Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering

The CD28-Transmembrane Domain Mediates Chimeric Antigen Receptor Heterodimerization With CD28

(2021) Yannick D. Muller et al.   Frontiers in Immunology

The promise and challenge of therapeutic genome editing

(2020) Jennifer A. Doudna   NATURE

CRISPR-engineered T cells in patients with refractory cancer

(2020) Edward A. Stadtmauer et al.   SCIENCE

The Emerging Landscape of Immune Cell Therapies

(2020) Evan W. Weber et al.   CELL

The delivery challenge: fulfilling the promise of therapeutic genome editing

(2020) Joost van Haasteren et al.   NATURE BIOTECHNOLOGY

Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts

(2020) Ivana Indikova et al.   NUCLEIC ACIDS RESEARCH

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

(2020) Tuo Wei et al.   Nature Communications

CAR T Cell Therapy for Solid Tumors: Bright Future or Dark Reality?

(2020) Jessica Wagner et al.   MOLECULAR THERAPY

A New Class of Medicines through DNA Editing

(2019) Matthew H. Porteus   NEW ENGLAND JOURNAL OF MEDICINE

Gene editing for immune cell therapies

(2019) Stefanie R. Bailey et al.   NATURE BIOTECHNOLOGY

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing

(2019) Pin Lyu et al.   NUCLEIC ACIDS RESEARCH

A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing

(2019) Guojun Chen et al.   Nature Nanotechnology

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

(2019) David N. Nguyen et al.   NATURE BIOTECHNOLOGY

Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing

(2018) Romain Rouet et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells

(2018) Thomas Del’Guidice et al.   PLoS One

Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide

(2018) Hong-Xia Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming human T cell function and specificity with non-viral genome targeting

(2018) Theodore L. Roth et al.   NATURE

CRISPR/Cas9-mediated PD-1 disruption enhances human mesothelin-targeted CAR T cell effector functions

(2018) Wanghong Hu et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

(2018) Philippe E. Mangeot et al.   Nature Communications

Therapeutic T cell engineering

(2017) Michel Sadelain et al.   NATURE

Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes

(2017) Brett T Staahl et al.   NATURE BIOTECHNOLOGY

Human antibody-based chemically induced dimerizers for cell therapeutic applications

(2017) Zachary B Hill et al.   Nature Chemical Biology

CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells

(2017) Levi J. Rupp et al.   Scientific Reports

Lentivirus pre-packed with Cas9 protein for safer gene editing

(2016) J G Choi et al.   GENE THERAPY

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

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Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases

(2014) Yujia Cai et al.   eLife

DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors

(2013) Yujia Cai et al.   NUCLEIC ACIDS RESEARCH

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Therapeutic potential of HIV protease-activable CASP3

(2012) Kosuke Miyauchi et al.   Scientific Reports

Protein transduction by pseudotyped lentivirus-like nanoparticles

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Efficient gene targeting mediated by a lentiviral vector-associated meganuclease

(2011) Araksya Izmiryan et al.   NUCLEIC ACIDS RESEARCH

Site-specific Gene Insertion Mediated by a Cre-loxP-carrying Lentiviral Vector

(2010) Gilles Michel et al.   MOLECULAR THERAPY

Production of HIV-1 Integrase Fusion Protein-Carrying Lentiviral Vectors for Gene Therapy and Protein Transduction

(2009) Diana Schenkwein et al.   HUMAN GENE THERAPY