Article
Veterinary Sciences
Mariana M. M. Rezende-Gondim, Aristeu V. V. da Silva, Jitender P. P. Dubey, Gereon R. M. Schares, Luis F. P. Gondim
Summary: This study describes an immunomagnetic separation (IMS) method that effectively purifies T. gondii tissue cysts generated in cell culture, facilitating proteomic studies of these cysts.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Parasitology
Marcus Truong, Jan Slapeta
Summary: Researchers optimized single-plex probe-based qPCR assays into a multiplexed qPCR panel to detect cyst-forming coccidia, specifically T. gondii and N. caninum. The multiplexed qPCR assay showed a high level of analytical sensitivity, with a limit of detection of 100 copies of target sequence synthetic DNA. This assay can be used for effective detection of parasite DNA in animal tissue for diagnostic purposes.
PARASITOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Sarah L. Sokol-Borrelli, Sarah M. Reilly, Michael J. Holmes, Stephanie B. Orchanian, Mackenzie D. Massmann, Katherine G. Sharp, Leah F. Cabo, Hisham S. Alrubaye, Bruno Martorelli Di Genova, Melissa B. Lodoen, William J. Sullivan Jr, Jon P. Boyle
Summary: The identification of the ROCY1 gene as a regulator of T. gondii bradyzoite formation provides insights into the long-term persistence of the parasite. It is not required for parasite reactivation, raising questions about the persisting life stage responsible for causing recrudescent disease.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Matthew L. Blank, Jing Xia, Mary M. Morcos, Mai Sun, Pamela S. Cantrell, Yang Liu, Xuemei Zeng, Cameron J. Powell, Nathan Yates, Martin J. Boulanger, Jon P. Boyle
Summary: Research identified interactions between a single parasite effector and multiple target host proteins at the interface between the Toxoplasma gondii vacuole and the host mitochondria, some of which are critical for the HMA phenotype itself.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agriculture, Dairy & Animal Science
Helga Waap, Andrea Baerwald, Telmo Nunes, Gereon Schares
Summary: This study evaluated the seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in naturally exposed cattle in Portugal. The results showed a low but widespread seroprevalence of T. gondii, while N. caninum had a seroprevalence similar to other Mediterranean countries. Climate factors and management practices were significant predictors of infection for both parasites. The study highlights the importance of considering neosporosis in the differential diagnosis of cattle reproductive disorders and informing source attribution models for human toxoplasmosis.
Article
Biochemistry & Molecular Biology
Luisa Berna, Pablo Marquez, Andres Cabrera, Gonzalo Greif, Maria E. Francia, Carlos Robello
Summary: The study reveals errors in the assembly of the Neospora caninum genome and significant chromosomal rearrangements between Neospora caninum and Toxoplasma gondii, leading to a reduction in their karyotype. Additionally, more than 500 new genes were identified through reannotation of the Neospora caninum genome.
Article
Veterinary Sciences
Qiu-Yan Lv, He-Liang Zheng, Wen-He Yang, Guo-Hua Liu
Summary: The study investigated the genotypes of Toxoplasma gondii and Neospora caninum in domestic ducks in Hunan province, China. Results showed low infection rates and highlighted the importance of a One Health approach to managing toxoplasmosis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Lei Ma, Shuai Li, Yongbin Zhang, Ziru Wen
Summary: The study revealed a higher seroprevalence of N. caninum in dairy cows in Hebei province, China, compared to previous data, and it is the first to detect T. gondii and N. caninum antibodies in the vast areas of Hebei province.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eva Bartova, Jirina Markova, Jana Sedlackova, Hana Band'ouchova, Karol Racka
Summary: In this study, wild bats were tested for the presence of selected parasites. The results showed that Toxoplasma gondii was detected in 1% of bats, while Neospora caninum was not found in any of the bats. Encephalitozoon spp. was detected in 25% of bats, including three different species. This is the first study to report a relatively high positivity of Encephalitozoon spp. in vespertilionid bats from Central Europe and worldwide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jamal Gharekhani, Mohammad Yakhchali, Asghar Afshari, Maryam Adabi
Summary: The study conducted a comprehensive evaluation of Neospora, Toxoplasma, and Brucella infections in milk samples from dairy farms in Hamedan province, Iran for the first time. Results showed that milk quality was affected by infections. Control measures such as regulating animal age, herd size, and animal species, as well as health education and milk pasteurization, can help reduce the spread of infectious diseases.
Review
Immunology
Ragab M. Fereig, Mosaab A. Omar, Abdullah F. Alsayeqh
Summary: Toxoplasmosis and neosporosis are important protozoan diseases worldwide, and controlling these diseases is currently challenging. Understanding host-parasite interactions and host strategies to combat infections can aid in developing effective control measures, including vaccines. Macrophages and the production of interleukin-12 (IL-12) and interferon gamma (IFN-gamma) play a crucial role in successful vaccine candidates. However, identifying potential vaccine candidates is a time-consuming process.
Article
Agriculture, Dairy & Animal Science
Ragab M. Fereig, Gamal Wareth, Hanan H. Abdelbaky, Amira M. Mazeed, Mohamed El-Diasty, Adel Abdelkhalek, Hassan Y. A. H. Mahmoud, Alsagher O. Ali, Abdelrahman El-tayeb, Abdullah F. Alsayeqh, Caroline F. Frey
Summary: Toxoplasmosis, neosporosis, and brucellosis are common diseases causing infectious abortion and economic losses in farm animals. The study in Egypt found the highest seroprevalence rate for T. gondii in sheep and goats, with only antibodies to Brucella spp. associated with recent abortion. These findings could guide the development of more efficient control measures.
Article
Agriculture, Dairy & Animal Science
Jinfang Yang, Jingkai Ai, Tongsheng Qi, Xiaomin Ni, Zichun Xu, Liangting Guo, Yali Sun, Ying Li, Ming Kang, Jixu Li
Summary: This study establishes a record of the seroprevalence and molecular epidemiology of T. gondii and N. caninum infection in stray cats and dogs in the Qinghai-Tibetan Plateau Area. The results demonstrate the presence of both parasites in the tested animals and highlight the importance of stray cats and dogs in the transmission and prevalence of these infections.
Article
Microbiology
Stefania Zanet, Fabrizia Veronesi, Giuseppe Giglia, Carolina Raquel Pinto Baptista, Giulia Morganti, Maria Teresa Mandara, Rachele Vada, Luis Manuel Madeira De Carvalho, Ezio Ferroglio
Summary: The prevalence of cyst-forming parasites Toxoplasma gondii and Neospora caninum was assessed in migratory and sedentary birds of prey. Molecular and histopathological analysis were conducted on skeletal muscle and myocardium samples from 159 birds of prey from Central Italy, revealing a prevalence of 23.27% for T. gondii and 5.66% for N. caninum.
Article
Microbiology
Dina B. Salama, Ragab M. Fereig, Hanan H. Abdelbaky, Moshera S. Shahat, Waleed M. Arafa, Shawky M. Aboelhadid, Adel E. A. Mohamed, Samy Metwally, Osama Abas, Xun Suo, Nishith Gupta, Caroline F. Frey
Summary: This study investigated the infection rates of Toxoplasma gondii and Neospora caninum in dogs and cats in Egypt. The results showed that 23.3% of dogs were exposed to T. gondii, while 5.8% had N. caninum-specific antibodies. In cats, the exposure rate to T. gondii was 9.8%, and 3.4% had N. caninum-specific antibodies. Factors such as age and breed were significantly associated with T. gondii antibody positivity in dogs.
Article
Cell Biology
Lena Pernas, Camilla Bean, John C. Boothroyd, Luca Scorrano
Article
Biochemistry & Molecular Biology
Matthew L. Blank, Michelle L. Parker, Raghavendran Ramaswamy, Cameron J. Powell, Elizabeth D. English, Yaw Adomako-Ankomah, Lena F. Pernas, Sean D. Workman, John C. Boothroyd, Martin J. Boulanger, Jon P. Boyle
MOLECULAR MICROBIOLOGY
(2018)
Article
Immunology
Nicholas Hernandez, Isabelle Melki, Huie Jing, Tanwir Habib, Susie S. Y. Huang, Jeffrey Danielson, Tomasz Kula, Scott Drutman, Serkan Belkaya, Vimel Rattina, Lazaro Lorenzo-Diaz, Anais Boulai, Yoann Rose, Naoki Kitabayashi, Mathieu P. Rodero, Cecile Dumaine, Stephane Blanche, Marie-Noelle Lebras, Man Chun Leung, Lisa Sara Mathew, Bertrand Boisson, Shen-Ying Zhang, Stephanie Boisson-Dupuis, Silvia Giliani, Damien Chaussabel, Luigi D. Notarangelo, Stephen J. Elledge, Michael J. Ciancanelli, Laurent Abel, Qian Zhang, Nico Marr, Yanick J. Crow, Helen C. Su, Jean-Laurent Casanova
JOURNAL OF EXPERIMENTAL MEDICINE
(2018)
Article
Multidisciplinary Sciences
Jeffrey J. Danielson, Nicolas Perez, Julia D. Romano, Isabelle Coppens
Article
Microbiology
Elizabeth D. English, Jon P. Boyle
Article
Immunology
David Buchbinder, Ivan Kirov, Jeffrey Danielson, Nirali N. Shah, Alexandra F. Freeman, Rishikesh S. Chavan, Helen C. Su
JOURNAL OF CLINICAL IMMUNOLOGY
(2019)
Editorial Material
Microbiology
Lena Pernas
Article
Biochemistry & Molecular Biology
Elizabeth D. English, Boris Striepen
Article
Microbiology
Jayesh Tandel, Elizabeth D. English, Adam Sateriale, Jodi A. Gullicksrud, Daniel P. Beiting, Megan C. Sullivan, Brittain Pinkston, Boris Striepen
NATURE MICROBIOLOGY
(2019)
Article
Immunology
Rachel S. Coombs, Matthew L. Blank, Elizabeth D. English, Yaw Adomako-Ankomah, Ifeanyi-Chukwu Samuel Urama, Andrew T. Martin, Felix Yarovinsky, Jon P. Boyle
INFECTION AND IMMUNITY
(2020)
Editorial Material
Parasitology
Elizabeth D. English, B. Joanne Power, Elena Gomez-Diaz
TRENDS IN PARASITOLOGY
(2020)
Review
Cell Biology
Lena Pernas
Summary: The study discusses the resistance of host cell metabolism against pathogens and highlights how rewiring metabolic processes can enhance cellular defenses and hinder microbial growth.
JOURNAL OF CELL SCIENCE
(2021)
Review
Microbiology
Tania C. Medeiros, Chahat Mehra, Lena Pernas
Summary: Intruding microbes can occupy the host cytosol and interact with host organelles, especially mitochondria. Despite the crucial role of mitochondria in cellular metabolic balance and mediating responses to microbial infections, there is limited understanding of how mitochondria interact with intracellular pathogens and how these interactions impact disease pathogenesis.
CURRENT OPINION IN MICROBIOLOGY
(2021)