Article
Multidisciplinary Sciences
Bo Gao, Liang Liang, Lu Su, Aijia Wen, Chun Zhou, Yu Feng
Summary: Bacterial RecA protein forms filaments with DinI protein in response to DNA damage. These RecA filaments stimulate the autocleavage of LexA, UmuD, and CI, leading to the activation of SOS response and mutagenic repair and lytic cycle. Our study on the structures of RecA filaments in complex with DinI, LexA, UmuD, and CI provides insights into the recognition of conserved protein features in the filament groove, which could be targeted for rational drug design against bacterial SOS response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Alexander Yakimov, Irina Bakhlanova, Dmitry Baitin
Summary: Antibiotic resistance is acquired through activating SOS-dependent mutagenesis and horizontal gene transfer pathways, with compounds that inhibit SOS response being crucial for blocking mutagenesis. Regulators of homologous recombination are potential targets for inhibition, as highlighted in this review.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Vaishali Kaushik, Monalisa Tiwari, Vishvanath Tiwari
Summary: This article discusses the mechanisms of action for antibiotics and how bacteria tolerate DNA damage through SOS response. Additionally, it explores the interconnection between DNA damage repair pathways and other survival mechanisms in bacteria, highlighting the potential of targeting SOS response for therapeutic purposes. The article emphasizes the importance of RecA as a promising target for hindering bacterial SOS response and enhancing antibiotic efficacy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
S. Diaz-Diaz, E. Recacha, J. Machuca, A. Garcia-Duque, F. Docobo-Perez, J. Blazquez, A. Pascual, J. M. Rodriguez-Martinez
Summary: Suppression of the recA gene and overproduction of ROS separately enhance fluoroquinolone activity and lethality. Synergistic effects were observed with double knockouts of recA and inactivated detoxification system genes, resulting in increased sensitization to ciprofloxacin. This study highlights the potential of targeting recA gene and oxidative detoxification stress systems to enhance antibiotic-induced bacterial cell death.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Kathryn J. Stratton, Matthew J. Bush, Govind Chandra, Clare E. M. Stevenson, Kim C. Findlay, Susan Schlimpert
Summary: DNA damage triggers the SOS response in bacteria, and the number of genes regulated by LexA varies between different organisms. This study demonstrates the importance of RecA and LexA for the survival and normal development of Streptomyces venezuelae during DNA-damaging conditions. Through transcriptional profiling and ChIP-seq analysis, the LexA regulon and core DNA damage repair genes were identified in response to treatment with mitomycin C.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biology
Magdalena Metzger, Ara Hacobian, Lisa Karner, Leonie Krausgruber, Johannes Grillari, Peter Dungel
Summary: The increase in antibiotic resistance poses a significant global challenge, and blue light therapy may offer an alternative treatment option. Research shows that blue light with a wavelength of 475 nm is only effective against certain bacterial strains lacking the RecA DNA repair mechanism. Inserting the recA gene reduces the sensitivity of responsive bacterial strains, but inhibiting RecA activity does not increase bactericidal action. Combining a multitarget therapy like blue light with a RecA targeting treatment could be a promising strategy to overcome antibiotic resistance in the future.
Article
Multidisciplinary Sciences
Mohammed A. Thabet, Jose R. Penades, Andreas F. Haag
Summary: Bacteriophages play a significant role in the dissemination of bacterial virulence, pathogenicity, and antimicrobial resistance. A study has discovered the unexpected involvement of the bacterial protease ClpX in the activation of bacteriophages.
NATURE COMMUNICATIONS
(2023)
Article
Infectious Diseases
S. Diaz-Diaz, Pilar Yerbes, E. Recacha, B. de Gregorio-Iaria, Marina R. Pulido, Maria Romero-Munoz, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: RecA inhibition is a potential strategy to combat antimicrobial resistance. This study investigates the impact of RecA inactivation on heteroresistance in Escherichia coli clinical isolates. The results show that deletion of the recA gene significantly reduces heteroresistant subpopulations, especially against quinolones or beta-lactams. The molecular basis of heteroresistance is associated with an increase in copy number of resistance genes, which is suppressed in the absence of the recA gene.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Biochemistry & Molecular Biology
Miquel Sanchez-Osuna, Pilar Cortes, Mark Lee, Aaron T. Smith, Jordi Barbe, Ivan Erill
Summary: Lesions to DNA can threaten cell survival by compromising chromosome integrity, and the bacterial SOS response is a common transcriptional regulatory mechanism to address DNA damage. A novel family of transcriptional repressor proteins in the Bacteroidetes has been identified, orchestrating the canonical response to DNA damage in this phylum. The discovery sheds light on the origin and plasticity of the transcriptional network involved in the SOS response.
NUCLEIC ACIDS RESEARCH
(2021)
Editorial Material
Microbiology
Leslie A. Gregg-Jolly
Summary: This article reveals the regulatory mechanism of the SOS response in Acinetobacter baumannii and provides new therapeutic targets for the development of antimicrobial therapies.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yongzhong Lu, Linyue Cheng
Summary: LexA-binding sites and LexA regulons were predicted in 23 Proteus genomes, showing a wide distribution of the SOS system in this genus; proteomic and RT-qPCR analyses revealed that under iron deficiency, the members of LexA regulon were regulated in different ways, suggesting a precise regulation mechanism of SOS response.
Article
Biology
Cody E. FitzGerald, James P. Keener
Summary: Mycobacteria possess a complex DNA damage repair system governed by two major DNA damage responses, the LexA/RecA-dependent response and the PafBC-mediated response. The PafBC-mediated response sensitizes the overall DNA damage response and lowers the DNA damage rate threshold for activation. The mathematical characterization of the interaction between the LexA/RecA-dependent response and the PafBC-mediated response is still pending.
JOURNAL OF THEORETICAL BIOLOGY
(2021)
Article
Biology
Aleksandr Alekseev, Georgii Pobegalov, Natalia Morozova, Alexey Vedyaykin, Galina Cherevatenko, Alexander Yakimov, Dmitry Baitin, Mikhail Khodorkovskii
Summary: This study investigated the regulation of RecA-ssDNA filaments by RecX using a single-molecule approach and found that RecX effectively binds the inactive conformation of RecA-ssDNA filaments and slows down the transition to the active state.
Review
Cell Biology
Laurence Blanchard, Arjan de Groot
Summary: Deinococcus bacteria are highly resistant to radiation and can repair their genome in an almost error-free manner, regulated by metallopeptidase IrrE and repressor DdrO. Deinococcus radiodurans, the most studied species, does not possess error-prone translesion DNA polymerases, which may be crucial for error-free repair.
Article
Biotechnology & Applied Microbiology
Duohong Sheng, Ye Wang, Zhiwei Jiang, Dongkai Liu, Yuezhong Li
Summary: The induction of ImuA in UV-C-irradiated Myxococcus xanthus occurred earlier than other genes, and its absence delayed bacterial regrowth and slightly reduced mutation frequency and UV resistance. ImuA inhibits the recombinase activity of RecA1, affecting SOS mutagenesis in M. xanthus.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Morteza Dehghan, Razieh Pourahmad-Jaktaji
TURKISH JOURNAL OF MEDICAL SCIENCES
(2015)
Article
Infectious Diseases
Razieh Pourahmad Jaktaji, Parvin Mohammadi
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2018)
Article
Microbiology
Razieh Pourahmad Jaktaji, Seyed Mohammad Lesani, Hoda Akhavan, Maryam Tanhaei
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
(2020)
Article
Microbiology
Razieh Pourahmad Jaktaji, Sayedeh Marzieh Nourbakhsh Rezaei
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
R. Pourahmad Jaktaji, S. Koochaki
Summary: This study investigated the antibacterial activity of honey, total alkaloids of Sophora alopecuroides (TASA) and matrine alone and in combination with antibiotics against multidrug-resistant Pseudomonas aeruginosa strains. The results showed a synergistic effect of matrine and TASA-honey in combination with ciprofloxacin against all tested isolates. These natural products can be used against overactivating MexAB-OprM but not MBL-producing P. aeruginosa isolates.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Razieh Pourahmad Jaktaji, Farzaneh Zargampoor
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2017)
Article
Medicine, General & Internal
Morteza Dehghan, Razieh Pourahmad-Jaktaji
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH
(2016)
Article
Pharmacology & Pharmacy
R. Pourahmad Jaktaji, F. Heidari
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2013)
Article
Medicine, Research & Experimental
Razieh Pourahmad Jaktaji, Nasim Jazayeri
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2013)
Article
Pharmacology & Pharmacy
Razieh Pourahmad Jaktaji, Rayhaneh Ebadi
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2013)
Article
Pharmacology & Pharmacy
Razieh Pourahmad Jaktaji, Rayhaneh Ebadi, Marzieh Karimi
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2012)
Article
Pharmacology & Pharmacy
Razieh Pourahmad Jaktaji, Ehsan Mohiti
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH
(2010)
Article
Biochemistry & Molecular Biology
BW Trautinger, RP Jaktaji, E Rusakova, RG Lloyd
Article
Biochemistry & Molecular Biology
RP Jaktaji, RG Lloyd
MOLECULAR MICROBIOLOGY
(2003)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.