Removal of pentachlorophenol from contaminated wastewater using phytoremediation and bioaugmentation processes

The phytoremediation procedure was conducted by Lemna gibba (L) and Typha angustifolia (T) and the bioaugmentation one was operated by P.putida HM627618. The ability of the selected P.putida HM627618 to tolerate and remove PCP (200 mg L-1) was performed by HPLC analysis and the optical density at 600 nm measurement. Five different experiments were conducted in STWW for PCP testing removal (100 mg L-1): two phytoremediation essays (T + PCP; L + PCP), three bioaugmentation-phytoremediation essays (T + B + PCP; L + B + PCP; L + T + B + PCP) and a negative control assay with PCP. Some analytical parameters were determined in this study such as bacterial count, chlorophylls a and b, COD, pH and PCP content. The main found results showed that the average PCP removal by P.putida HM627618 was around 87.5% after 7 days of incubation, and 88% of PCP removal achieved by treatment (T + B) after 9 days. During these experiments, pH, COD and chloride content showed a net increase in all treatments. The chlorophylls a and b in case of (T) and (L) phytoremediation showed a decrease with a value less than 10 mu g/mg of fresh material after 20 days of cultivation. HIGHLIGHTS The use of macrophytes with two categories: aquatic fixed and Floating model to treat polychlorinated pollutants from wastewater. This study evaluate the efficiency of Lemna Gibba and typha angustifolia to remove pentachlorophenol from aqueous solution, further, and combined process of phytoremediation and bioaugmentaion efficacy in PCP removal by the plant.

Automated summary

The study evaluated the efficiency of Lemna Gibba and typha angustifolia in removing pentachlorophenol from aqueous solution, as well as the combined process of phytoremediation and bioaugmentation in PCP removal by the plant. Results showed that P.putida HM627618 and T + B treatment had good removal efficiency for PCP within a certain period, but chlorophyll a and b content decreased during the phytoremediation process.