Article
Multidisciplinary Sciences
Jing Liu, Gillian M. Schiralli-Lester, Rosemary Norman, David A. Dean
Summary: Acute Lung Injury/Acute Respiratory Distress Syndrome (ALI/ARDS) is characterized by damaged alveoli and edema accumulation, leading to respiratory failure. Gene delivery of Na+, K+-ATPase beta 1 subunit improves alveolar fluid clearance and barrier function, while MRCK alpha gene delivery enhances epithelial and endothelial barrier integrity for ARDS treatment. This study investigates the therapeutic potential of beta 2 and beta 3 subunits in LPS-induced ALI, and found that they do not show the same benefits in lung injury treatment as the beta 1 subunit, suggesting that improving barrier function may be more beneficial than fluid clearance alone in ALI/ARDS treatment.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Rawad Hodeify, Mohamed Chakkour, Reem Rida, Sawsan Kreydiyyeh
Summary: The Na+/K+ ATPase plays a crucial role in regulating the ionic homeostasis of hepatocytes, and its stimulation by PGE2 is mediated through EP4, PKA, and intracellular calcium pathways.
Article
Pharmacology & Pharmacy
Shuomiao Yin, Meizhu Ding, Long Fan, Xuhua Yu, Ziyao Liang, Lei Wu, Zhiling Gao, Lin Lin, Yuanbin Chen
Summary: The total flavonoids extracted from Nervilia fordii (TFENF) protect against LPS-induced ALI by reducing inflammatory cytokines and regulating the active transport capacity of AQPs/ENaCs/Na+-K+-ATPase. These findings suggest the potential therapeutic use of TFENF as a phytomedicine for treating inflammation and pulmonary edema in ALI.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Haixiong Tang, Zemin Chen, Sudan Gan, Yan Liang, Hailing Zhang, Changyun Yang, Liqin Lin, Yubiao Guo, Shiyue Li, Jing Li, Lihong Yao
Summary: This study demonstrates the crucial role of glucose transporter 1 (GLUT1) in the dysfunction of epithelial tight junctions during the development of acute lung injury (ALI).
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Makoto Hosoya, Tsubasa Kitama, Kaho Iwabu, Takanori Nishiyama, Naoki Oishi, Hideyuki Okano, Hiroyuki Ozawa
Summary: Studying the development of the stria vascularis is important for understanding cochlear development in humans and finding new treatment strategies for hearing loss. This study shows that the development of the stria vascularis in the common marmoset is similar to that in humans.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Entomology
Marlena Herbertz, Jennifer Lohr, Christian Lohr, Susanne Dobler
Summary: The study explores the importance of beta-subunits in cell functionality and their tissue-specific distribution. The findings reveal that knockdown of different beta-subunits significantly disrupts molting and results in weakened phenotypes. Furthermore, the study suggests the existence of standalone beta-subunits and their structure-specific distribution, indicating potential pump-independent functions.
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Correction
Biochemistry & Molecular Biology
Mengdie Chen, Yuyu Liu, Shanbai Xiong, Moucheng Wu, Bin Li, Zheng Ruan, Xiaobo Hu
Summary: This study investigated the effect of dietary L-tryptophan on the regulation of tight junctions in LPS-induced intestinal barrier injury in a Caco-2 cell monolayer model.
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Lucy Kate Ladefoged, Birgit Schiott, Natalya U. Fedosova
Summary: The study reveals significant differences in the binding properties of different subfamilies of cardiotonic steroids with the Na+,K+-ATPase. While bufadienolides show beneficial effects with K+, they differ from cardenolides which have an antagonistic relationship with K+. Through simulations, it was found that the binding of bufadienolides is influenced by amino acid coordination patterns and the presence of cations, illustrating the complex interplay of factors in these interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)