Article
Biotechnology & Applied Microbiology
Babak Pakbin, Shaghayegh Pishkhan Dibazar, Samaneh Allahyari, Maryam Javadi, Zahra Amani, Alireza Farasat, Sina Darzi
Summary: In this study, the effects of Saccharomyces boulardii supernatant (SBS) on breast cancer cells were investigated. It was found that SBS can induce apoptosis and suppress survivin gene expression, suggesting its potential as an anticancer drug for breast carcinoma.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Multidisciplinary Sciences
Dzhirgala Shamadykova, Dmitry Y. Panteleev, Nadezhda N. Kust, Ekaterina A. Savchenko, Ekaterina Y. Rybalkina, Alexander Revishchin, Galina Pavlova
Summary: GDNF plays a crucial role in the survival, axonal growth, and formation of new synapses in dopaminergic neurons, making it a potential treatment for neurodegenerative disorders; failed clinical trials may be due to issues with post-translational modifications by producer cells; testing on cells, mouse models, and human cells has shown that mGDNF produced by HEK293 cells has the best neuro-inductive ability.
Article
Multidisciplinary Sciences
Heng Li, Jinming Li, Shengbo Yu, Chengwei Wu, Wei Zhang
Summary: The articular cartilage in the knee joint can be anatomically divided into different regions, with distinct mechanical properties observed among these regions. Histological analysis and the use of constitutive models help to explain the differences in cartilage properties, with the Fung and Ogden models found to be suitable for representing the effects of strain rate on stiffening.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Fangyuan Chen, Kai Ding, Nolan Priedigkeit, Ashuvinee Elangovan, Kevin M. Levine, Neil Carleton, Laura Savariau, Jennifer M. Atkinson, Steffi Oesterreich, Adrian V. Lee
Summary: This study revealed transcriptional heterogeneity in breast cancer cell lines and the significant role of E-cadherin depletion in contributing to the molecular features of invasive lobular breast carcinoma.
Article
Biochemistry & Molecular Biology
Marco Franchi, Konstantinos-Athanasios Karamanos, Concettina Cappadone, Natalia Calonghi, Nicola Greco, Leonardo Franchi, Maurizio Onisto, Valentina Masola
Summary: We investigated the effects of extracellular matrix (ECM) on the behavior of LoVo colorectal cancer cells during invasion. Different substrates and culturing time were used to evaluate the expression of epithelial-to-mesenchymal transition (EMT) markers, matrix-degrading enzymes, and morphological phenotypes of LoVo-S and LoVo-R cells. The results showed that the type of substrate and duration of culturing influenced the aggressiveness and morphological phenotypes of the cells differently, with type I Collagen promoting invasiveness in drug-resistant LoVo-R cells. These findings highlight the importance of considering ECM components and dynamic physical conditions in studying colorectal cancer cells.
Editorial Material
Cell Biology
Christopher Schrecker, Andrew M. Intlekofer
Summary: The study identified a selective requirement for pyruvate dehydrogenase (PDH) activity in T cells and T cell leukemia, but not in hematopoietic stem cells (HSCs) or myeloid leukemia, supporting the idea that cell lineage plays a key role in cancer cell metabolism.
Article
Chemistry, Physical
Z. Q. Xu, Z. L. Ma, Y. Tan, M. Wang, Y. Zhao, X. W. Cheng
Summary: This paper investigates the performance of (VNbTiTa)100-xSix (x=0, 2.5, 5, 10) refractory high-entropy alloys (RHEAs). At room temperature, VNbTiTa RHEA exhibits good yield strength and compressive ductility, while silicon additions enhance the strength but with a weakening effect at higher temperatures. At high temperatures, (VNbTiTa)97.5Si2.5 RHEA demonstrates good room-temperature ductility and yield strength.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Biochemistry & Molecular Biology
Carolina Gouvea de Souza Contatori, Camila Ramos Silva, Saulo de Toledo Pereira, Maria Fernanda Setubal Destro Rodrigues, Arthur Cassio de Lima Luna, Marcia Martins Marques, Martha Simoes Ribeiro
Summary: This study investigated the effects of photobiomodulation therapy (PBM) on melanoma cells. The results showed that red laser therapy inhibited the invasive behavior of pigmented melanoma cells and promoted the invasiveness of amelanotic melanoma cells. In vivo experiments also showed that melanoma-bearing mice treated with red laser had smaller tumors and longer survival.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Cell Biology
Michaela Ferencakova, Andrea Benova, Ivan Raska Jr, Pavel Abaffy, Radek Sindelka, Martina Dzubanova, Eliska Pospisilova, Katarina Kolostova, Tomas Cajka, Ales Paclik, Vit Zikan, Michaela Tencerova
Summary: This study investigated the impact of anticoagulants on the molecular characteristics of human BMSCs during isolation and cultivation. The results showed that the type of anticoagulant did not significantly affect the molecular characteristics and cellular composition of BMSCs, while in vitro cultivation caused significant changes in the transcriptomics of BMSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Environmental
Chenyang He, Yu Guo, Bikash Karmakar, Attalla El -kott, Ahmed Ezzat Ahmed, Ahmed Khames
Summary: Nanotechnology involves utilizing atomic and molecular features to create modern systems, tools, and materials, with potential applications in various fields such as medical diagnostics, food, medicine, and biotechnology. Recent studies have shown promising cytotoxic effects on breast carcinoma cell lines using novel Ag NPs supported chitosan-starch composite functionalized Fe3O4 nanoparticles.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Chuang Zhang, Yuanyuan Tian, Ao Yang, Weihua Tan, Xiaoqing Liu, Wenchao Yang
Summary: This study revealed the anti-tumor effect of propolis on human cutaneous squamous cell carcinoma (CSCC) A431 cells and investigated its potential mechanism. The results showed that propolis ethanol extract inhibited A431 cell proliferation by regulating multiple signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chien-Chu Huang, Shao-Chih Chiu, Shih-Chi Chao, Heng-Yi Liao, Shiao-Pieng Lee, Chun-Chung Huang, Der-Yang Cho
Summary: CBD has a cytotoxic effect on oral cancer cells and induces apoptosis. It also affects the morphology and migration of OEC-M1 cells. ECIS analysis shows that CBD decreases the overall resistance and morphological parameters of the cells in a concentration-dependent manner. The study demonstrates that ECIS is more sensitive, functional, and comprehensive than traditional biochemical assays.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alexander Michael Grandits, Sophie Bromberger, Gerwin Heller, Barbara Andrea Reinoehl, Erwin Tomasich, Klaudia Schossleitner, Anna Sophie Berghoff, Thorsten Fuereder, Rotraud Wieser
Summary: Head and neck squamous cell carcinoma (HNSCC) is a frequent malignancy with a poor prognosis. EVI1, a novel oncogene, promotes cellular proliferation and invasiveness in HNSCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Fengyin Li, Xin Zhao, Yali Zhang, Peng Shao, Xiaoke Ma, William J. Paradee, Chengyu Liu, Jianmin Wang, Hai-Hui Xue
Summary: Precise regulation of coinhibitory receptors is crucial for maintaining immune tolerance and protective immunity. In protein immunization, T-FH cells lacking Tcf1 and Lef1 show abnormal upregulation of CTLA4 and LAG3, impacting B-cell help. Targeting CTLA4 and LAG3 can rectify T-FH cell defects and balance immune responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Claudia Tanja Mierke
Summary: Viscoelasticity plays a crucial role in fundamental biological processes such as cell migration and invasion, and it may serve as a biomarker for assessing cell migratory capacity. Statistical mechanics has the potential to explore the biomechanical basis of viscoelasticity. The study of cellular viscoelasticity is important for identifying and characterizing various diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Cell Biology
Claudia Tanja Mierke
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Claudia Tanja Mierke, Alexander Hayn, Tony Fischer
Summary: Cell migration plays a critical role in physiological and pathological processes. The mechano-phenotype of the cytoskeleton affects the efficiency of cell migration and invasion. PINCH1, a focal adhesion protein, plays a key role in cell mechanics and facilitates 3D motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Polymer Science
Nils Wilharm, Tony Fischer, Alexander Hayn, Stefan G. Mayr
Summary: Biopolymer blends, such as collagen elastin hybrid gels, are advantageous materials with novel properties. In this study, the interaction between collagen and elastin fibers during polymerization was investigated, and it was found that elastin fibers bind in a lateral manner to collagen fibers.
Review
Cell Biology
Claudia Tanja Mierke
Summary: Scientific knowledge in the field of cell biology and mechanobiology heavily relies on cell-based in vitro experiments and models that allow the examination and understanding of specific biological processes in different environments. Cell culture assays are valuable tools for biomedical and biophysical research. The quality of experimental models is critical to advancing our understanding of cell-cell and cell-matrix interactions in tissues and organs.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Editorial Material
Cell Biology
Claudia Tanja Mierke
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Alexander Hayn, Tony Fischer, Claudia Tanja Mierke
Summary: The majority of cancer cell investigations have been conducted in oversimplified 2D in vitro environments. However, there has been a recent trend towards more sophisticated 3D in vitro cell culture models to bridge the gap between 2D and in vivo experiments in the field of cancer cell research. This study focuses on the function of ADAM8 in matrix remodeling and migration of 3D extracellular matrix scaffolds, particularly in the context of breast cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Claudia Tanja Mierke
Summary: Posttranslational proteolytic cleavage, particularly by the ADAMs family, plays a crucial role in regulating the function and lifespan of various proteins. ADAM8, a member of this family, has been implicated in regulating neurogenerative diseases, immune function, and cancer by attenuating nearby proteins' functions in the extracellular membrane leaflet. This review article focuses on the roles of ADAM8, shedding function, matrix degradation, effects on cancer cell mobility, interplay with neighboring cells, and its potential impact on cancer progression. The understanding of ADAM8's regulation and activity is expected to unveil potential drug targets for tailored modification of its proteolytic activity in cancer development and metastasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Frank Sauer, Steffen Grosser, Mehrgan Shahryari, Alexander Hayn, Jing Guo, Juergen Braun, Susanne Briest, Benjamin Wolf, Bahriye Aktas, Lars-Christian Horn, Ingolf Sack, Josef A. Kaes
Summary: Cancer progression is accompanied by changes in genetic and cell properties, leading to alterations in a tumor's mechanical state. The hypothesis suggests that tissue fluidization occurs during cancer progression, causing portions of the tissue to change position. The overview provides insights into how cancer-associated mechanisms can affect a tissue's fluidity and stiffness, and how advanced imaging techniques can be used to distinguish tumors from surrounding healthy tissue. A meta-analysis of clinical studies identifies universal trends and presents a roadmap for assessing a tumor's aggressiveness and metastatic potential based on its mechanical properties.
Editorial Material
Cell Biology
Claudia Tanja Mierke
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Polymer Science
Francisco Drusso Martinez-Garcia, Tony Fischer, Alexander Hayn, Claudia Tanja Mierke, Janette Kay Burgess, Martin Conrad Harmsen
Summary: This article reviews the common techniques used for characterizing hydrogel microarchitectures. It provides a concise description of the underlying principles of each method and summarizes the data obtained from cell-free and cell-loaded hydrogels. The advantages and limitations of each technique are discussed and compared. The information presented in this article is of importance to researchers using hydrogels for cell culture, 3D bioprinting, and other areas of hydrogel-based research.
Meeting Abstract
Biophysics
Claudia Tanja Mierke
BIOPHYSICAL JOURNAL
(2022)