期刊
GENETICS AND MOLECULAR RESEARCH
卷 15, 期 4, 页码 -出版社
FUNPEC-EDITORA
DOI: 10.4238/gmr15048875
关键词
Brazilian red pepper essential oil; Ex vivo; Swine; Intestinal alkaline phosphatase; Intestinal explants; Sodium butyrate
资金
- Science Without Borders Program (CNPq) from the Brazilian government
- Purdue University
- Pontificia Universidade Catolica do Parana (PUCPR)
In this study, we evaluated the effect of intestinal alkaline phosphatase (IAP) and sodium butyrate (NaBu) on lipopolysaccharide (LPS)-induced intestinal inflammation. Intestinal alkaline phosphatase and RelA/p65 (NF-kappa B) gene expressions in porcine jejunum explants were evaluated following exposure to sodium butyrate (NaBu) and essential oil from Brazilian red pepper (EO), alone or in combination with NaBu, as well as exogenous IAP with or without LPS challenge. Five piglets weighing approximately 20 kg each were sacrificed, and their jejunum were extracted. The tissues were segmented into 10 parts, which were exposed to 10 treatments. Gene expressions of IAP and RelA/p65 (NF-kappa B) in jejunal explants were evaluated via RT-PCR. We found that EO, NaBu, and exogenous IAP were able to up-regulate endogenous IAP and enhance RelA/p65 (NF-kappa B) gene expression. However, only NaBu and exogenous IAP down-regulated LPS-induced inflammatory response via RelA/p65 (NF-kappa B). In conclusion, we demonstrated that exogenous IAP and NaBu may be beneficial in attenuating LPS-induced intestinal inflammation.
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