期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2103730118
关键词
aging; PD-1; immunotherapy; T cell subset; one-carbon metabolism
资金
- Japan Agency for Medical Research and Development [20cm0106302h0005]
- Japan Society for the Promotion of Science KAKENHI [20K07615]
- Grants-in-Aid for Scientific Research [20K07615] Funding Source: KAKEN
CD8(+) T cells play a crucial role in antitumor immune responses, but this function is suppressed in aged individuals. The mechanism of this age-related immune decline is unclear. This study found that inefficient generation of a specific CD8(+) T cell subset may contribute to age-related resistance to PD-1 blockade, which can be overcome by strong TCR stimulation.
CD8(+) T cells play a central role in antitumor immune responses that kill cancer cells directly. In aged individuals, CD8(+) T cell immunity is strongly suppressed, which is associated with cancer and other age related diseases. The mechanism underlying this age-related decrease in immune function remains largely unknown. This study investigated the role of T cell function in age-related unresponsiveness to PD-1 blockade cancer therapy. We found inefficient generation of CD44(low)CD62L(low) CD8(+) T cell subset (P4) in draining lymph nodes of tumor-bearing aged mice. In vitro stimulation of naive CD8(+) T cells first generated P4 cells, followed by effector/memory T cells. The P4 cells contained a unique set of genes related to enzymes involved in one-carbon (1C) metabolism, which is critical to antigen-specific T cell activation and mitochondrial function. Consistent with this finding, 1C-metabolism-related gene expression and mitochondrial respiration were down-regulated in aged CD8(+) T cells compared with young CD8(+) T cells. In aged OVA-specific T cell receptor (TCR) trans genic mice, ZAP-70 was not activated, even after inoculation with OVA-expressing tumor cells. The attenuation of TCR signaling appeared to be due to elevated expression of CD45RB phosphatase in aged CD8(+) T cells. Surprisingly, strong stimulation by nonself cell injection into aged PD-1-deficient mice restored normal levels of CD45RB and ameliorated the emergence of P4 cells and 1C metabolic enzyme expression in CD8(+) T cells, and antitumor activity. These findings indicate that impaired induction of the P4 subset may be responsible for the age-related resistance to PD-1 blockade, which can be rescued by strong TCR stimulation.
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