期刊
NEURON
卷 109, 期 18, 页码 2847-+出版社
CELL PRESS
DOI: 10.1016/j.neuron.2021.07.007
关键词
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资金
- Canadian Institutes of Health Research ( CIHR ) [PJT -162108]
- Canada-Israel Health Research Initiative (CIHR)
- Canada-Israel Health Research Initiative (Israel Science Foundation)
- Canada-Israel Health Research Initiative (International Development Research Centre)
- Canada-Israel Health Research Initiative (Azrieli Foundation) [IDRC 108875]
- FCT [PTDC/BIA-BID/29663/2017]
- Intramural Research Program of the National Eye Institute, NIH [ZIAEY000450]
- Krembil Foundation
- Canada First Research Excellence Fund/Medicine by Design
- Cumming School of Medicine
- Ontario Government Scholarship
- UofT Vision Science Research Program
- Peterborough K.M. HUNTER Charitable Foundation
- Margaret and Howard GAMBLE Research Grant
- Alberta Innovates Health Solutions
- ACHRI/CIHR Training grant
- FNR CORE grant [C15/BM/10397420]
- CIHR Canada HOPE Fellowship
- Frederick Banting and Charles Best Canada
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-BID/29663/2017] Funding Source: FCT
The research reveals that Neurog2 and Ascl1 genes sustain neurogenic continuity and lissencephaly, while double+ NPCs show unique features of multipotency and play a key role in neurogenic symmetry and cortical folding.
Asymmetric neuronal expansion is thought to drive evolutionary transitions between lissencephalic and gyrencephalic cerebral cortices. We report that Neurog2 and Ascl1 proneural genes together sustain neurogenic continuity and lissencephaly in rodent cortices. Using transgenic reporter mice and human cerebral organoids, we found that Neurog2 and Ascl1 expression defines a continuum of four lineage-biased neural progenitor cell (NPC) pools. Double+ NPCs, at the hierarchical apex, are least lineage restricted due to Neurog2-Ascl1 cross-repression and display unique features of multipotency (more open chromatin, complex gene regulatory network, G2 pausing). Strikingly, selectively eliminating double+ NPCs by crossing Neurog2-Ascl1 split-Cre mice with diphtheria toxin-dependent deleterstrains locally disrupts Notch signaling, perturbs neurogenic symmetry, and triggers cortical folding. In support of our discovery that double+ NPCs are Notch-ligand-expressing nichecells that control neurogenic periodicity and cortical folding, NEUROG2, ASCL1, and HES1 transcript distribution is modular (adjacent high/low zones) in gyrencephalic macaque cortices, prefiguring future folds.
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