Article
Chemistry, Analytical
Dan Zhao, Jiutang Tang, Qin Tan, Xiaohong Xie, Xin Zhao, Dingpei Xing
Summary: In this study, a dual-amplified biosensing method based on CRISPR/Cas13a-triggered Cas12a, namely Cas13a-12a amplification, was developed for miRNA-155 detection. The detection limit of this amplification strategy was as low as 0.35 fM, which was much lower than that of the only Cas13a-based assay. Accurate quantification of miRNA-155 expression levels in different cancer patients was achieved using this strategy. Hence, the developed method provides a supersensitive and highly specific miRNA sensing platform for clinical applications.
Article
Chemistry, Analytical
Zefeng Mao, Xiaojuan Wang, Ruipeng Chen, Zixuan Zhou, Shuyue Ren, Jun Liang, Zhixian Gao
Summary: The CRISPR-Cas system has made significant breakthroughs in the field of molecular diagnostics. In this study, a biosensing method powered by CRISPR/Cas12a was designed to detect ochratoxin A. Compared to traditional fluorescent probes, this method is faster and has comparable detection capabilities.
Article
Food Science & Technology
Zefeng Mao, Ruipeng Chen, Xiaojuan Wang, Zixuan Zhou, Yuan Peng, Shuang Li, Dianpeng Han, Sen Li, Yu Wang, Tie Han, Jun Liang, Shuyue Ren, Zhixian Gao
Summary: The use of molecular detection in food safety has become urgent due to the detection of novel coronavirus events in the food cold chain. This review introduces a new molecular diagnostic technology based on CRISPR-Cas12a and summarizes its applications in various aspects of food safety. The biosensor analytical technology has great potential in qualitative and quantitative analysis of infectious pathogens.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Shaohua Gong, Xi Wang, Ping Zhou, Wei Pan, Na Li, Bo Tang
Summary: In this study, a CRISPRCas12a-based biosensing platform was developed for sensitive colorimetric detection of dual miRNAs. The method allows direct differentiation by the naked eye and detection of miRNA in real human serum, providing potential for precise disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaomei Mu, Xin Wang, Yuxin Qin, Yong Huang, Jianniao Tian, Shulin Zhao
Summary: We developed a biosensing platform using CRISPR/Cas system and a fluorescent label-free biosensor for sensitive and specific detection of disease-related biomarkers. The system utilizes an exonuclease III-mediated target cycle and a universal trigger chain to amplify the signal. The platform can be easily transformed to detect proteins, small molecules, or microRNA in biological samples.
Article
Microbiology
Tian Luan, Lu Wang, Jiyu Zhao, Hui Luan, Yueling Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Wanjiang Zhang, Gang Li
Summary: In this study, a rapid detection platform called Card was developed for Actinobacillus pleuropneumoniae, which shows high sensitivity and specificity. The detection process is fast, and the results are consistent with traditional PCR methods. Card has potential applications in both diagnostic laboratories and field settings.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Shuang Liu, Siyuan Huang, Fang Li, Yuanyuan Sun, Jin Fu, Fei Xiao, Nan Jia, Xiaolan Huang, Chunrong Sun, Juan Zhou, Yi Wang, Dong Qu
Summary: The study developed a simple, rapid, sensitive, and specific detection platform for P. aeruginosa infection. The newly developed P. aeruginosa-CRISPR-RPA assay showed high sensitivity and specificity, and could complete P. aeruginosa detection within half an hour.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Dong-Xia Wang, Ya-Xin Wang, Jing Wang, Jia-Yi Ma, Bo Liu, An-Na Tang, De-Ming Kong
Summary: This study demonstrates that MnO2 nanosheets are an excellent carrier for the CRISPR/Cas12a system, enabling efficient delivery and rapid release in living cells. The use of MnO2 nanosheets improves the performance of live-cell biosensing analysis compared to commercial carriers.
Article
Chemistry, Multidisciplinary
Yunping Wu, Dingran Chang, Yangyang Chang, Qiang Zhang, Yi Liu, John D. Brennan, Yingfu Li, Meng Liu
Summary: CRISPR-Cas systems are widely used in biosensor development, but translating recognition events into measurable signals remains a challenge. This study demonstrates that circular CRISPR RNAs (crRNAs) render Cas12a incapable of DNA cutting and trans cleavage. Linearization of circular crRNAs using NAzymes activates CRISPR-Cas12a functions, allowing versatile biosensing.
Article
Engineering, Biomedical
Kathrine Curtin, Jing Wang, Bethany J. Fike, Brandi Binkley, Peng Li
Summary: This article reports a 3D-printed composable microfluidic plate (cPlate) device for multiplexed CRISPR-Cas12a assay. The device combines loop-mediated isothermal amplification (LAMP) and CRISPR-Cas12a readout, enabling a simple and high-throughput workflow with low reagent consumption. The optimized device achieves sensitive detection of four waterborne pathogens within 1 hour, making it suitable for low-resource settings.
BIOMEDICAL MICRODEVICES
(2023)
Review
Chemistry, Multidisciplinary
Yuyan Shi, Xiaoyi Fu, Yao Yin, Fangqi Peng, Xia Yin, Guoliang Ke, Xiaobing Zhang
Summary: CRISPR-Cas12a is a promising gene editing technology that utilizes an RNA-guided nuclease. It excels not only in gene editing but also shows great potential in the field of biosensing.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Plant Sciences
Qiang Wang, Ming Qin, Jeffrey J. Coleman, Wenjing Shang, Xiaoping Hu
Summary: Verticillium wilt, caused by Verticillium dahliae, is a significant soilborne plant disease worldwide. In this study, a detection method using CRISPR/Cas12a and RPA technologies was developed for accurate and rapid detection of V. dahliae. The detection system had a lower detection limit of approximately 10 copies of genomic DNA per reaction and could detect as little as one microsclerotium per gram of soil. This study expands the application of CRISPR/Cas12a nucleic acid detection to soilborne crop diseases and contributes to the development of field-deployable diagnostic tools.
Article
Chemistry, Analytical
Peipei Qin, Pinru Chen, Nan Deng, Liu Tan, Bin-Cheng Yin, Bang-Ce Ye
Summary: This study proposes a method to directly regulate the trans cleavage activity of Cas12a using an allosteric DNA probe, enabling the sensing of different types of analytes. The proposed strategy utilizes an allosteric inhibitor probe that can induce a change in conformation and remove its inhibitory function on Cas12a upon binding to a specific target. The method was validated for detecting adenosine triphosphate and let-7a, demonstrating excellent specificity.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xiangxiang Zhao, Shanshan Li, Guang Liu, Zhong Wang, Zhiheng Yang, Quanwei Zhang, Mindong Liang, Jiakun Liu, Zilong Li, Yaojun Tong, Guoliang Zhu, Xinye Wang, Lan Jiang, Weishan Wang, Gao-Yi Tan, Lixin Zhang
Summary: This study introduces a versatile biosensing platform that combines CRISPR-Cas with aptamers for detecting proteins and small molecules in human serum. The platform offers advantages of simplicity, sensitivity, short detection time, and low cost, showing great potential in diverse analyte detection.
Article
Chemistry, Analytical
Qing-Nan Li, Dong-Xia Wang, Gui-Mei Han, Bo Liu, An-Na Tang, De-Ming Kong
Summary: The background issue in CRISPR/Cas12a-based biosensors has been efficiently solved by designing the activator strand (AS) using the RESET effect. The low background allows for sensitive detection and accurate imaging analysis of different targets in live cells, and the high-performance biosensors have potential for point-of-care testing. Several biosensing systems have been developed for the detection of nucleic acid, small molecules, and enzymes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fei Deng, Yi Li, Laicong Qiao, Ewa Goldys
Summary: A CRISPR/Cas12a assisted on-fibre immunosensor (CAFI) was developed to track trace amounts of analytes directly from low volumes of complex biological samples. CAFI showed a 1,000-fold higher sensitivity compared to conventional methods, with a tunable linear detection range that meets the requirements of demanding diagnostic scenarios. The CAFI biosensing system was successfully demonstrated for the detection of IFN-gamma and other analytes.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Yingying Li, Yang Hu, Tao Chen, Yan Chen, Yi Li, Haibo Zhou, Danting Yang
Summary: This review discusses and summarizes advanced detection and sensing strategies for P. aeruginosa cells and its associated quorum sensing molecules over the past ten years. The aim is to achieve rapid, sensitive, and cost-effective diagnosis for infection. The principles of commonly used sensing strategies are briefly introduced, followed by the advancement of these techniques in detecting P. aeruginosa cells and quorum sensing biomarkers. Additionally, the integration of novel platforms for clinical application is highlighted. The current achievements are summarized, and challenges and prospects for the future are proposed.
Article
Chemistry, Analytical
Yi Li, Fei Deng, Ewa M. Goldys
Summary: The study demonstrates the successful integration of CRISPR/Cas12a-based signal amplification into immunoassays using a functional hybrid conjugate of antibody and DNA oligonucleotide. This approach improves the sensitivity of conventional immunoassays, allowing for the detection of small proteins with attomolar sensitivity. The CRISPR-based Universal Immunoassay Signal Enhancer (CRUISE) can be applied to various immunoassays and has the potential to enhance sensitivity and expand the detection range.
Article
Medicine, Research & Experimental
Rui Sang, Fei Deng, Alexander Engel, Ewa Goldys, Wei Deng
Summary: This study combines X-ray induced photodynamic therapy (X-PDT) and chemo-drug to effectively treat colorectal cancer cells. The developed FA-LPNPs-VP-5-FU nanoparticles generate reactive oxygen species upon exposure to X-ray radiation, inhibiting cancer cell growth and proliferation through the apoptosis and necrosis pathway. The combined treatment shows better antitumour effect than each modality alone.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Multidisciplinary
Chengchen Zhang, Jianbo Tang, Wanjie Xie, Francois-Marie Allioux, Zhenbang Cao, Joanna M. Biazik, Mohammad Tajik, Fei Deng, Yi Li, Roozbeh Abbasi, Mahroo Baharfar, Maedehsadat Mousavi, Dorna Esrafilzadeh, Kourosh Kalantar-Zadeh
Summary: This study investigates the impact of inorganic particle density on the migratory behaviors of macrophages. The results demonstrate that higher particle density leads to decreased mobility of macrophages. This finding serves as a reminder to consider particle density in the design of biological applications.
Article
Chemistry, Analytical
Fei Deng, Yi Li, Tim Hall, Graham Vesey, Ewa M. Goldys
Summary: Protein conjugates are widely used in biochemistry, particularly in diagnostic platforms like antibody-based immunoassays. In this study, antibody was directly conjugated with Cas12a/gRNA ribonucleoprotein, resulting in a conjugate with desired functions for immunoassays and signal amplification. The approach successfully detected different types of targets, with high sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang
Summary: A novel approach called the CRISPR/Cas12a enzyme-linked aptamer-sorbent assay (CLASA) was developed for ultrasensitive detection of Ochratoxin A (OTA). This method not only overcomes the limitations of traditional methods, but also has the flexibility to detect other small molecular targets. It holds great promise for applications in various fields requiring sensitive and specific detection of diverse analytes.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Fei Deng, Laicong Qiao, Yi Li
Summary: Cytokines play a crucial role in the immune response. This study presents an optical fibre based biosensor for multiplex detection of cytokines. The biosensor showed good performance in measuring proinflammatory cytokines and could be applied for the detection of other proteins. The fibre based multiplex biosensor is important for studying inflammatory processes and disease monitoring.
SENSING AND BIO-SENSING RESEARCH
(2022)
Article
Biochemical Research Methods
Lin Ding, Sajad Razavi Bazaz, Timothy Hall, Graham Vesey, Majid Ebrahimi Warkiani
Summary: This study presents a method to purify Giardia from fecal samples using microfluidics. The proposed rigid spiral microfluidic device effectively separates Giardia from debris, with high recovery rates and fast purification. This device is highly scalable and versatile, with the potential to purify other gastrointestinal pathogens of similar size.
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.
