期刊
MOLECULAR ONCOLOGY
卷 15, 期 11, 页码 3109-3124出版社
WILEY
DOI: 10.1002/1878-0261.13072
关键词
acetaldehyde; alcohol; esophageal squamous cell carcinoma; FANCD2; Fanconi anemia; genomic instability
类别
资金
- NIH [F31-AA027133, R01-AA026297, P30-CA013696]
- W. W. Smith Charitable Trust [C1706]
- Drexel University College of Medicine
The study reveals that acetaldehyde causes DNA damage and impedes replication fork progression, leading to genomic instability in esophageal epithelial cells. FANCD2, which triggers apoptosis upon acetaldehyde exposure and prevents genomic instability in esophageal SCCs, plays a critical role in the replication abnormalities induced by acetaldehyde.
Individuals with Fanconi anemia (FA), a rare genetic bone marrow failure syndrome, have an increased risk of young-onset head and neck squamous cell carcinomas (SCCs) and esophageal SCC. The FA DNA repair pathway is activated upon DNA damage induced by acetaldehyde, a chief alcohol metabolite and one of the major carcinogens in humans. However, the molecular basis of acetaldehyde-induced genomic instability in SCCs of the head and neck and of the esophagus in FA remains elusive. Here, we report the effects of acetaldehyde on replication stress response in esophageal epithelial cells (keratinocytes). Acetaldehyde-exposed esophageal keratinocytes displayed accumulation of DNA damage foci consisting of 53BP1 and BRCA1. At physiologically relevant concentrations, acetaldehyde activated the ATR-Chk1 pathway, leading to S- and G2/M-phase delay with accumulation of the FA complementation group D2 protein (FANCD2) at the sites of DNA synthesis, suggesting that acetaldehyde impedes replication fork progression. Consistently, depletion of the replication fork protection protein Timeless led to elevated DNA damage upon acetaldehyde exposure. Furthermore, FANCD2 depletion exacerbated replication abnormalities, elevated DNA damage, and led to apoptotic cell death, indicating that FANCD2 prevents acetaldehyde-induced genomic instability in esophageal keratinocytes. These observations contribute to our understanding of the mechanisms that drive genomic instability in FA patients and alcohol-related carcinogenesis, thereby providing a translational implication in the development of more effective therapies for SCCs.
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