期刊
PLANT AND CELL PHYSIOLOGY
卷 63, 期 1, 页码 30-44出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcab137
关键词
Ammonium; Nitrate; Nitrogen deficiency; Populus; Root zone; Uptake
资金
- Fundamental Research Funds for the Central Non-profit Research Institutions of CAF [CAFYBB2019SY013]
- National Key Research and Development Program [2016YFD0600105]
This study investigated the physiological and transcriptomic regulation mechanisms underlying the distinct net fluxes of NH4+ and NO3- in different root segments of Populus species under low nitrogen conditions. The results suggest that the hub gene coexpression networks play pivotal roles in the regulation of N uptake and assimilation, amino acid metabolism, and the levels of organic acids in poplars' root segments in response to low N availability.
To investigate physiological and transcriptomic regulation mechanisms underlying the distinct net fluxes of NH4+ and NO3- in different root segments of Populus species under low nitrogen (N) conditions, we used saplings of Populus x canescens supplied with either 500 (normal N) or 50 (low N) mu M NH4NO3. The net fluxes of NH4+ and NO3-, the concentrations of NH4+, amino acids and organic acids and the enzymatic activities of nitrite reductase (NiR) and glutamine synthetase (GS) in root segment II (SII, 35-70 mm to the apex) were lower than those in root segment I (SI, 0-35 mm to the apex). The net NH4+ influxes and the concentrations of organic acids were elevated, whereas the concentrations of NH4+ and NO3- and the activities of NiR and GS were reduced in SI and SII in response to low N. A number of genes were significantly differentially expressed in SII vs SI and in both segments grown under low vs normal N conditions, and these genes were mainly involved in the transport of NH4+ and NO3-, N metabolism and adenosine triphosphate synthesis. Moreover, the hub gene coexpression networks were dissected and correlated with N physiological processes in SI and SII under normal and low N conditions. These results suggest that the hub gene coexpression networks play pivotal roles in regulating N uptake and assimilation, amino acid metabolism and the levels of organic acids from the tricarboxylic acid cycle in the two root segments of poplars in acclimation to low N availability.
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