期刊
GENETICS AND MOLECULAR BIOLOGY
卷 39, 期 1, 页码 62-72出版社
SOC BRASIL GENETICA
DOI: 10.1590/1678-4685-GMB-2014-0300
关键词
Auxin transporter; PvPIN1 gene; TIBA treatment; tillering; switchgrass
资金
- Natural Science Foundation of China [31171607, 31371690]
Switchgrass (Panicum virgatum L.; family Poaceae) is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, including the regulation of shoot and root branching, plant resistance and biological yield. Auxin transport and gradients in plants are mediated by influx and efflux carriers. PvPIN1, a switchgrass PIN1-like gene that is involved in regulating polar transport, is a putative auxin efflux carrier. Neighbor-joining analysis using sequences deposited in NCBI databases showed that the PvPIN1 gene belongs to the PIN1 family and is evolutionarily closer to the Oryza sativa japonica group. Tiller emergence and development was significantly promoted in plants subjected to PvPIN1 RNA interference (RNAi), which yielded a phenotype similar to that of wild-type plants treated with the auxin transport inhibitor TIBA (2,3,5-triiodobenzoic acid). A transgenic approach that induced PvPIN1 gene overexpression or suppression altered tiller number and the shoot/root ratio. These data suggest that PvPIN1 plays an important role in auxin-dependent adventitious root emergence and tillering.
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