Article
Plant Sciences
Haiyan Ma, Xiaoying Wu, Ziwang Wei, Liang Zhao, Zhongze Li, Qing Liang, Jie Zheng, Yu Wang, Yanhua Li, Linfei Huang, Qiang Hu, Danxiang Han
Summary: The study revealed that a type of enzyme in microalgae, DGAT, may lose its original function and instead function as another enzyme, LPAAT. The HpDGTT2 identified in the experiment was found to act as a LPAAT, regulating lipid biosynthesis, which could help to elucidate the structure-function relationship of this group of enzymes.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Yuanxue Liang, Xiao-Hong Yu, Sanket Anaokar, Hai Shi, William B. Dahl, Yingqi Cai, Guangbin Luo, Jin Chai, Yuanheng Cai, Almudena Molla-Morales, Fredy Altpeter, Evan Ernst, Jorg Schwender, Robert A. Martienssen, John Shanklin
Summary: Expression of specific genes in duckweed can increase triacylglycerol (TAG) accumulation, providing a new opportunity for biofuel and lipidic bioproduct production. These findings lay the foundation for optimizing TAG accumulation in duckweed and have the potential to greatly increase oil yield compared to soybean and oil palm.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Yi-Ying Lee, Rudolph Park, Stephen M. Miller, Yantao Li
Summary: Genetic compensation is proposed as a mechanism to explain phenotypic differences between gene knockouts and knockdowns. This study demonstrates examples of genetic compensation in microalgae and suggests its physiological relevance in TAG biosynthesis under stress. These findings provide guidance for future genetic engineering and mutant characterization efforts.
Article
Multidisciplinary Sciences
Zhenying Peng, Ling Zheng, Haiying Tian, Jianguo Wang, Wenwen Liu, Jingjing Meng, Jialei Zhang, Xinguo Li, Shubo Wan
Summary: Three amino acid mutations, T107M, K251R, and L316P, affect the activity of Arachis hypogaea diacylglycerol acyltransferase 2. T107M and K251R alter the fatty acid content and composition in transformed yeast strains, while L316P leads to the loss of enzyme activity.
Article
Plant Sciences
Sizhang Liu, Yue Jiang, Yanfang Wang, Huimin Huo, Mustafa Cilkiz, Ping Chen, Yilai Han, Li Li, Kangyu Wang, Mingzhu Zhao, Lei Zhu, Jun Lei, Yi Wang, Meiping Zhang
Summary: In this study, the genetic and molecular characteristics of ginseng were analyzed, resulting in the development of a mini-core collection representing the genetic variation of ginseng. Transcriptome sequencing revealed the wide molecular diversity of ginseng, and analysis of ginsenoside content showed extensive variation and correlation. The relationships between cultivars and landraces were also examined in terms of gene expression, gene variation, and ginsenoside biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Weizong Yang, Ziwei Xin, Lihang Xie, Yuhui Zhai, Yanlong Zhang, Lixin Niu, Qingyu Zhang
Summary: This study investigated the molecular mechanisms underlying the high accumulation of alpha-linolenic acid (ALA) in tree peony seeds. The results showed that triacylglycerol (TAG) lipid molecules, especially ALA, increased in abundance and diversity during seed development. The study also identified genes involved in the transfer of fatty acids to the TAG sn-3 position. These findings provide functional evidence for increasing ALA yield in tree peony and other oilseed crops.
HORTICULTURE RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Paeka Klaitong, Akaraphol Watcharawipas, Sirirat Fa-aroonsawat, Wipa Chungjatupornchai
Summary: This study investigated the regulation of diacylglycerol acyltransferase 2 gene (NeoDGAT2) in microalgae by analyzing the regulatory sequence RDG, revealing the potential role of MYB transcription factor in controlling TAG biosynthesis. The interaction between MYB transcription factor and MYB-bs may play a crucial role in regulating -N induced expression of NeoDGAT2 and affecting TAG accumulation, making MYB transcription factors a potential target for engineering to increase TAG content for economic viability.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kaori Oyama, Izumi Matsuwaki, Maika Ito, Rei Iwahori, Haruka Nagata, Isuzu Nakamura, Ayaka Kondo, Akane Kodaka, Yurika Fuseya, Haruka Yamamoto, Yui Ueyama, Yoko Ide, Yuki Kasai, Shigeaki Harayama, Misako Kato
Summary: In this study, the de novo synthesis of triacylglycerol (TAG) in Coccomyxa sp. Obi strain under nitrogen (N)-replete and N-limiting conditions was analyzed. The results showed that TAG synthesis is limited by fatty acid supply under N-replete conditions, while it is enhanced under N-limiting conditions. The expression of the acyl-ACP thioesterase, FAT1, involved in releasing fatty acids from acyl-ACP, was induced under N-limiting conditions. Furthermore, the turnover of TAG was significant under N-replete conditions. The slow conversion of diacylglycerol to TAG partly explained the slower rate of TAG synthesis compared to polar lipid synthesis under N-limiting conditions.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Chemistry, Applied
Changfeng Hu, Jingyi Song, Wumeng Jin, Wenqiang Wang, Hongjin Bai, Cuiyun Wu, Lirong Shen
Summary: Walnut oil, rich in polyunsaturated fatty acids, has many health benefits. The synthesis and accumulation of walnut oil in the embryo development process may be influenced by a special mechanism for triacylglycerol biosynthesis. Shotgun lipidomics analysis revealed that triacylglycerol synthesis in walnut kernels occurred before 84 days after flowering and was enhanced between 84 and 98 days. Acyl editing through phosphatidylcholine played a role in the flux of fatty acids for triacylglycerol synthesis. Therefore, triacylglycerol biosynthesis in walnut kernels is directly linked to lipid metabolism.
Article
Chemistry, Medicinal
Eun-Bin Kwon, Dong-Oh Moon, Eun Sol Oh, Yu Na Song, Ji-Yoon Park, Hyung Won Ryu, Doo-Young Kim, Young-Won Chin, Hyun-Sun Lee, Su Ui Lee, Mun-Ock Kim
Summary: This study found that the extract of Garcinia mangostana peel can inhibit the activities of diacylglycerol acyltransferase (DGAT) and pancreatic lipase (PL), which could be beneficial in the treatment of TG-related metabolic diseases by suppressing TG absorption and synthesis.
JOURNAL OF MEDICINAL FOOD
(2023)
Article
Plant Sciences
Nina Alyssa M. Barroga, Yuki Nakamura
Summary: LPAT2 is a crucial catalyst involved in phospholipid synthesis and plant development in Arabidopsis, with different effects in different organs.
Article
Forestry
Xue Bai, Yongyi Yang, Lun Xie, Qingqing Li, Biao Xiong
Summary: In this study, 15 LgDGAT family genes were identified and analyzed in Lindera glauca. The gene structure, chromosome distribution, subcellular localization, promoter prediction, phylogenetic relationships, tissue-specific expression, and expression patterns during fruit development were comprehensively investigated. The findings provide insights into the functionality and potential roles of DGAT genes in lipid metabolism and oil accumulation in Lindera species.
Article
Biochemistry & Molecular Biology
Bowei Yan, Chuanyi Chang, Yingnan Gu, Nan Zheng, Yuyan Fang, Ming Zhang, Guijiang Wang, Liguo Zhang
Summary: This study characterized the Cannabis sativa DGAT gene family, which is associated with important processes like growth, environmental adaptation, and stress responses. The expression dynamics of CsDGAT genes vary in different tissues and varieties, suggesting distinct functional roles in cold responses and shaping different parts of hemp seedlings' responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Karuppaiyan Jothibasu, D. W. Dhar, Suchitra Rakesh
Summary: Microalgae based biofuel has been extensively researched for over a decade, with a focus on optimizing lipid accumulation and recovery for economical production; Strain improvement through genetic engineering is key for the commercialization of microalgae as feedstocks for carbon-neutral biofuels; Future efforts should address continuous oil production and non-destructive recovery for large scale commercial biofuel production.
BIOMASS & BIOENERGY
(2021)
Article
Immunology
Zhenzhou Huang, Keyi Yu, Ruiting Lan, J. Glenn Morris, Yue Xiao, Julian Ye, Leyi Zhang, Longze Luo, He Gao, Xuemei Bai, Duochun Wang
Summary: This study describes the whole-genome features of V. metschnikovii strains and identifies two major lineages and 29 O-genotypes. Moreover, it identifies 19 virulence-associated factors and highlights the importance of lateral flagella in the early stages of infection.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Ecology
Gabriela de Carvalho Fernandes, Andreia Carina Turchetto-Zolet, Luciane Maria Pereira Passaglia
Summary: Glutamine synthetase (GS) is an essential enzyme in nitrogen assimilation. Studies have revealed that GS-encoding genes are among the oldest functioning genes and have a complex evolutionary history involving gene duplications and transfers. GS-encoding genes can be classified into three distinct groups, with the Type I family being the most diverse and including subgroups related to polyamine metabolism and noncatalytic GS homologs.
Article
Biochemistry & Molecular Biology
Natalia Balbinott, Nureyev Ferreira Rodrigues, Frank Lino Guzman, Andreia Carina Turchetto-Zolet, Rogerio Margis
Summary: This study investigates the phylogenetic relationships of Myrtaceae tribes using plastid protein-coding sequences and nuclear markers. The results differ from previous analyses and redefine certain branch relationships.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Editorial Material
Plant Sciences
Clarisse Palma-Silva, Andreia Carina Turchetto-Zolet, Michael F. Fay, Thais Vasconcelos
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Plant Sciences
Nicole Moreira Veto, Dragos Postolache, Frank L. Guzman Escudero, Elia Vajana, Ricardo Burgo Braga, Fabiano Salgueiro, Rogerio Margis, Giovanni G. Vendramin, Andreia C. Turchetto-Zolet
Summary: This study investigates population genetic structure and adaptive genetic diversity in Eugenia uniflora, a tropical tree species. The study finds spatial population structuring and higher genetic diversity in southern populations. It also identifies candidate loci potentially involved in local adaptation.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2023)
Review
Plant Sciences
Caroline Turchetto, Ana Lucia A. Segatto, Andreia C. Turchetto-Zolet
Summary: The coevolution between floral traits and pollination behavior plays a significant role in the diversification of angiosperms. Hybridization is also found to occur between plants with specialist pollination syndromes. Pollinators, such as bees and hummingbirds, are likely to be the primary vectors of gene flow among different plant species, leading to the formation of hybrid zones. Understanding the role of pollinators in plant diversification and the factors influencing hybridization is crucial, especially in megadiverse regions like the Neotropics.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Agronomy
Karolline R. Silva, Sarah K. O. Cabral, Mateus B. de Freitas, Aline C. Velho, Rogerio Margis, Marciel J. Stadnik, Franceli R. Kulcheski
Summary: The co-evolution of plant pathosystems has resulted in the development of molecular mechanisms that enable plants to defend themselves against invading pathogens. This study investigated the expression profile of DRB genes in Arabidopsis thaliana in response to eukaryotic pathogens of different lifestyles. The results provide the first evidence linking DRB genes to the response of plants to fungi and oomycetes.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Isabel Cristina Cadavid, Natalia Balbinott, Rogerio Margis
Summary: Plants have developed multiple mechanisms to cope with environmental variations, such as physiological, morphological, and molecular adaptations. Epigenetic regulation and post-transcriptional regulation operated by RNA molecules are important mechanisms associated with gene expression regulation under stress. The review focuses on recent progress in soybean gene expression regulation through epigenetic modifications and circRNAs pathways, and highlights the potential of circular RNA in post-transcriptional processing.
GENETICS AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thomaz Stumpf Trenz, Andreia Carina Turchetto-Zolet, Rogerio Margis, Marcia Margis-Pinheiro, Felipe dos Santos Maraschin
Summary: In this study, the authors functionally characterized DGATs in castor beans and found that RcDGAT3 is localized in vesicles outside the ER in leaf epidermal cells and provides higher tolerance over FFA. RcDAcTA is associated with the ER membranes but fails to rescue long-chain TAG biosynthesis. These findings suggest alternative functions and roles of RcDGAT3 in lipid metabolism.
GENETICS AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Douglas Jardim-Messeder, Andreia Caverzan, Gabriel Afonso Bastos, Vanessa Galhego, Ygor de Souza-Vieira, Fernanda Lazzarotto, Esther Felix-Mendes, Lucas Lavaquial, Jose Nicomedes, Marcia Margis-Pinheiro, Gilberto Sachetto-Martins
Summary: In this study, APX genes were identified from various Poaceae species, and it was found that the APX gene family underwent duplication events and divergence. MitAPX, a unique type in Poaceae, is essential for rice plant growth, while sAPX and tAPX are responsible for regulating hydrogen peroxide levels in the cell and chloroplast, respectively.
GENETICS AND MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Douglas Jardim-Messeder, Andreia Caverzan, Natalia Balbinott, Paloma K. Menguer, Ana L. S. Paiva, Moaciria Lemos, Juliana R. Cunha, Marcos L. Gaeta, Miguel Costa, Marcel Zamocky, Nelson J. M. Saibo, Joaquim A. G. Silveira, Rogerio Margis, Marcia Margis-Pinheiro
Summary: This study found that silencing the OsAPX7 gene in rice does not affect plant growth, redox state, or photosynthesis parameters. However, silenced plants (APX7i) show higher tolerance to drought stress, with a higher ability to maintain stomata aperture and photosynthesis performance compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 does not alter the global expression of genes related to reactive oxygen species metabolism. Additionally, the drought-induced regulation of genes related to proteasome pathway, nitrogen metabolism, and carotenoid metabolism is impaired in APX7i plants. On the other hand, genes associated with flavonoid and tyrosine metabolism are upregulated, while genes related to phytohormone signal transduction and nicotinate and nicotinamide metabolism are downregulated in APX7i during drought stress. These results suggest that OsAPX7 may be involved in signaling transduction pathways related to drought stress response in rice, providing insights into the physiological role of chloroplast APX isoforms.
Article
Multidisciplinary Sciences
Drielli Canal, Frank Lino Guzman Escudero, Luiza Alves Mendes, Marcia Flores da Silva Ferreira, Andreia Carina Turchetto-Zolet
Summary: Terpenoids play essential roles in plant growth, defense, and adaptation. This study investigated the terpene synthase gene (TPS) family in two morphotypes of Psidium cattleyanum and found different expression patterns and oil compositions, suggesting distinct gene regulation mechanisms. The study also identified a lineage-specific subfamily expansion and amino acid residues under positive selection, providing insights into terpene biosynthesis and their potential involvement in adaptation mechanisms in Neotropical Myrtaceae species.
SCIENTIFIC REPORTS
(2023)
Correction
Plant Sciences
Nicole Moreira Veto, Dragos Postolache, Frank L. Guzman Escudero, Elia Vajana, Ricardo Burgo Braga, Fabiano Salgueiro, Rogerio Margis, Giovanni G. Vendramin, Andreia C. Turchetto-Zolet
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2023)
