期刊
SCIENCE
卷 373, 期 6550, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abh0556
关键词
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资金
- European Research Council Starting Grant (SexMeth) [804981]
- Sainsbury Charitable Foundation
- Biotechnology and Biological Sciences Research Council (BBSRC) [BBS0096201, BBP0135111]
- BBSRC David Phillips Fellowship [BBL0250431]
- European Research Council (ERC) [804981] Funding Source: European Research Council (ERC)
The study reveals the molecular mechanism of DNA methylation reprogramming in plant male germline. Small interfering RNAs (siRNAs) synthesized by meiocyte nurse cells establish genic methylation and safeguard genome integrity through tapetal siRNAs.
The plant male germline undergoes DNA methylation reprogramming, which methylates genes de novo and thereby alters gene expression and regulates meiosis. Here, we reveal the molecular mechanism underlying this reprogramming. We demonstrate that genic methylation in the male germline, from meiocytes to sperm, is established by 24-nucleotide small interfering RNAs (siRNAs) transcribed from transposons with imperfect sequence homology. These siRNAs are synthesized by meiocyte nurse cells (tapetum) through activity of CLSY3, a chromatin remodeler absent in other anther cells. Tapetal siRNAs govern germline methylation throughout the genome, including the inherited methylation patterns in sperm. Tapetum-derived siRNAs also silence germline transposons, safeguarding genome integrity. Our results reveal that tapetal siRNAs are sufficient to reconstitute germline methylation patterns and drive functional methylation reprogramming throughout the male germline.
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