Article
Biochemistry & Molecular Biology
Yen Teng Tai, Tomoyuki Fukuda, Yuichi Morozumi, Hayato Hirai, Arisa H. Oda, Yoshiaki Kamada, Yutaka Akikusa, Tomotake Kanki, Kunihiro Ohta, Kazuhiro Shiozaki
Summary: TORC1 is activated in response to nutrient availability and growth factors, promoting cellular anabolism and proliferation. This study revealed that TORC1 phosphorylates and protects the transcription factor Sfp1, which then positively regulates ribosome production genes together with Ifh1 and Fhl1. The transcriptional regulation of ribosome biosynthesis genes by Sfp1, Ifh1, and Fhl1 is one of the key pathways through which nutrient-activated TORC1 promotes cell proliferation.
MOLECULAR AND CELLULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Daniel Williamson, William Tasker-Brown, James A. H. Murray, Angharad R. Jones, Leah R. Band
Summary: This article investigates the mechanisms of cell-size control in plant cells and establishes a mathematical model to simulate the key interactions in the cell cycle. The study finds that both size-independent synthesis and equal inheritance of cell-cycle proteins contribute to cell-size control, but combining these mechanisms at the two key transitions in the cell cycle is essential in maintaining cell-size homeostasis.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Patricia Garcia, Ruben Celador, Jorge Perez-Parrilla, Yolanda Sanchez
Summary: This article focuses on the role of fission yeast Rho1p GEFs in maintaining the shape and homeostasis of yeast cells. These proteins activate Rho1p in different ways and at different times, and also have noncanonical roles in genomic instability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Claudia Salat-Canela, Merce Carmona, Rebeca Martin-Garcia, Pilar Perez, Jose Ayte, Elena Hidalgo
Summary: Cdc42 GTPase regulates cell polarity and growth in fission yeast through positive and negative regulation by GAPs and GEFs. Oxidative stress triggers Sty1 kinase-dependent inactivation of Cdc42 at cell poles, affecting cell morphology. The phosphorylation of Rga3/6 and Gef1 by Sty1 is proposed to mediate Sty1-dependent inhibition.
Review
Biochemistry & Molecular Biology
Ken Ishikawa, Shigeaki Saitoh
Summary: Isolation and introduction of genetic mutations is a powerful approach to characterize gene functions in model yeasts, but not feasible for all genes. To overcome this difficulty, conditional and partial repression of target transcription is possible. This review summarizes gene perturbation technologies, including recent advances in methods based on CRISPR-Cas systems for Schizosaccharomyces pombe, and discusses how CRISPRi can promote fission yeast genetics.
Review
Cell Biology
Claudia Salat-Canela, Pilar Perez, Jose Ayte, Elena Hidalgo
Summary: General stress responses aim to promote cell survival and fitness under adverse conditions. In fission yeast, the activation of MAP kinase cascades can transiently inhibit polarized cell growth through the phosphorylation of Cdc42 regulators.
TRENDS IN CELL BIOLOGY
(2023)
Article
Plant Sciences
Yoshimi Nakano, Hitoshi Endo, Lorenz Gerber, Chiaki Hori, Ayumi Ihara, Masayo Sekimoto, Tomoko Matsumoto, Jun Kikuchi, Misato Ohtani, Taku Demura
Summary: Researchers have developed a self-reinforced system to enhance the formation of secondary cell walls (SCW) in poplar xylem. By using transcription factors fused with specific gene promoters, the expression levels of SCW-related genes were increased, resulting in enhanced SCW thickening in transgenic plants. Furthermore, this method was found to affect the properties of cell walls.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yanna Shi, Julia Vrebalov, Hui Zheng, Yimin Xu, Xueren Yin, Wenli Liu, Zimeng Liu, Iben Sorensen, Guanqing Su, Qiyue Ma, Daniel Evanich, Jocelyn K. C. Rose, Zhangjun Fei, Joyce Van Eck, Theodore Thannhauser, Kunsong Chen, James J. Giovannoni
Summary: Fruit softening is a key aspect of irreversible ripening, and the SlLOB1 transcription factor plays a role in regulating cell wall dynamics and softening, impacting the expression of cell wall-related genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Takamasa Fukunaga, Takao Ohashi, Yutaka Tanaka, Tomoki Yoshimatsu, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
Summary: Schizosaccharomyces japonicus requires Gal-containing oligosaccharides for cell wall integrity during filamentous growth. The cell-surface of S. japonicus primarily consists of tri-saccharides (Gal-Man-Man), unlike other species. Disruption of the UDP-Gal transporter gene results in a complete lack of Gal on the cell surface and blocks hyphal development.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Plant Sciences
Jiewei Shi, Qunxia Zhang, Xu Yan, Delin Zhang, Qin Zhou, Yuxiao Shen, Nachaisin Anupol, Xiuqing Wang, Manzhu Bao, Robert M. Larkin, Hong Luo, Guogui Ning
Summary: Research indicates that injury induces the expression of genes related to cell wall biosynthesis and cell cycle, with AtMYB46 and RrMYB18 playing important roles in promoting cell wall biosynthesis and cell cycle progression, resulting in abnormal leaf development.
Article
Microbiology
Ana Belen Sanz, Sonia Diez-Muniz, Jennifer Moya, Yuliya Petryk, Cesar Nombela, Jose M. Rodriguez-Pena, Javier Arroyo
Summary: Conditions altering the yeast cell wall lead to the activation of an adaptive transcriptional response mainly governed by the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Two high-throughput screenings were conducted to identify genes related to cell wall integrity and transcriptional program elicited by cell wall stress. Functional groups related to chromatin organization, Golgi vesicle transport, rRNA processing, and protein glycosylation were found to be highly representative. Additionally, genes associated with RNA metabolism, transcription/translation, protein degradation, and chromatin organization were highlighted.
Article
Microbiology
Lingdan Lian, Guang Zhang, Jing Zhu, Yunxiao Wang, Lingshuai Wang, Rui Liu, Liang Shi, Ang Ren, Mingwen Zhao
Summary: This study identified two different transcripts of Swi6, with the isoform GlSwi6B participating in regulating the cell wall integrity signaling in Ganoderma lucidum fungi. GlSwi6B was found to bind to the promoters of chitin and glucan synthesis genes, while this binding phenomenon was not observed in the isoform GlSwi6A.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qi Yin, Wenqi Qin, Zibin Zhou, Ai-Min Wu, Wei Deng, Zhengguo Li, Wei Shan, Jian-ye Chen, Jian-fei Kuang, Wang-jin Lu
Summary: Chilling stress leads to cellulose accumulation in the cell wall, which is caused by the activation of MaCESA proteins. MaNAC1, a cold-inducible NAC transcription factor, plays a vital role in cellulose biosynthesis and enhances cellulose content during low-temperature stress. Our findings highlight the importance of understanding cell wall architecture in biomass production and stress adaptation.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Maribel Marquina, Eva Lambea, Merce Carmona, Marta Sanchez-Marinas, Sandra Lopez-Aviles, Jose Ayte, Elena Hidalgo, Rosa Aligue
Summary: The MAPKAP kinase Srk1 plays a crucial role in a negative feedback loop regulating the Sty1 pathway by phosphorylating and inhibiting the Wis1 MAPKK. This additional regulatory mechanism allows fine-tuning of MAPK signaling for an appropriate stress response.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Jose Cansado, Teresa Soto, Alejandro Franco, Jero Vicente-Soler, Marisa Madrid
Summary: This review provides an overview of the organization and underlying regulatory mechanisms of the Cell Integrity Pathway (CIP) in Schizosaccharomyces pombe, a simple eukaryote. It discusses the diverse functions of this pathway in mRNA stability, calcium homeostasis, cell wall integrity, and cytokinesis. Additionally, it explores the intricate interplay between the CIP and other environmentally regulated pathways. The evolutionary conservation of CIP signaling in the dimorphic fission yeast S. japonicus is also addressed.