Article
Biochemistry & Molecular Biology
Kazuki Miura, Takehiro Suzuki, Hongkai Sun, Haruka Takada, Yudai Ishizawa, Hayato Mizuta, Naoshi Dohmae, Siro Simizu
Summary: C-mannosylation of ADAMTS4 at specific sites affects protein processing, intracellular trafficking, secretion, and enzymatic activity. Mutations at certain sites can impair the functions of ADAMTS4.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Steven J. Berardinelli, Andrew R. Sillato, Richard C. Grady, Sanjiv Neupane, Atsuko Ito, Robert S. Haltiwanger, Bernadette C. Holdener
Summary: Thrombospondin 1 (THBS1) is a secreted extracellular matrix glycoprotein with diverse functions. Its modular domains interact with various proteins in the extracellular matrix to regulate cellular and physiological processes. The modifications of THBS1, such as O-linked glucose-fucose disaccharide and C-mannose, may affect its trafficking and function.
Article
Biochemistry & Molecular Biology
Midori Ikezaki, Kazuchika Nishitsuji, Ko Matsumura, Shino Manabe, Yukinao Shibukawa, Yoshinao Wada, Yukishige Ito, Yoshito Ihara
Summary: This study investigated the effects of C-mannosylation on the biological function of WSPW peptides and found that C-mannosylated WSPW peptides could interact with actinin-4, influencing the expression and subcellular localization of E-cadherin in cells.
Review
Biochemistry & Molecular Biology
Samuel L. Crine, K. Ravi Acharya
Summary: C-mannosylation is a rare form of glycosylation that enhances the structural and functional diversity of proteins by forming a carbon-carbon bond with a tryptophan residue. Despite being discovered in 1994, it is still poorly understood and further research is needed to explore its mechanisms and functions.
Article
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara
Summary: C-Mannosylation affects the biological functions of proteins, especially in thrombospondin 1 repeats. C-Mannosylated WSPW peptide inhibits TGF-beta-mediated synaptogenesis by suppressing Smad 2 activation. The molecular details of how C-mannosylation affects the biological functions of WxxW motifs require further study for clarification.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Valainipha Habuddha, Chanyatip Suwannasing, Aticha Buddawong, Kanokwan Seenprachawong, Thitinat Duangchan, Chanyarak Sombutkayasith, Aungkura Supokawej, Wattana Weerachatyanukul, Somluk Asuvapongpatana
Summary: Thrombospondin repeats (TSR) are important peptide domains in many extracellular and transmembrane proteins, interacting with various ligands. The study characterized HdTSR domains in the ADAMTS3 protein of Thai abalone and found that recombinant HdTSR1/2 protein can induce differentiation of MC3T3-E1 cells by upregulating osteogenic markers in a concentration- and time-dependent manner. HdTSR1/2 protein may interact through TGF-beta and activate downstream signaling cascade to trigger biomineralization in differentiated osteoblastic cells.
MARINE BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Yoshito Ihara
Summary: Protein C-mannosylation is a unique type of protein glycosylation where a single α-mannose is attached to the indole C2 of tryptophan through a C-C bond. Dpy-19, DPY19L1, and DPY19L3 are genes encoding C-mannosyltransferases. Consensus motifs of C-mannosylation can be found in thrombospondin type 1 repeat and cytokine receptor type I families, suggesting critical roles of this modification in protein folding, sorting, and secretion.
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Pratik Narain Srivastava, Bandita Nayak, Varun Dewaker, Satish Mishra
Summary: C-Mannosylation is a crucial factor in the function of thrombospondin type I repeat (TSR) domains and plays an essential role in malaria transmission in Plasmodium berghei.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Geng Chen, Yang Yang, Qi-Jia Wu, Liu Cao, Wen Ruan, Changwei Shao, Li Jiang, Peng Tang, Suping Ma, Ao Jiang, Zhen Wang, Kai Wu, Qiangfeng Cliff Zhang, Xiang-Dong Fu, Yu Zhou
Summary: ILF3 plays a negative regulatory role in miRNA biogenesis by directly repressing miRNAs through binding to A/U-enriched motifs, particularly targeting miR-582-3p, which is crucial in the antiviral response.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Nicholas J. Carruthers, Clarissa Strieder-Barboza, Joseph A. Caruso, Carmen G. Flesher, Nicki A. Baker, Samuel A. Kerk, Alexander Ky, Anne P. Ehlers, Oliver A. Varban, Costas A. Lyssiotis, Carey N. Lumeng, Paul M. Stemmer, Robert W. O'Rourke
Summary: Dysfunctional visceral adipose tissue in obesity is associated with type 2 diabetes, with downregulation of proteins related to fatty acid synthesis and mitochondrial function in diabetes, while upregulation of proteins associated with innate immunity and transcriptional regulation. Transcriptome analysis indicated defects in innate inflammation, lipid metabolism, and extracellular matrix function. Shared biological processes impacted by diabetes include complement activation, cell proliferation and migration, ECM organization, lipid metabolism, and gluconeogenesis. The study reveals potential mechanisms of VAT dysfunction in diabetes.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Nele Zoellner, Noemi Coesfeld, Frederik Henry De Vos, Jennifer Denter, Haifeng C. Xu, Elena Zimmer, Birgit Knebel, Hadi Al-Hasani, Sofie Mossner, Philipp A. Lang, Doreen M. Floss, Juergen Scheller
Summary: Researchers found that synthetic type I interferon signaling mainly originates from IFNAR2, rather than IFNAR1, by reformatting the structure of IFNAR1 and IFNAR2.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huilin Yang, Umut Y. Ulge, Alfredo Quijano-Rubio, Zachary J. Bernstein, David R. Maestas, Jung-Ho Chun, Wentao Wang, Jian-Xin Lin, Kevin M. Jude, Srujan Singh, Brian T. Orcutt-Jahns, Peng Li, Jody Mou, Liam Chung, Yun-Huai Kuo, Yasmin H. Ali, Aaron S. Meyer, Warren L. Grayson, Nicola M. Heller, K. Christopher Garcia, Warren J. Leonard, Daniel-Adriano Silva, Jennifer H. Elisseeff, David Baker, Jamie B. Spangler
Summary: In this study, IL-4 cytokine mimetics called Neo-4 were designed based on a de novo engineered IL-2 mimetic scaffold. These cytokines can mimic the physiological functions of IL-4 and offer insights into differential IL-4 signaling through type I versus type II receptors. The design workflow of this study can inform the development of targeted cytokine therapeutics.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Allergy
Anders O. Petersen, Martta Jokinen, Damian R. Plichta, Gerhard Liebisch, Wolfram Gronwald, Katja Dettmer, Peter J. Oefner, Hera Vlamakis, Daniel C. Chung, Annamari Ranki, Ramnik J. Xavier
Summary: The gut microbiome in APS-1 patients is correlated with APS-1 symptoms, and probiotic intervention can alleviate some gastrointestinal symptoms. Furthermore, autoantibodies against cytokines appear to shape the gut microbiome by positively correlating with a taxonomically consistent group of bacteria.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Fisheries
Fan-Xiang Gao, Wei-Jia Lu, Yan Shi, Zhan-Wei Zhao, Li Zhou, Jian-Fang Gui, Zhe Zhao
Summary: This study systematically analyzed IFN genes in pufferfish and identified different types of IFN genes, studying their molecular characteristics and phylogenetic relationships. The results showed that these IFN genes have different expression patterns in various tissues of pufferfish and play multiple roles in antiviral responses. Additionally, specific transcription factor binding sites and induction effects were investigated.
AQUACULTURE REPORTS
(2022)
Article
Immunology
Cody J. Bills, Hongjie Xia, John Yun-Chung Chen, Jason Yeung, Birte K. Kalveram, David Walker, Xuping Xie, Pei-Yong Shi
Summary: Mutations outside the Spike gene in SARS-CoV-2, such as the triple deletion in nonstructural protein 6 (nsp6), can enhance the virus's ability to suppress interferon signaling, thereby affecting its pathogenicity and transmission.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Biotechnology & Applied Microbiology
Yuki Sasaki, Masahiro Komeno, Akihiro Ishiwata, Ayako Horigome, Toshitaka Odamaki, Jin-Zhong Xiao, Katsunori Tanaka, Yukishige Ito, Kanefumi Kitahara, Hisashi Ashida, Kiyotaka Fujita
Summary: Gum arabic arabinogalactan protein (AGP) can be effectively degraded by the cooperative action of GAfase, exo-beta 1,3-galactanase (Bl1,3Gal), and BlArafE, leading to the production of alpha-L-Rhap-(1 -> 4)-beta-D-GlcpA-(1-6)-beta-D-Galp-(1 -> 6)-D-Gal tetrasaccharide. This oligosaccharide can be utilized by commensal bacteria, potentially facilitating cross-feeding interactions between bifidobacteria and other gut microbial groups.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Midori Ikezaki, Kazuchika Nishitsuji, Ko Matsumura, Shino Manabe, Yukinao Shibukawa, Yoshinao Wada, Yukishige Ito, Yoshito Ihara
Summary: This study investigated the effects of C-mannosylation on the biological function of WSPW peptides and found that C-mannosylated WSPW peptides could interact with actinin-4, influencing the expression and subcellular localization of E-cadherin in cells.
Article
Biochemistry & Molecular Biology
Wataru Miyanishi, Makoto Ojika, Dai Akase, Misako Aida, Yasuhiro Igarashi, Yukishige Ito, Yu Nakagawa
Summary: The study found that attachment of 2-aminoethanol to the carboxy group of PRM-A significantly suppressed aggregation, and the prepared amide derivatives not only bound to Man without aggregation but also exhibited antifungal activity.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara
Summary: C-Mannosylation affects the biological functions of proteins, especially in thrombospondin 1 repeats. C-Mannosylated WSPW peptide inhibits TGF-beta-mediated synaptogenesis by suppressing Smad 2 activation. The molecular details of how C-mannosylation affects the biological functions of WxxW motifs require further study for clarification.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Yoshiko Takeda-Uchimura, Midori Ikezaki, Tomoya O. Akama, Kaho Nishioka, Yoshito Ihara, Fabrice Allain, Kazuchika Nishitsuji, Kenji Uchimura
Summary: In this study, it was found that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3 under physiological conditions.
Article
Biochemistry & Molecular Biology
Sayaka Higashi, Yuki Imamura, Takashi Kikuma, Takahiro Matoba, Saya Orita, Yoshiki Yamaguchi, Yukishige Ito, Yoichi Takeda
Summary: The details of the complex formation between SelenoF and HUGT1 in the endoplasmic reticulum glycoprotein quality control system are still unclear. This study used co-transfection and crosslinking experiments to reveal the binding position of SelenoF to HUGT1, further elucidating the function of SelenoF.
Article
Chemistry, Multidisciplinary
Junpei Abe, Yoichi Takeda, Takashi Kikuma, Yasuhiko Kizuka, Hiroyuki Kajiura, Yasuhiro Kajihara, Yukishige Ito
Summary: UDP-Glc:glycoprotein glucosyltransferase (UGGT) plays a central role in maintaining the quality control of correctly folded N-glycoproteins in the endoplasmic reticulum (ER). However, a selective and potent inhibitor against UGGT has not been identified. In this study, we report squaryl group-modified UDP analogs as potential UGGT inhibitors, with one compound showing the highest potency and suggesting a candidate for further optimization.
CHEMICAL COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Yoshito Ihara
Summary: Protein C-mannosylation is a unique type of protein glycosylation where a single α-mannose is attached to the indole C2 of tryptophan through a C-C bond. Dpy-19, DPY19L1, and DPY19L3 are genes encoding C-mannosyltransferases. Consensus motifs of C-mannosylation can be found in thrombospondin type 1 repeat and cytokine receptor type I families, suggesting critical roles of this modification in protein folding, sorting, and secretion.
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Methanee Hiranyakorn, Shogo Iwamoto, Asako Hoshinoo, Ryo Tsumura, Hiroki Takashima, Masahiro Yasunaga, Shino Manabe
Summary: N-Linked glycosylation on IgG has a significant impact on antibody functions. The relationship between N-glycan structure and Fc gamma RIIIa binding affinity affects ADCC activity. The study shows that the N-glycan structure and length on IgGs, Fc fragments, and ADCs influence the retention time in column chromatography, suggesting their impact on Fc gamma RIIIa binding and ADCC activity.
Article
Biochemistry & Molecular Biology
Yahani P. Jayasinghe, Michael T. Banco, Jared J. Lindenberger, Lorenza Favrot, Zuzana Palcekova, Mary Jackson, Shino Manabe, Donald R. Ronning
Summary: This study further characterized the functionality of Mycothiol S-transferase (MST) in Mycobacterium tuberculosis and examined its role in xenobiotic stress. The results showed that MST is stabilized by the binding of Mycothiol (MSH) and the presence of metal ions. However, cell-based experiments failed to provide evidence for the involvement of MST in the processing of rifampicin or isoniazid, suggesting the need for further research to identify the acceptors of the enzyme and better define its biological role.
RSC MEDICINAL CHEMISTRY
(2023)
Meeting Abstract
Oncology
Hiroki Takashima, Yoshikatsu Koga, Shino Manabe, Kazunobu Ohnuki, Ryo Tsumura, Takahiro Anzai, Yang Wang, Xiaojie Yin, Akihiro Nambu, Nozomi Sato, Sachiko Usuda, Hiromitsu Haba, Hirofumi Fujii, Yasuhiro Matsumura, Masahiro Yasunaga
Meeting Abstract
Oncology
Shiqi Yang, Masahiro Yasunaga, Shino Manabe
Article
Biochemistry & Molecular Biology
Shun Maruyama, Kota Sawano, Satoko Amaki, Takehiro Suzuki, Satoru Narita, Kenta Kimura, Takatoshi Arakawa, Chihaya Yamada, Yukishige Ito, Naoshi Dohmae, Kiyotaka Fujita, Akihiro Ishiwata, Shinya Fushinobu
Summary: This study investigated the catalytic mechanism and substrate binding structure of the beta-l-arabinofuranosidase HypBA1 from Bifidobacterium longum. Zn2+ ion and cysteine were found to play key roles in catalysis, and the mode of substrate binding affected the catalytic activity.