Article
Biochemistry & Molecular Biology
Huihui Wang, Hao Zhang, Zhirong Sun, Wankun Chen, Changhong Miao
Summary: The research showed that GABABR1 inhibits the migration and invasion of CRC cells by suppressing EMT and the Hippo/YAP1 pathway, indicating that GABABR1 could be a potential therapeutic target for CRC.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wei-Ting Kuo, Matthew A. Odenwald, Jerrold R. Turner, Li Zuo
Summary: Epithelial cells are the first line of defense in mucosal defense. The tight junction, formed by interactions between claudins, zonula occludens, and tight junction-associated MARVEL protein (TAMP) families, plays a key role in sealing the paracellular space and preventing leakage. In addition to their canonical functions in barrier formation, ZO-1 and occludin also have essential noncanonical functions, including regulating apoptosis and proliferation, facilitating viral entry, and organizing specialized epithelial structures.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Cell Biology
Weiyan Zou, Yi Zhang, Guangfu Bai, Jialu Zhuang, Lin Wei, Zishu Wang, Meiqun Sun, Junbin Wang
Summary: siRNA-induced CD44 knockdown inhibits the biological behaviors of colorectal cancer stem cells (CSCs), including proliferation, migration, invasion, and tumorigenesis. It also promotes apoptosis, induces cell-cycle arrest, increases sensitivity to chemotherapy, and inhibits metastasis by suppressing epithelial-mesenchymal transition (EMT).
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Boyang Fan, Chaojing Zheng, Ning Wang, Zewen Chang, Yunxiao Liu, Chunlin Wang, Jun Xiang, Yangbao Tao, Guiyu Wang, Qian Zhang
Summary: The study identifies a novel circRNA, circSTK3, which is significantly upregulated in colorectal cancer (CRC) tissues and is associated with decreased survival. Functional assays show that circSTK3 promotes cell migration and invasion, and gene expression analysis reveals its involvement in CRC metastasis. The study also uncovers that CTCF transcriptionally regulates circSTK3. These findings highlight the functional and prognostic roles of circSTK3 and demonstrate the importance of circRNAs in metastasis.
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Andrea Abaurrea, Angela M. Araujo, Maria M. Caffarel
Summary: Epithelial-mesenchymal plasticity (EMP) is crucial in cancer development, promoting the acquisition of mesenchymal features in cancer cells through cytokine signal transduction pathways, such as the interleukin 6 (IL-6) family. However, the effect may depend heavily on the context.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
William W. Du, Javeria Qadir, Kevin Y. Du, Yu Chen, Nan Wu, Burton B. Yang
Summary: Current studies have shown that nuclear actin plays a significant role in the regulation of epithelial-mesenchymal transition (EMT). Different binding partners for nuclear F-actin and G-actin have been discovered, which respectively modulate EMT-promoting and EMT-repressing transcriptional events. Mechanistically, nuclear F-actin enhances the expression and stability of proteins involved in EMT promotion, while nuclear G-actin increases the expression and stability of proteins involved in EMT repression. The association between nuclear actin and EMT suggests that targeting nuclear actin dynamics for dysregulated EMT is a promising area for further study.
Article
Anatomy & Morphology
Xiangjun Lu, Jian Shen, Siyuan Huang, Haitao Wang, Dongdong Liu
Summary: This study demonstrates that the low expression of CLEC3B in LUAD cells leads to decreased cell adhesion capability, enhanced EMT, migration, and invasion, which is associated with the p53 signaling pathway.
Article
Multidisciplinary Sciences
Wenli Zang, Junchao Liu, Fengxue Geng, Dongjuan Liu, Shuwei Zhang, Yuchao Li, Yaping Pan
Summary: This study found that butyrate can promote the migration and invasion of oral squamous cell carcinoma (OSCC) cells by inducing epithelial-mesenchymal transition (EMT), indicating that butyrate may contribute to the metastasis of OSCC.
Article
Pathology
Zhe Cheng, Suwen Li, Jiaqi Yuan, Yongdong Li, Shan Cheng, Shilu Huang, Jun Dong
Summary: In this study, the expression patterns of HDAC1 and its impact on prognosis in glioblastoma (GBM) patients were evaluated based on TCGA and CGGA databases. High expression of HDAC1 was found to be associated with poor prognosis in GBM, and its interaction with the EMT-related protein VIM was explored. Functional enrichment analysis further revealed the enrichment of cancer markers in GBM tissues with high HDAC1 expression. In vitro and in vivo experiments demonstrated that an HDAC1 inhibitor could inhibit the EMT process and affect the invasive ability of GBM cells.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Biochemistry & Molecular Biology
Eleonora Sarantelli, Apostolis Mourkakis, Lefteris C. Zacharia, Andreas Stylianou, Vasiliki Gkretsi
Summary: As metastasis is a major cause of cancer-related deaths, understanding the cellular and molecular events involved in cancer cell migration and invasion is crucial for developing novel anti-metastatic therapies. Fascin-1, an actin-bundling protein, plays a fundamental role in cell migration processes. It is significantly elevated in most cancers and its high expression is associated with aggressive disease and poor prognosis. Recent studies show that Fascin-1 is critically involved in metastasis and suggest it as a promising target for anti-metastatic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Qin Yu, Jianzhang Wang, Tiantian Li, Xinyue Guo, Shaojie Ding, Xuan Che, Libo Zhu, Yangying Peng, Xinxin Xu, Gen Zou, Xinmei Zhang
Summary: RON, a receptor tyrosine kinase, promotes endometriosis development by facilitating epithelial-mesenchymal transition (EMT) of endometrial epithelial cells. Inhibition or knockdown of RON expression suppresses the migration and invasion of endometrial epithelial cells. Targeting RON may offer a new therapeutic approach for endometriosis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Huaguo Liang, Zexin Chen, Ruihui Yang, Qingsong Huang, Hongmei Chen, Wanting Chen, Li Zou, Peng Wei, Shijie Wei, Yongxia Yang, Yongli Zhang
Summary: The polyphenolic compound Methyl gallate (MG) found in plants can inhibit the proliferation, migration, and invasion of HCC cells both in vitro and in vivo. The therapeutic mechanism of MG potentially involves the regulation of the AMPK/NF-kappa B pathway, which influences the epithelial-mesenchymal transition and MMP expression.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Oncology
Nayden G. Naydenov, Susana Lechuga, Emina H. Huang, Andrei I. Ivanov
Summary: Colorectal cancer (CRC) is a deadly disease that often presents at an advanced stage, with few interventions available. Myosins, a family of actin motor proteins, play crucial roles in regulating cell proliferation, differentiation, and motility. Altered expression or mutations of myosins have been documented in CRC, but their specific roles in cancer development remain poorly understood.
Article
Oncology
Min Hee Yang, Chakrabhavi Dhananjaya Mohan, Amudha Deivasigamani, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Kanchugarakoppal S. Rangappa, Sang Hoon Jung, Hyejin Ko, Kam Man Hui, Gautam Sethi, Kwang Seok Ahn
Summary: Procaine inhibits the EMT process and c-Met-dependent signaling cascades, suggesting its potential as an anti-metastatic agent for HCC.
Article
Dermatology
Karthic Swaminathan, Andrew Campbell, Vassilis Papalazarou, Farah Jaber-Hijazi, Colin Nixon, Ewan McGhee, Douglas Strathdee, Owen J. Sansom, Laura M. Machesky
Summary: The study reveals that NCKAP1 plays a crucial role in the progression and maintenance of tissue integrity in BRAF(V600E)-driven melanoma, with its depletion delaying tumor onset and progression, reducing proliferation and tumor growth, and leading to increased collagen deposition and immune infiltration.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shashi Prakash Singh, Peter A. Thomason, Sergio Lilla, Matthias Schaks, Qing Tang, Bruce L. Goode, Laura M. Machesky, Klemens Rottner, Robert H. Insall
Article
Developmental Biology
Vassilis Papalazarou, Karthic Swaminathan, Farah Jaber-Hijazi, Heather Spence, Ines Lahmann, Colin Nixon, Manuel Salmeron-Sanchez, Hans-Henning Arnold, Klemens Rottner, Laura M. Machesky
Article
Multidisciplinary Sciences
Luke Tweedy, Peter A. Thomason, Peggy I. Paschke, Kirsty Martin, Laura M. Machesky, Michele Zagnoni, Robert H. Insall
Review
Cell Biology
James Drew, Laura M. Machesky
Summary: The dissemination of cancer cells from primary tumors to metastatic sites is a crucial step in cancer progression. The interaction between cancer cells and the specific microenvironments in target organs influences the success of metastases. Understanding the roles of organ-specific microenvironments and extracellular matrix dynamics in different stages of metastasis may lead to targeted therapies against metastatic disease.
DISEASE MODELS & MECHANISMS
(2021)
Article
Cell Biology
Anh Hoang Le, Tamas Yelland, Nikki R. Paul, Loic Fort, Savvas Nikolaou, Shehab Ismail, Laura M. Machesky
Summary: The study reveals that CYRI-A is a key regulator of macropinosome formation and integrin internalization, being transiently recruited to nascent macropinosomes and dependent on PI3K and RAC1 activity for its function.
JOURNAL OF CELL BIOLOGY
(2021)
Editorial Material
Cell Biology
Michael F. Olson, Laura M. Machesky
Summary: The study demonstrates that MICAL2 mediates methionine oxidation of ARP3B, disrupting ARP2/3 complexes and causing disassembly of branched actin filaments.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Rehabilitation
Jagjit Mankelow, Cormac G. Ryan, Hayley Morris, Douglas Lauchlan, Christopher Seenan, Paul Taylor, Denis Martin
Summary: This study tracked changes in physiotherapy students' attitudes and beliefs towards people with chronic pain over a four-year period in Scotland. The findings showed significant improvements in attitudes and beliefs, with more noticeable improvements in the final two years.
PHYSIOTHERAPY THEORY AND PRACTICE
(2022)
Editorial Material
Cell Biology
Laura M. Machesky, Robert H. Insall
Summary: The study demonstrates how mechanical effects can regulate the WAVE complex and the importance of forces generated by actin filaments to control new actin generation at the leading edge.
NATURE CELL BIOLOGY
(2021)
Article
Cell Biology
Vasileios Papalazarou, James Drew, Amelie Juin, Heather J. Spence, Jamie Whitelaw, Colin Nixon, Manuel Salmeron-Sanchez, Laura M. Machesky
Summary: Pancreatic cancer is a deadly and highly metastatic disease. This study explores the influence of stiff extracellular matrix (ECM) on pancreatic cancer cell behavior and suggests that pancreatic cancer cells can be reprogrammed when exposed to a soft microenvironment. Collagen VI plays a key role in this process.
JOURNAL OF CELL SCIENCE
(2022)
Article
Oncology
Emma L. Wilkinson, Louise C. Brennan, Olivia J. Harrison, Zoe Crane-Smith, Philippe Gautier, Margaret A. Keighren, Peter Budd, Karthic Swaminathan, Laura M. Machesky, Sarah L. Allinson, Ian J. Jackson, Richard L. Mort
Summary: Genetic approaches are crucial for studying melanocytes and melanoma. Current methods for labeling melanocytes in mice have limitations, but researchers have successfully generated a targeted multicistronic allele using homologous recombination in mouse embryonic stem cells, allowing specific labeling and live imaging of melanocytes. This transgenic allele has been demonstrated to be useful in embryonic and adult tissues.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Laura M. Machesky
Summary: Cells use actin-based protrusions to migrate, sample their environment, and take up liquids and particles. Lamellipodia and macropinocytic cups are involved in cell migration and uptake of surrounding substances. The regulation between these functions is not well understood.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Editorial Material
Cell Biology
Laura M. Machesky
Summary: Programmed cell death (PCD) helps cells to benefit the surviving organism. A recent study shows that cells can program their death by inducing extensive disulfide bonding of the actin cytoskeleton in response to an imbalance of cystine, a raw material for glutathione production.
NATURE CELL BIOLOGY
(2023)
Article
Pathology
Hayley T. Morris, William R. Bamlet, Gina L. Razidlo, Laura M. Machesky
Summary: The expression of FSCN1 and EMT-TFs SLUG/SNAI2 and TWIST1 in pancreatic adenocarcinoma (PDAC) is associated with the development of the disease. High expression of FSCN1 is correlated with poorer survival. The expression of EMT-TFs is not associated with survival, but is correlated with FSCN1 expression.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Editorial Material
Biology
Laura Machesky
