期刊
ULTRASONICS SONOCHEMISTRY
卷 79, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ultsonch.2021.105764
关键词
High-intensity ultrasound; Acoustic pressure; Simulation; Staphylococcus aureus; Biofilm control; Stimulation
资金
- National Nature Science Foundation of China [32001627, 32172326]
- National Key R&D Program of China [2018YFC1602300, 2020YFC1606800]
- Key R&D Program of Jiangsu Province [BE2019362]
- Fundamental Research Funds for the Central Universities [JUSRP11904, JUSRP321014]
- Science and Technology Project of Market Supervision Administration of Jiangsu Province [KJ204132]
- National Training Program of Innovation and Entrepreneurship for Undergraduates [2021020Z, 202010295047Z]
- National first-class discipline program of Food Science and Technology [JUFSTR20180509]
- High-level Innovation and Entrepreneurship Talents Introduction Program of Jiangsu Province (Su Talent Office [2019]) [20]
This study demonstrates that high-intensity ultrasound can effectively control or stimulate Staphylococcus aureus biofilm. Under appropriate power, HIU can efficiently clear biofilms, while low-power treatments may promote biofilm formation.
This study evaluated efficacy of high-intensity ultrasound (HIU) on controlling or stimulating Staphylococcus aureus biofilm. Acoustic pressure distribution on the surface of glass slide cultivated S. aureus biofilm was first simulated as a standardized parameter to reflect sono-effect. When the power of HIU was 240 W with acoustic pressure of -1.38x10(5) Pa, a reasonably high clearance rate of S. aureus biofilm was achieved (96.02%). As an all-or-nothing technique, the HIU did not cause sublethal or injury of S. aureus but inactivate the cell directly. A further evaluation of HIU-induced stimulation of biofilm was conducted at a low power level (i.e. 60 W with acoustic pressure of -6.91x10(4) Pa). The low-power-long-duration HIU treatment promoted the formation of S. aureus biofilm and enhanced its resistance as proved by transcriptional changes of genes in S. aureus, including up-regulations of rbf, sigB, lrgA, icaA, icaD, and down-regulation of icaR. These results indicate that the choose of input power is determined during the HIU-based cleaning and processing. Otherwise, the growth of S. aureus and biofilm formation are stimulated when treats by an insufficiently high power of HIU.
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