Article
Immunology
Rui Zhou, Jingjing Lu, Jun Wang, Bingbing Xiao
Summary: Seven Gardnerella genomospecies were detected in Chinese BV patients, but no association between their distribution and BV occurrence or prognosis was found. The relative abundance of L. iners was higher in cured patients, and its antimicrobial activity against G. vaginalis and F. vaginae was validated.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tongyang Deng, Anquan Shang, Ying Zheng, Lianzhen Zhang, Hong Sun, Wei Wang
Summary: The aim of this study was to explore a new marker for the quantitative detection of bacterial vaginosis (BV) with high sensitivity and specificity. By comparing the differences in bacterial abundance between BV, healthy, and BV-intermediate groups using 16S rRNA-sequencing, candidate markers were identified. Real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was then used to quantitatively detect these candidate markers and evaluate their sensitivity and specificity. Four new indicators for BV detection were discovered, with log L. crispatus/G. vaginalis (L/G) < 0 being the best indicator. A molecular diagnostic system independent of the Nugent Score could have an important impact on the clinical management of BV.
Article
Immunology
Yuanhui He, Risu Na, Xiaoxi Niu, Bingbing Xiao, Huixia Yang
Summary: This study investigated the impact of Lactobacillus rhamnosus and Lactobacillus casei on Gardnerella species biofilm formation, finding that adding L. rhamnosus at different stages effectively suppressed biofilm formation. Additionally, the quantity of biofilm was found to be highest at a pH of 5.5 and after 36 hours.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Carolina Sanita Tafner Ferreira, Camila Marconi, Cristina M. G. L. Parada, Jacques Ravel, Marcia Guimaraes da Silva
Summary: Sialidase activity in cervicovaginal fluid is associated with changes in bacterial composition, potentially leading to diminished local defenses against pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Jacob H. Elnaggar, Caleb M. Ardizzone, Nuno Cerca, Evelyn Toh, Pawel Laniewski, Rebecca A. Lillis, Melissa M. Herbst-Kralovetz, Alison J. Quayle, Christina A. Muzny, Christopher M. Taylor
Summary: Bacterial vaginosis (BV) is the most common vaginal dysbiosis characterized by the formation of a polymicrobial biofilm on vaginal epithelial cells. The standard method for quantifying the bacterial burden of BV biofilm using Escherichia coli as a reference has limitations. A novel qPCR standard using different combinations of vaginal bacteria was proposed to accurately quantify bacterial burden in vaginal microbial communities, which showed superior accuracy compared to the traditional E. coli reference standard.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Agnieszka Latka, Leen Van Simaey, Marijke Reynders, Piet Cools, Tess Rogier, Barbara Lebbe, Lorenzo Corsini, Christine Landlinger, Mario Vaneechoutte
Summary: The quantification of living cells in biofilm or after eradication treatments is problematic. This study assessed the performance of pre-treatment with propidium monoazide (PMAxx) to prevent qPCR-based amplification of DNA from killed cells. The optimized PMAxx treatment condition showed a significant reduction of Gardnerella cells, validating its use in vaginal samples from women with bacterial vaginosis.
Article
Microbiology
Qiuxiang Zhang, Qiuhan Cheng, Shumao Cui, Jianxin Zhao, Wei Chen, Hao Zhang
Summary: This study evaluated the inhibitory effect of Lactobacillus gasseri CCFM1201 on Gardnerella vaginalis, the core pathogen of bacterial vaginosis. In vitro and in vivo experiments showed that L. gasseri CCFM1201 can inhibit the formation of G. vaginalis biofilm and its adhesion capacity. Furthermore, L. gasseri CCFM1201 reduced G. vaginalis in mice, modulated the inflammatory response, and improved histopathological damage to vaginal tissue.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Immunology
Xiaodi Chen, Yune Lu, Tao Chen, Rongguo Li
Summary: The vaginal microbiome is crucial for women's health, with bacterial vaginosis (BV) associated with various adverse gynecologic and obstetric outcomes. Research on diagnosis and treatment strategies for BV is making progress.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Sunwha Park, Young-Ah You, Young-Han Kim, Eunjin Kwon, AbuZar Ansari, Soo Min Kim, Gain Lee, Young Min Hur, Yun Ji Jung, Kwangmin Kim, Young Ju Kim
Summary: Ureaplasma and Prevotella colonization is associated with preterm birth, but not all colonizations cause it. This study found a positive correlation between Ureaplasma and other genera with preterm birth, and a negative correlation between Lactobacillus and term birth, similar to Prevotella. Balanced colonization between Lactobacillus and Ureaplasma and Prevotella is important to prevent preterm birth.
SCIENTIFIC REPORTS
(2022)
Review
Infectious Diseases
Rebecca Jane Joseph, Hooi-Leng Ser, Yi-He Kuai, Loh Teng-Hern Tan, Valliammai Jayanthi Thirunavuk Arasoo, Vengadesh Letchumanan, Lijing Wang, Priyia Pusparajah, Bey-Hing Goh, Nurul-Syakima Ab Mutalib, Kok-Gan Chan, Learn-Han Lee
Summary: Bacterial vaginosis, caused by an imbalance in the vaginal microbiota, affects one-third of women worldwide and is associated with high treatment failure and recurrence rates. Current research is focused on alternative treatments like probiotics and vaginal microbiota transplants to improve treatment outcomes and reduce colonization by pathogenic microbes.
Article
Biotechnology & Applied Microbiology
Yu-Ping Huang, Jie-Yan Shi, Si-Chen Luo, Shao-Yan Xu, Jia-Dong Zhang, Istvan Molnar, Qiong-Qiong Yang, Bo-Bo Zhang
Summary: This study found that acetic acid and lactic acid in the cell-free supernatant of L. rhamnosus inhibit the growth of G. vaginalis by altering the morphology of the bacteria and affecting their ATP synthesis. The results provide valuable information for the use of L. rhamnosus in the treatment of bacterial vaginosis.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Christine Landlinger, Vera Oberbauer, Lenka Podpera Tisakova, Timo Schwebs, Rocio Berdaguer, Leen Van Simaey, Mario Vaneechoutte, Lorenzo Corsini
Summary: This study investigates the potential mechanisms of therapy failure in bacterial vaginosis treatment, revealing that metronidazole and clindamycin face issues of resistance formation and ineffectiveness on biofilm. However, a novel endolysin PM-477 shows high effectiveness in eliminating Gardnerella and has high resilience to resistance formation, making it a promising alternative for the treatment of bacterial vaginosis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Veterinary Sciences
Jinok Kwak, Sriniwas Pandey, Jinho Cho, Minho Song, Eun Sol Kim, Hyunok Doo, Gi Beom Keum, Sumin Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Jeongyun Kim, Hyeun Bum Kim
Summary: This study developed a mouse model for human BV induced by G. vaginalis, and demonstrated comparable clinical attributes to patients with BV.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Hanyu Qin, Yun Liu, Zhengyuan Zhai, Bingbing Xiao
Summary: This study found that different subgroups of Gardnerella exhibit differences in biofilm formation ability and antibiotic resistance. Strains with strong biofilm ability contain more genes involved in carbohydrate synthesis and metabolism. These findings contribute to the development of novel treatments for bacterial vaginosis.
Article
Infectious Diseases
Abebaw Bitew, Abeba Mengist, Habtamu Belew, Alemayehu Reta, Felege Hiwot
Summary: The study conducted at Felege Hiwot Referral Hospital found a prevalence rate of 39.5% for bacterial vaginosis among women of reproductive age, with S. aureus, G. vaginalis, S. agalactiae, and E. coli being the predominant bacteria. The research also highlighted antibiotic resistance in certain bacteria causing bacterial vaginosis.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Jack D. Sobel, Navkiranjot Kaur, Nicole A. Woznicki, Dina Boikov, Tina Aguin, Gurveer Gill, Robert A. Akins
INFECTION AND DRUG RESISTANCE
(2019)
Article
Multidisciplinary Sciences
Ashomathi Mollin, Mounika Katta, Jack D. Sobel, Robert A. Akins
Summary: Bacterial vaginosis (BV) is associated with vaginal dysbiosis and high recurrence rates. This study found that different bacterial species and their abundance are correlated with treatment outcomes, suggesting potential prognostic and therapeutic significance.