期刊
GENES & DEVELOPMENT
卷 30, 期 13, 页码 1558-1572出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.280222.116
关键词
3 ' end processing; Nrd1; polyadenylation; S. pombe; Seb1; transcription termination
资金
- Medical Research Council [MR/L01632X/1]
- Imperial College High Performance Computing Service
- Natural Sciences and Engineering Research Council of Canada (NSREC)
- Wellcome Trust Senior Investigator Award
- UK Medical Research Council
- National Institute of General Medical Sciences [GM084089]
- Wellcome Trust Research and Career Development Grant [091549]
- Canada Research Chair in Quality of Gene Expression
- Medical Research Council [MC_UP_1102/8, MR/L01632X/1] Funding Source: researchfish
- MRC [MR/L01632X/1, MC_UP_1102/8] Funding Source: UKRI
Termination of RNA polymerase II (RNAPII) transcription is associated with RNA 3' end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via a pathway that requires the Nrd1-Nab3-Sen1 (NNS) complex. Here we show that the Schizosaccharomyces pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3' end processing factors, is enriched at the 3' end of genes, and binds RNA motifs downstream from cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3' untranslated region (UTR) length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3' end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 cotranscriptionally controls alternative polyadenylation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据