Article
Biotechnology & Applied Microbiology
Banakar N. Sahana, M. K. PrasannaKumar, H. B. Mahesh, P. Buela Parivallal, M. E. Puneeth, Chirag Gautam, T. R. Girish, Sailaja Nori, Shrikumar Suryanarayan
Summary: Seaweed extracts can enhance rice plant resistance against fungal pathogens by increasing defense-related enzyme levels, altering defense gene transcription levels, and reducing disease severity, ultimately inducing resistance to blast fungus.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yan Bi, Hui Wang, Xi Yuan, Yuqing Yan, Dayong Li, Fengming Song
Summary: This study reveals the function and regulatory network of a NAC transcription factor, ONAC083, in rice immunity. ONAC083 enhances pathogen-induced defense responses and pattern-triggered immunity, while its knockout strengthens rice immunity against Magnaporthe oryzae. ONAC083 directly activates the transcription of OsRFPH2-6, which negatively regulates rice immunity.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Review
Cell Biology
Quyen Thu Bui, Jeong Hee Hong, Minseok Kwak, Ji Yeon Lee, Peter Chang-Whan Lee
Summary: The ubiquitin-mediated degradation system controls various tumor-promoting processes through the sequential action of E1, E2, and E3 enzymes, with E2 enzymes playing a central role in modulating pathophysiological processes in the tumor microenvironment.
Article
Cell Biology
Xiaoman You, Shanshan Zhu, Haowen Sheng, Zheng Liu, Dan Wang, Min Wang, Xiao Xu, Feng He, Hong Fang, Fan Zhang, Debao Wang, Zeyun Hao, Ruyi Wang, Yinghui Xiao, Jianmin Wan, Guo-Liang Wang, Yuese Ning
Summary: Knockdown of OsPEX5 enhances resistance to rice blast fungus Magnaporthe oryzae. OsPEX5 interacts with E3 ubiquitin ligase APIP6 to regulate plant immunity. Additionally, OsPEX5 interacts with OsALDH2B1 to enhance its repression of defense gene expression.
Article
Biochemistry & Molecular Biology
Muthukumar Elangovan, Jun Ka, Boryeong Pak, Woosoung Choi, Se-Ra Oh, Suk-Won Jin, Yung Joon Yoo
Summary: Ubiquitination plays an important regulatory role in angiogenesis, with E3 enzymes being extensively studied. The role of E2 enzymes in angiogenesis is less known. Through analysis of single-cell RNA sequencing data, UBE2V1 was identified as one of the most abundantly expressed E2s in endothelial cells. Inhibition of UBE2V1 affects endothelial cell proliferation, viability, morphogenesis, and migration, and is critical for FGF2-induced angiogenesis.
Article
Multidisciplinary Sciences
Alejandra Martinez-D'Alto, Xia Yan, Tyler C. Detomasi, Richard I. Sayler, William C. Thomas, Nicholas J. Talbot, Michael A. Marletta
Summary: Blast disease caused by the fungus Magnaporthe oryzae leads to significant crop loss in cereal plants. The study shows that the expression of the putative polysaccharide monooxygenase MoPMO9A is increased during infection. MoPMO9A exhibits activity on cereal-derived mixed β-D-glucans and its domain architecture is different from other characterized AA9 PMOs. The results suggest that MoPMO9A plays a role in MBG degradation during plant infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Muxing Liu, Jiexiong Hu, Ao Zhang, Ying Dai, Weizhong Chen, Yanglan He, Haifeng Zhang, Xiaobo Zheng, Zhengguang Zhang
Summary: This study revealed a novel role of the COPII uncoating factor MoSwa2 in vesicular transport, showing its critical importance in suppressing host immunity and pathogenicity in M. oryzae. MoSwa2 deficiency resulted in impaired secretion of extracellular proteins, affecting the growth of invasive hyphae and the inhibition of host immunity. Additionally, the study identified the extracellular protein MoSef1 as an apoplastic virulent factor that inhibits host immune response.
Article
Fisheries
Jian Zhang, Xiangyi Sun, Xuepeng Li, Shuning Zhang, Xiudan Xu, Jixing Feng
Summary: Many viruses hijack the host's ubiquitin-proteasome system (UPS) for successful infection. This study identified and characterized a protein called SmE2D2 in turbot, which interacts with the RBIV-C1 virus. The results show that SmE2D2 is an E2 ubiquitin-conjugating enzyme that is used by RBIV-C1 to promote efficient replication.
Article
Plant Sciences
Junning Ma, Jean-Benoit Morel, Michael Riemann, Peter Nick
Summary: This study dissects the JA-dependent defense signaling in rice for both compatible and incompatible interactions by using JA-deficient mutants cpm2 and hebiba. The results demonstrate the pivotal role of JA in the basal immunity of rice in its resistance to the Rice Blast Fungus in both types of interactions.
Article
Biochemistry & Molecular Biology
Fumiyo Ikeda
Summary: The ubiquitin-proteasomal system and the autophagy-lysosome system are two major degradation systems in mammalian cells. Ubiquitin not only regulates proteasomal degradation of substrates but also regulates the autophagy pathway. Ubiquitin plays a key role in autophagy regulation by recruiting cargos to phagophore in a ubiquitin-dependent manner.
Article
Plant Sciences
Shobit Thapa, Radha Prasanna, Balasubramanian Ramakrishnan, Himanshu Mahawar, Asha Bharti, Aundy Kumar, Kulandaivelu Velmourougane, Yashbir Singh Shivay, Arun Kumar
Summary: This study highlights the importance of utilizing phyllospheric microbes as change-agents to engineer microbial communities for inhibiting fungal pathogen progression in rice crops. The root-shoot linkages play a critical role in the effective biocontrol of indigenous microbes, applied as soil drench or foliar agents. Different microbial inoculation methods show significant effects on chlorophyll concentrations, enzyme activities, and gene abundances, demonstrating the potential for environmentally friendly disease management strategies.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kazuhide Miyamoto, Atsushi Matsumoto
Summary: In this study, the unique properties of ARFs in E2-binding and auto-ubiquitination were demonstrated in ubiquitination assays. Point mutations of ARF by tryptophan resulted in changes in E2 specificity and Ub chain elongation. ARF mutants interacted with all 11 E2s and promoted auto-ubiquitination, allowing specific detection of E2 activities during ubiquitination.
Article
Biotechnology & Applied Microbiology
Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang, Xinqiong Liu
Summary: The study aimed to investigate the regulation of gene expression in the interaction between rice and Magnaporthe oryzae using gene chip technology. Changes in gene expression at different time points post-inoculation were analyzed, revealing significant differentially expressed genes and identifying new genes related to rice blast. This research may contribute to the discovery of novel resistance genes and improve the understanding of rice blast development mechanisms.
Article
Plant Sciences
Pengcheng Wang, Kai Guo, Qian Su, Jinwu Deng, Xianlong Zhang, Lili Tu
Summary: The E2 enzyme GhUBC2L modulates histone monoubiquitination synergistically with E3 ligase GhUbox8 to control organ size in cotton by affecting the expression of genes related to cell cycle and organ development.
Article
Plant Sciences
Guihua Duan, Chunqin Li, Yanfang Liu, Xiaoqing Ma, Qiong Luo, Jing Yang
Summary: This study utilized UHPLC/Q-TOF-MS to identify altered metabolites in transgenic rice infected with M. oryzae, revealing organic acids, organic oxygen compounds, lipids, and lipid-like molecules. The research showed that carbohydrate and amino acid metabolic pathways were involved in plant defense responses and resistance strengthening. The study also found that the identified metabolites could be utilized as an optimal marker for M.oryzae defense in rice.
Review
Plant Sciences
Qussai Zuriegat, Yuru Zheng, Hong Liu, Zonghua Wang, Yingzi Yun
Summary: Fusarium oxysporum is a soilborne plant pathogen that secretes various virulence factors and must respond to stresses from the environment and host during infection. Research has identified 26 functionally characterized transcription factors in F. oxysporum, with 23 playing important roles in pathogenicity, particularly the largest family being zinc finger TF family.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Microbiology
Yan-Ping Wang, Abdul Waheed, Shi-Ting Liu, Wen-Yang Li, Oswald Nkurikiyimfura, Yahuza Lurwanu, Zonghua Wang, Laura J. Grenville-Briggs, Lina Yang, Luping Zheng, Jiasui Zhan
Summary: Climate change is considered a major threat to society and nature, with UV irradiation identified as the most important environmental genotoxic agent. This study focused on investigating UV adaptation in natural ecosystems, particularly through studying the RAD23 gene in the potato late blight pathogen, Phytophthora infestans. Results showed that UV irradiation drives natural selection in the RAD23 gene, influencing the UV tolerance of P. infestans isolates. The study also highlighted the complex interactions of different climatic factors on species adaptation, emphasizing the need for future research using advanced technologies to understand the collective impact of climate change on ecological functions and resilience.
Editorial Material
Plant Sciences
Xiao-feng Chen, Ji-yu Su, Wei-hong He, Jia-yuan Guo, Jian-qiang Huang, Dan Wang, Nan Yang, Hong-li Hu, Zong-hua Wang, Xiang-ying Wei
Article
Biochemistry & Molecular Biology
Zhenzhen Zhao, Jiangbo Fan, Yu G. Gao, Zonghua Wang, Piao Yang, Yinping Liang, Stephen Opiyo, Ye Xia
Summary: Plants have a prompt immune system to defend against pathogen infections. This study reveals that AHA5 negatively regulates the plant immune response, affecting stomata opening, salicylic acid pathway, callose deposition, and defense-related gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Plant Sciences
Jun Liu, Zonghua Wang
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Congxian Wu, Zhongkun Guo, Meiru Zhang, Huilin Chen, Minghui Peng, Yakubu Saddeeq Abubakar, Huawei Zheng, Yingzi Yun, Wenhui Zheng, Zonghua Wang, Jie Zhou
Summary: Calcium and manganese transporters FgGdt1 and FgPmr1 play important roles in regulating Ca2+ and Mn2+ homeostasis in the Golgi apparatus of the plant pathogenic fungus Fusarium graminearum. Disruption of FgGdt1 or FgPmr1 causes defects in growth, development, and virulence of the fungus, as well as altered deoxynivalenol production. FgGdt1 is specifically involved in Mn2+ transportation and its function is important for the normal physiological activities of F. graminearum.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Lianhu Zhang, Chonglei Shan, Yifan Zhang, Wenjing Miao, Xiaoli Bing, Weigang Kuang, Zonghua Wang, Ruqiang Cui, Stefan Olsson
Summary: This study reveals the important role of protein kinase CK2 in the pathogenesis of the rice pathogen Magnaporthe oryzae. CK2 affects multiple cellular processes, including carbohydrate metabolism, fatty acid metabolism, and amino acid metabolism, as well as the folding and complex formation of proteins. These findings provide insights into the mechanism of CK2 in the infection process of the rice pathogen.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Guanghui Wang, Peng Sun, Zhongjuan Sun, Jindong Zhu, Dan Yu, Zhe Tang, Zonghua Wang, Chenfang Wang, Huawei Zheng
Summary: The SR-like protein Sgh1 in Fusarium graminearum is involved in fungal development, plant infection, and pre-mRNA processing, with its function being regulated by the RS domain. The interaction between Sgh1 and the unique SR-specific kinase Srk1 is verified by yeast-two hybrid and bimolecular fluorescence complementation assays.
Article
Microbiology
Jiandong Bao, Zhe Wang, Meilian Chen, Shijie Chen, Xiaomin Chen, Jiahui Xie, Wei Tang, Huakun Zheng, Zonghua Wang
Summary: The study constructed a pan-genome database of Pyricularia oryzae based on genome data from NCBI, and revealed the population structure of the rice blast fungus. The pan-genome will provide a comprehensive reference for further research on Pyricularia oryzae.
Article
Microbiology
Wilfred M. Anjago, Jules Biregeya, Mingyue Shi, Yixiao Chen, Yupeng Wang, Zonghua Wang, Yonghe Hong, Meilian Chen
Summary: This study found that MoPtc1 and MoPtc2 in the rice blast fungus Magnaporthe oryzae are induced by calcium chloride and play a role in regulating MAPK signaling pathways. MoPtc1 is recruited by the adaptor protein MoNbp2 to deactivate Osm1 under hypoosmotic conditions. Additionally, MoPtc1 and MoPtc2 are localized in different cellular compartments during fungal development.
Article
Plant Sciences
Mengtian Pei, Xuze Xie, Baoyi Peng, Xinchi Chen, Yixuan Chen, Ya Li, Zonghua Wang, Guodong Lu
Summary: In this study, 30 phosphatidylinositol transfer proteins (PITPs) were found in the rice genome, with variations in physicochemical properties, gene structure, conservation domains, and subcellular localization. The promoter region of OsPITPs genes contained hormone response elements such as methyl jasmonate (Me JA) and salicylic acid (SA). Additionally, the expression of certain OsPITPs genes was significantly affected by infection of the rice blast fungus Magnaporthe oryzae, indicating a potential role of OsPITPs in rice innate immunity through the Me JA and SA pathway.
Article
Microbiology
Felix Abah, Yunbo Kuang, Jules Biregeya, Yakubu Saddeeq Abubakar, Zuyun Ye, Zonghua Wang
Summary: In this study, the roles of Pmk1 and Mps1 in Sclerotiophoma versabilis were investigated using genetic and cell biology strategies. The results showed that SvPmk1 and SvMps1 are involved in hyphal development, asexual reproduction, and pathogenesis. The mutants exhibited reduced growth under osmotic stress and hypersensitivity to hydrogen peroxide. This study demonstrates the critical roles of SvPmk1 and SvMps1 in stress resistance, development, and pathogenicity of S. versabilis.
Article
Plant Sciences
Shifu Tian, Bojing Liu, Yanan Shen, Shasha Cao, Yinyan Lai, Guodong Lu, Zonghua Wang, Airong Wang
Summary: This study examined the response of two tomato cultivars, Micro-Tom (MT) and Ailsa Craig (AC), to the fungal pathogen Botrytis cinerea at different time points. The results revealed differences in resistance phenotypes and differential gene expression between the two cultivars. The findings highlight the involvement of various metabolic, regulatory, detoxification, and immune-related processes, as well as the Ca2+ pathway and signaling pathways, in tomatoes' defense against B. cinerea. Transcription factors such as WRKY, MYB, NAC, and AUX/IAA families were also found to play crucial regulatory roles.
Article
Plant Sciences
Yanqiong Chen, Yingfeng Lin, Shubiao Zhang, Zhongyuan Lin, Songbiao Chen, Zonghua Wang
Summary: This study identified the HAK gene family in quinoa and investigated their expression patterns and responses to abiotic stresses, revealing their potential roles in salt and alkali tolerance.
Editorial Material
Microbiology
Baohua Wang, Yakubu Saddeeq Abubakar, Zonghua Wang