Article
Agricultural Engineering
Marcos Vinicius Silva de Andrade, Renato Delmondez de Castro, Diego da Silva Cunha, Valdir Gomes Neto, Maria Gabriela Aparecida Carosio, Antonio Gilberto Ferreira, Lourdes Cardoso de Souza-Neta, Luzimar Gonzaga Fernandez, Paulo Roberto Ribeiro
Summary: Research demonstrates that Stevia rebaudiana's response to light conditions affects the accumulation of antioxidant-like metabolites in its leaves, possibly linked to total phenolic content. Variations in SOD activity among plants under different photoperiods suggest a synergistic interaction between SOD isoforms and phenolic compounds to mitigate oxidative stress caused by light exposure.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Agricultural Engineering
Djessica Tatiane Raspe, Simone Rocha Ciotta, Maria Rosa Trentin Zorzenon, Antonio Sergio Dacome, Camila da Silva, Paula Gimenez Milani, Silvio Claudio da Costa
Summary: This study investigated ultrasound-assisted extraction of Stevia rebaudiana leaf compounds previously treated with ethanol. Results showed that applying maximum power, increasing solvent to leaf ratio, and raising ethanol percentage in the solvent contributed to higher yields and more sweeteners.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Muhammad Arslan Ahmad, Sadaf Chaudhary, Xu Deng, Mumtaz Cheema, Rabia Javed
Summary: Nanotechnology offers efficient options for nano-bio interface, and nanomaterials have emerged as strong elicitors in medicinal plants. Stevia rebaudiana, a well-known bio-sweetener, contains steviol glycosides (SGs) and other bioactive compounds with medicinal importance. Various in vitro and cell culture strategies are being adopted to enhance the production of SGs and increase the market value of stevia. Nanoparticles act as elicitors in stevia agronanotechnology, specifically triggering the production of rebaudioside A.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Shahnawaz, Devendra Kumar Pandey, Merinashwari Konjengbam, Padmanabh Dwivedi, Prabhjot Kaur, Vijay Kumar, Durga Ray, Puja Ray, Romaan Nazir, Harmeet Kaur, Sidharth Parida, Abhijit Dey
Summary: Plant cell and tissue culture offers a sustainable approach for producing secondary metabolites, while advancements in gene editing provide new possibilities for improving the quality and quantity of phytochemicals. Large-scale cultivation of Stevia rebaudiana enhances the yield of its low-calorie sweetener glycosides, making it a natural choice for diabetic and weight control products.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Sun Yu-ming, Huang Xiao-lei, Zhang Ting, Yang Yong-heng, Cheng Xiao-fang, Xu Xiao-yang, Yuan Hai-yan
Summary: The study found that potassium deficiency significantly reduces steviol glycosides content in stevia leaves, inhibits the expression of genes related to steviol glycosides synthesis, and affects the activity of sugar metabolism-related enzymes. There is a significant positive correlation between sucrose, glucose, and steviol glycosides content.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Plant Sciences
Hojjat Baroni-nezhad, Mojtaba Karimi, Hamidreza Motaghian, Fatemeh Direkvand-moghadam
Summary: Micronutrients like Copper, Iron, and Zinc do not significantly affect the growth of Stevia, but can markedly increase the content of sweet compounds such as steviol glycosides in the leaves.
JOURNAL OF PLANT NUTRITION
(2021)
Article
Food Science & Technology
Dj'essica Tatiane Raspe, Camila da Silva, Silvio Claudio da Costa
Summary: This review analyzes and discusses emerging techniques for extracting natural sweeteners and active compounds from Stevia rebaudiana Bertoni, demonstrating their impacts on extraction and comparing them with conventional techniques. These new technologies offer the advantages of simplicity, reduced energy and solvent usage, making them potentially suitable for industrial applications, but they still face limitations.
Article
Horticulture
Monika Tuleja, Michal Santocki, Michal Dziurka, Krystyna Musial, Ewa Capecka, Marta Libik-Konieczny
Summary: Seed germination in Stevia rebaudiana is poor, limiting generative reproduction. The study suggests that improper levels of gibberellins and redirection of metabolic pathways for secondary metabolites biosynthesis may cause difficulties in pollen tube germination, affecting seed development in the plant.
SCIENTIA HORTICULTURAE
(2021)
Article
Biotechnology & Applied Microbiology
Rizka Tamania Saptari, Rizkita Rachmi Esyanti, Riza Arief Putranto
Summary: This study demonstrates that inhibiting the late-stage GA biosynthesis pathway can increase the content of SG in stevia. The SG and GA pathways are related, as they share the same precursor. Inhibiting GA biosynthesis activates the SG biosynthesis pathway, leading to an increase in SG content.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Chemistry, Applied
Yaxian Liu, Xiao Hua, Menglei Zhang, Aibin Zhou, Xiangyun Zhou, Ruijin Yang
Summary: Mother liquor sugar (MLS) containing steviol glycosides (SGs) was crystallized and purified through reversed-phase chromatography, achieving a total recovery rate of 97.23% under optimal conditions. The two-step purification method is easy to operate and suitable for industrial scale-up.
Review
Food Science & Technology
Isabella Paola Schiatti Siso, Somaris E. Quintana, Luis Alberto Garcia Zapateiro
Summary: Stevia, a substitute for sucrose, low caloric, and natural sweetener, has various effects on the physicochemical, nutritional and technological properties of food products. Its application in different food categories can alter sensory characteristics and affect the properties of the final product.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Agricultural Engineering
Saroj Laha, Tsama Subrahmanyeswari, Sandeep Kumar Verma, Suchita N. Kamble, Sudhir Singh, Somnath Bhattacharyya, Saikat Gantait
Summary: In this study, a simple and eco-friendly method for synthesizing silver nanoparticles (AgNPs) using leaf extract of in vitro banana plantlets was established. The synthesized AgNPs were characterized and then used to study their effects on the growth and rebaudioside-A content of stevia under in vitro conditions. The results showed that 400 μM AgNPs concentration in 'semi-solid followed by liquid' medium exhibited the best growth response and highest rebaudioside-A production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Morteza Sheikhalipour, Gholamreza Gohari, Behrooz Esmaielpour, Sima Panahirad, Maryam Haghmadad Milani, Muhittin Kulak, Tibor Janda
Summary: Drought stress is a major constraint on crop growth and productivity. This study investigated the effects of melatonin and nano-titanium dioxide nanoparticles (TiO2 NPs) on the agronomic, physiological, and biochemical traits of stevia under moderate and severe drought stress. The results showed that the application of melatonin and TiO2 NPs improved plant traits, increased antioxidant activities, proline content, and secondary metabolites, and decreased malondialdehyde levels. The best treatment for alleviating the negative effects of drought stress and increasing secondary metabolites in stevia was foliar application of 100 μM melatonin and 10 mg/L TiO2 NPs.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agronomy
Asish Kumar Ghose, Siti Nor Akmar Abdullah, Muhammad Asyraf Md Hatta, Puteri Edaroyati Megat Wahab
Summary: A plant tissue culture protocol from stevia was optimized for the production of planting materials and rebaudioside A, a natural sweetener. By adjusting the culture medium and supplement concentrations, the survival rate and callus production were improved, resulting in higher yields of shoots and roots. The highest concentration of rebaudioside A was obtained using specific media conditions and correlated with the expression level of a biosynthetic gene.
Article
Agricultural Engineering
Naveed Ahmad, Abdur Rab, Muhammad Sajid, Nisar Ahmad, Hina Fazal, Mohammad Ali, Ulrika Egertsdotter
Summary: The study showed that differential sucrose feeding significantly affects the production and accumulation of SGs in Stevia SRC, with biomass accumulation and biosynthesis of major SGs being influenced by sucrose concentration. Adventitious root cultures could be a viable option for large-scale production of SGs, with the potential for enhancing biosynthesis of major SGs through sucrose regulation.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Mohamad Shazeli Che Zain, Soo Yee Lee, Murni Nazira Sarian, Sharida Fakurazi, Khozirah Shaari
Article
Chemistry, Medicinal
Hamza Ahmed Pantami, Muhammad Safwan Ahamad Bustamam, Soo Yee Lee, Intan Safinar Ismail, Siti Munirah Mohd Faudzi, Masatoshi Nakakuni, Khozirah Shaari
Article
Biochemistry & Molecular Biology
Mohamad Shazeli Che Zain, Soo Yee Lee, Nadiah Mad Nasir, Sharida Fakurazi, Khozirah Shaari
Article
Biochemistry & Molecular Biology
Mohamad Shazeli Che Zain, Muhamad Faris Osman, Soo Yee Lee, Khozirah Shaari
Summary: An improved and rapid analytical method, UHPLC-UV/PDA, was developed and validated for the quantification of luteolin and apigenin derivatives in oil palm leaf extracts. The method demonstrated good specificity, linearity, accuracy, precision, and robustness, with values within acceptable limits, and could potentially be used in standardized herbal preparations.
Article
Chemistry, Multidisciplinary
Anis Irfan Norazhar, Soo Yee Lee, Siti Munirah Mohd Faudzi, Khozirah Shaari
Summary: Christia vespertilionis is an ornamental plant with butterfly-shaped leaves, also known as butterfly wing or rerama in Malay. The green-leafed variety has gained popularity in Malaysia for its medicinal uses, yet there is limited information on its phytochemistry. Detailed analysis revealed a variety of metabolites in the leaf, with flavonoids being the major class. Further research is needed for the potential valorization of this plant as a phytopharmaceutical product.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Chemical
Mohamad Shazeli Che Zain, Soo Yee Lee, Chian Ying Teo, Khozirah Shaari
Summary: This study successfully enriched flavonoid C-glycosides from OPLAH extract using XAD7HP resin, which exhibited the best performance for enrichment. The enriched fractions showed excellent antioxidant activities and may have practical value in producing high value-added products.
Article
Biochemistry & Molecular Biology
Muhammad Afiq Ngadni, Muhammad Tayyab Akhtar, Intan Safinar Ismail, Anis Irfan Norazhar, Soo Yee Lee, Maulidiani Maulidiani, Khozirah Shaari
Summary: The study identified various active constituents in Clitorea ternatea root extract, including clitorienolactones and isoflavonoidal compounds, which showed significant effects on stress-induced zebrafish model. This suggests that the traditional medicinal use of Clitorea ternatea roots in alleviating brain-related conditions may be attributed to these compounds.
Article
Plant Sciences
Nawal Buzgaia, Soo Yee Lee, Yaya Rukayadi, Faridah Abas, Khozirah Shaari
Summary: The research evaluated the antioxidant and alpha-glucosidase inhibitory activities of the Arbutus genus plant, showing its potential medicinal value in treating diabetes and preventing oxidative damage.
Article
Environmental Sciences
Elliyana Nadia Hamidi, Parvaneh Hajeb, Jinap Selamat, Soo Yee Lee, Ahmad Faizal Abdull Razis
Summary: This study evaluated the bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in charcoal-grilled beef and chicken and found that the degree of doneness and fat content of the meats had an impact on the bioaccessibility of PAHs. The stomach had the highest bioaccessibility, followed by the small intestine, and individual PAHs had higher bioaccessibility in grilled beef compared to grilled chicken. Additionally, the bioaccessibility of PAHs increased with the increase in the degree of doneness, and there was a positive correlation between PAHs' bioaccessibility and the fat contents of grilled meat.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Agatha Anak Sibuar, Nur Syahirah Zulkafflee, Jinap Selamat, Mohd Razi Ismail, Soo Yee Lee, Ahmad Faizal Abdull Razis
Summary: This study investigates the contamination of heavy metals in paddy soils and plants in three districts in Perak, Malaysia. The results show that the roots contain the highest concentration of heavy metals, with As being the most concentrated. The accumulation of As, Cu, and Cr in rice grains is observed, and different sampling areas exhibit different compositional patterns of heavy metal contamination. Health risk assessment reveals both noncarcinogenic and carcinogenic risks for local residents.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Nur Syahirah Zulkafflee, Nurul Adillah Mohd Redzuan, Sara Nematbakhsh, Jinap Selamat, Mohd Razi Ismail, Sarva Mangala Praveena, Soo Yee Lee, Ahmad Faizal Abdull Razis
Summary: The study found heavy metal contamination in paddy plants in Kelantan, Malaysia, posing adverse risks to human health. Bioaccumulation of heavy metals was measured using enrichment and translocation factors, and health risk assessment was conducted following USEPA guidelines. The intake of heavy metals through rice ingestion is likely to cause both non-carcinogenic and carcinogenic health risks.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Green & Sustainable Science & Technology
Mohamad Shazeli Che Zain, Jen Xen Yeoh, Soo Yee Lee, Khozirah Shaari
Summary: Six natural deep eutectic solvents (NaDES) were synthesized as extraction solvents for flavonoids from oil palm leaves, showing favorable physicochemical properties and low cytotoxicity. Hydrogen bonding interactions played a significant role in the formation of NaDES, and they have the potential as green solvents for extracting bioactive compounds.
Article
Biochemistry & Molecular Biology
Mohamad Shazeli Che Zain, Jen Xen Yeoh, Soo Yee Lee, Adlin Afzan, Khozirah Shaari
Summary: An innovative method using NaDES combined with ultrasonic assisted extraction was developed to extract valuable antioxidant flavonoids from oil palm leaves. The key factors influencing the extraction efficiency include the heating method, molar ratios, and water contents during NaDES synthesis. Results showed that NaDES extracts could be a more efficient alternative to aqueous methanol for extracting bioactive compounds, with promising potential for applications such as wound healing agents.
Article
Plant Sciences
Muhamad Faris Osman, Soo Yee Lee, Shahrul Razid Sarbini, Siti Munirah Mohd Faudzi, Shamsul Khamis, Badrul Hisyam Zainudin, Khozirah Shaari
Summary: The differences in pungency of Piper betle in Malaysia suggest the presence of several variants. Metabolite profiling of different variants revealed quantitative differences and identified two distinct groups based on DNA sequences. Findings support the existence of two P. betle variants in Malaysia based on chemical profiles.