期刊
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
卷 14, 期 2, 页码 -出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/cshperspect.a040683
关键词
-
类别
资金
- DFG Priority Program 2191 Molecular Mechanisms of Functional Phase Separation [RI1283/16-1]
Self-organizing membraneless chromatin subcompartments play a crucial role in eukaryotic cells by efficiently conducting genome activities and establishing specific cellular programs. However, the mechanisms for their formation are only partially understood, with recent studies suggesting liquid-liquid phase separation (LLPS) of proteins and RNAs. Additionally, the folding of chromatin can partition the genome into distinct domains. This article discusses the interplay between chromatin organization, chromatin binding, and LLPS by comparing three prototypical chromatin subcompartments.
In eukaryotic cells, protein and RNA factors involved in genome activities like transcription, RNA processing, DNA replication, and repair accumulate in self-organizing membraneless chromatin subcompartments. These structures contribute to efficiently conduct chromatin-mediated reactions and to establish specific cellular programs. However, the underlying mechanisms for their formation are only partly understood. Recent studies invoke liquid-liquid phase separation (LLPS) of proteins and RNAs in the establishment of chromatin activity patterns. At the same time, the folding of chromatin in the nucleus can drive genome partitioning into spatially distinct domains. Here, the interplay between chromatin organization, chromatin binding, and LLPS is discussed by comparing and contrasting three prototypical chromatin subcompartments: the nucleolus, clusters of active RNA polymerase II, and pericentric heterochromatin domains. It is discussed how the different ways of chromatin compartmentalization are linked to transcription regulation, the targeting of soluble factors to certain parts of the genome, and to disease-causing genetic aberrations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据