathway. Additionally, miR-4521 promoted the propagation of C. parvum in HCT-8 cells by regulating BCL2-mediated cell apoptosis through targeting foxm1.
Article
Parasitology
Cho Naing, Maxine A. Whittaker, Wong Siew Tung, Htar Aung, Joon Wah Mak
Summary: This study aimed to estimate the prevalence of zoonotic brugian filariasis in animals and compare it with the prevalence in humans in the same area. The findings suggest that cats, dogs, and leaf-eating monkeys may serve as potential reservoirs for human brugian filariasis.
Review
Parasitology
Raissa Nogueira de Brito, Susan Tanner, Julie Velasquez Runk, Juliana Hoyos
Summary: This study conducted a scoping review to identify major research themes and knowledge gaps in the social science literature on leishmaniases or Chagas disease. The findings showed that research in this field mainly focuses on the sociocultural, economic, historical, and political dimensions of the diseases. Future research should pay more attention to how social institutions and economic factors shape disease education, control measures, healthcare access, and quality of life of affected individuals.
Article
Parasitology
Minghao Zheng, Mengyao Zhang, Hao Li, Shipo Wu, Yuee Zhao, Jinlong Zhang, Yunyue Zhou, Mohamed Boie Jalloh, Kun Zhang, Lina Chen, Zhiqiang Mi, Yong Cui, Lihua Hou
Summary: Rapid and convenient detection of Plasmodium falciparum, the deadliest malaria parasite in Africa, is crucial for malaria eradication efforts in medical resource-limited settings. A CRISPR-based diagnostic method that can detect P. falciparum without nucleic acid extraction was developed and showed high sensitivity and consistency with qPCR test. It can also detect artemisinin-resistant strains and other laboratory-cultured strains. This method has great potential for clinical testing and large-scale community screening in Africa and other regions.
Article
Parasitology
Fabian Correa-Morales, Cassandra Gonzalez-Acosta, David Ibarra-Ojeda, Miguel Moreno-Garcia
Summary: Since 2002, West Nile Virus (WNV) has been reported in 18 states in Mexico. This paper discusses the proposed hypotheses for the absence of WNV outbreaks in Latin America and provides a context-specific explanation for Mexico.
Article
Parasitology
Umberto Molini, Lauren M. Coetzee, Vernon Christians, Maria Y. Hemberger, Bernard Chiwome, Maria Amukwaya, Siegfried Khaiseb, Giovanni Cattoli, William G. Dundon, Giovanni Franzo
Summary: Porcine circovirus 3 (PCV-3) has been reported globally since its first identification in 2015. Limited data on PCV-3 in Africa led to a study in Namibia, which found a high prevalence of PCV-3 in backyard piggeries, but not in commercial farms with higher biosecurity measures. The source of viral introduction and factors such as semen importation, feeding backyard pigs with human food waste, pig exchanges, and wildlife movements may be involved in the dispersal of PCV-3 within Namibia. Further studies are needed to understand the epidemiology and impact of PCV-3 on pig productivity and wildlife health.
Article
Parasitology
Chutchai Piewbang, Panida Poonsin, Pattiya Lohavicharn, Panitnan Punyathi, Sawang Kesdangsakonwut, Tanit Kasantikul, Somporn Techangamsuwan
Summary: This study provides insights into the pathology of natural SARS-CoV-2 infection in dogs, revealing severe damage to the lungs and involvement of multiple organs. The findings suggest the potential utility of dogs as a model for studying SARS-CoV-2 infection in humans, highlighting the need for further investigation.
Article
Parasitology
Julian Avila-Jimenez, Juan David Gutierrez, Mariano Altamiranda-Saavedra
Summary: Leishmaniasis is a parasitic disease transmitted to humans by sandfly vectors and vertebrate hosts. This study used ecological niche modeling to investigate the potential distribution of host and vector species associated with cutaneous leishmaniasis transmission, revealing different distribution patterns under varying climatic conditions.
Article
Parasitology
Luis A. Gomez-Puerta, Juan Olazabal, Santiago Paredes, Juan Lopez, Miguel Anampa, Angelica Mondragon, Ana Vargas-Calla, Hugo Castillo
Summary: This study reports the first detection of Candidatus M. haemolamae infection in vicunas from the Pampa Galeras National Reserve in Peru, using molecular diagnosis. Tick-infested vicunas had a higher likelihood of being infected with Candidatus M. haemolamae compared to tick-free vicunas. Gender and age were not associated with infection.
Article
Parasitology
Berlin Londono-Renteria, Zakaria Seidu, Helena Lamptey, Michael F. Ofori, Lars Hviid, Mary Lopez-Perez
Summary: Heterozygous carriers of HbAS and HbAC have a reduced risk of severe malaria, but not immunity against Plasmodium falciparum infection. Antibody levels against gSG6-P1 peptide in Anopheles mosquito saliva can serve as a serological biomarker for exposure to Plasmodium infection. This study highlights the importance of studying the role of hemoglobinopathies in malaria transmission for improving control interventions.
Article
Parasitology
Imane Belbacha, Meryem El Azzouzi, Rajae Bensghir, Kamal Filali Marhoum, Khadija Hajjout, El Mir Elharti, Khalid Sadki, Hicham Oumzil
Summary: This study investigates the association of three SNPs in the APOBEC3G gene with disease outcomes in Moroccan HIV-1 infected patients. The rs2294367 CG genotype is strongly associated with protection against HIV-1 infection, particularly in older individuals.
Article
Parasitology
Tan Luong, Do Kien Tran, Anh Hung Pham, Thi Thu-Ha Hoang, Van Khang Pham, Quang Thai Pham, Thi Mai Hung Tran, Minh Hoa Luong, Thanh Long Pham, Jason K. Blackburn
Summary: This study examined the epidemiology of anthrax in Lai Chau province, Vietnam, using historical data. Spatial clusters of human and livestock anthrax were identified, and the characteristics of the disease were described. The study found that there were distinct differences between the high-risk areas for human and livestock anthrax, and most human cases occurred during specific seasons. Additionally, the decrease in livestock anthrax vaccine coverage was associated with an increase in disease incidence.
Article
Parasitology
Jin-tao Chen, Jian-bo Zhan, Ming-chao Zhu, Kai-ji Li, Man-qing Liu, Bin Hu, Kun Cai, Hai-rong Xiong, Shu-liang Chen, Wei-long Tan, Liang-jun Chen, Wei Hou
Summary: This study investigated the prevalence and variation of orthohantavirus in rodents and humans in Hubei province, China. It identified the diversity of orthohantaviruses circulating in the region, with the HV004-like subtype being the main genotype in rodents and patients. This highlights the importance of continued attention and research on orthohantaviruses, especially newly identified strains.
