期刊
METABOLIC ENGINEERING
卷 67, 期 -, 页码 387-395出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2021.08.001
关键词
Carbon conservation; CO2 fixation; Coculture; 13C-fingerprinting
资金
- ASU LightWorks
- IGERT fellowship - National Science Foundation [1144616]
- Graduate Research Support Fellowship from ASU
- National Science Foundation [CBET-1705409]
- Division Of Graduate Education
- Direct For Education and Human Resources [1144616] Funding Source: National Science Foundation
The study successfully improved carbon conservation efficiency by exploiting the natural heterogeneity of lignocellulosic sugars mixtures and using specific fractions; a coculture-coproduction system of two catabolically orthogonal Escherichia coli strains for biofuel production was demonstrated.
Carbon loss in the form of CO2 is an intrinsic and persistent challenge faced during conventional and advanced biofuel production from biomass feedstocks. Current mechanisms for increasing carbon conservation typically require the provision of reduced co-substrates as additional reducing equivalents. This need can be circumvented, however, by exploiting the natural heterogeneity of lignocellulosic sugars mixtures and strategically using specific fractions to drive complementary CO2 emitting vs. CO2 fixing pathways. As a demonstration of concept, a coculture-coproduction system was developed by pairing two catabolically orthogonal Escherichia coli strains; one converting glucose to ethanol (G2E) and the other xylose to succinate (X2S). C-13-labeling studies reveled that G2E + X2S cocultures were capable of recycling 24% of all evolved CO2 and achieved a carbon conservation efficiency of 77%; significantly higher than the 64% achieved when all sugars are instead converted to just ethanol. In addition to CO2 exchange, the latent exchange of pyruvate between strains was discovered, along with significant carbon rearrangement within X2S.
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