Review
Biochemistry & Molecular Biology
Irina Lyapina, Vadim Ivanov, Igor Fesenko
Summary: This review provides a comprehensive overview of the mechanisms of generation and properties of peptidomes across different organisms, proposing three major peptide groups. The composition of peptide pools identified by mass-spectrometry analysis in human cells, plants, yeast and bacteria is compared and discussed, along with the functions of different peptide groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kelly A. Dingess, Inge Gazi, Henk W. P. van den Toorn, Marko Mank, Bernd Stahl, Karli R. Reiding, Albert J. R. Heck
Summary: Human milk contains beta-casein, a protein that undergoes post-translational modifications such as phosphorylation and glycosylation. A study observed changes in phosphorylation and previously unexplored O-glycosylation of beta-casein in two donors over 16 weeks of lactation, indicating the importance of these modifications on antimicrobial peptides derived from beta-casein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Marie-Louise Heymich, Laura Nissl, Dominik Hahn, Matthias Noll, Monika Pischetsrieder
Summary: The study found that antimicrobial peptides Leg1 and Leg2 from chickpea have the potential to be used as preservatives against bacterial, fungal, and oxidative spoilage in food and other products, without cytotoxic effects on human cells. Additionally, Leg2 showed antioxidative activity by radical scavenging and reducing mechanisms.
Article
Biology
Mariena J. A. van der Plas, Jun Cai, Jitka Petrlova, Karim Saleh, Sven Kjellstrom, Artur Schmidtchen
Summary: The study characterized the peptidome of human wound fluids using mass-spectrometry analyses for the first time, revealing a higher degree of peptides in sterile post-surgical wound fluids compared to human plasma. The unique peptide patterns identified in uninfected healing wounds and Staphylococcus aureus-infected wounds suggest the potential of using these peptides as biomarkers for wound healing and infection.
Article
Infectious Diseases
Anna Ramata-Stunda, Martins Boroduskis, Elza Kaktina, Liene Patetko, Uldis Kalnenieks, Zane Lasa, Marta Rubina, Inese Strazdina, Gints Kalnins, Reinis Rutkis
Summary: Skin and soft tissue infections (SSTIs) and acne are common skin conditions. Antibiotic resistance in pathogens causing SSTIs and acne is increasing, making treatment challenging. This study designed and compared six new antimicrobial peptides (AMPs) with existing ones. RP556 showed broad-spectrum antibacterial activity without cytotoxicity or hemolysis and stimulated IL-10 production. Four new AMPs showed specific activity against Cutibacterium acne below cytotoxic or hemolytic concentrations.
Article
Chemistry, Medicinal
Chun Yuan, Xiaoying Zheng, Kunna Liu, Wenbin Yuan, Yang Zhang, Fan Mao, Yongbo Bao
Summary: The bactericidal activity of predicted peptides from Nautilus pompilius hemocyanin was analyzed, with mutant peptide NpHM4 showing high antibacterial activity and typical antimicrobial peptide characteristics. NpHM4 exhibited significant antibacterial activity against Gram-negative bacteria without cytotoxicity to mammalian cells.
Article
Biochemistry & Molecular Biology
Elisabeth Bankell, Sara Dahl, Olof Gidlof, Daniel Svensson, Bengt-Olof Nilsson
Summary: LL-37 reduces human MG63 cell viability, induces apoptosis, and increases plasma membrane permeabilization. LL-37-induced cell death is caspase-independent.
Article
Microbiology
Rayssa Oliveira Araujo, Michel Lopes Leite, Thais Tavares Baraviera Dutra, Nicolau Brito da Cunha, Taia Maria Berto Rezende, Marcelo Henrique Soller Ramada, Simoni Campos Dias
Summary: Antimicrobial peptides (AMPs) are components of the innate immune system and can be found in the venom of various animals. In this study, analogs of the peptide Cupiennin-1a were designed to reduce cytotoxicity and improve antimicrobial and antitumor activity. The results showed that the analogs displayed antimicrobial activity against bacterial and fungal strains, and had decreased cytotoxicity compared to the original peptide. They also showed antitumor activity against breast adenocarcinoma cell lines. The rational design of these analogs demonstrated their potential for biotechnological applications.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ingvill Pedersen Saebo, Magnar Bjoras, Henrik Franzyk, Emily Helgesen, James Alexander Booth
Summary: In vitro determination of hemolytic properties is commonly used to evaluate the cytotoxicity of chemicals, drugs, or blood-contacting medical devices or materials. However, there is significant variation in the protocols used in different experimental setups, leading to difficulties in interpretation and comparison of results. This study examines the impact of different variables on the outcome of the assay and proposes an optimized protocol to standardize future hemolysis studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Ceramics
Kitae Park, Kambiz Sadeghi, Sarinthip Thanakkasaranee, Ye-In Park, Junsoo Park, Ki-Ho Nam, Haksoo Han, Jongchul Seo
Summary: Thermally calcined oyster shells were studied as biocompatible antimicrobial agents, with CaO identified as the main antimicrobial component effective against both Gram-negative and Gram-positive bacteria. Calcined oyster shells treated at 750 degrees C showed the best antimicrobial potency.
INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY
(2021)
Article
Biophysics
Emanuelle D. Freitas, Rogerio A. Rataglioli, Josephine Oshodi, Marisa M. Beppu
Summary: The coronavirus pandemic has highlighted the importance of bioactive materials in mitigating viral infections. Bioinspired antimicrobial peptides (AMPs) have emerged as suitable building blocks for antimicrobial coatings due to their versatile design and environmentally friendly features. This review discusses the advances and opportunities in using AMPs to create virucidal coatings, including the fundamental characteristics of peptide structure, advances in coating medical devices, and the role of different materials in designing antiviral coatings.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Andrea Cerrato, Sara Elsa Aita, Anna Laura Capriotti, Chiara Cavaliere, Angela Michela Immacolata Montone, Carmela Maria Montone, Aldo Lagana
Summary: In this study, a suspect screening approach based on high-resolution mass spectrometry was used to comprehensively characterize the short endogenous peptidome in dry-cured ham samples. A total of 236 short peptide sequences, including 173 natural short peptides and 63 sequences containing non-proteinogenic amino acids, were tentatively identified. Several modified short peptides, such as pyroglutamination and lactoyl conjugation, were also observed, suggesting reactions occurring over time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Sergio Montserrat-de la Paz, Fernando Rivero-Pino, Alvaro Villanueva, Rocio Toscano-Sanchez, Maria E. Martin, Francisco Millan, Maria C. Millan-Linares
Summary: Hemp seeds have gained interest due to their high nutritional composition and antioxidant properties. In this study, ten hemp protein hydrolysates were obtained and evaluated for their antioxidant activity. Shorter peptide sequences obtained with Flavourzyme were found to react with free radicals more easily. Peptides KNAIYTPH, EERPGHF, and KNGMMAPH were proposed to contribute highly to the antioxidant activity.
Article
Biochemical Research Methods
Jingmei Liao, Min Gao, Yelin Ding, Qirui Bi, Dongdong Huang, Xiaoxiao Luo, Peilei Yang, Yun Li, Yong Huang, Changliang Yao, Jianqing Zhang, Wenlong Wei, Zhenwei Li, De-an Guo
Summary: Animal-derived drugs are crucial in folk medicine globally, yet their chemical compositions are poorly understood. This lack of knowledge contributes to the low quality control of animal-derived drugs and leads to a chaotic market. In this study, a strategy combining proteogenomics and pseudotargeted peptidomics was developed to characterize natural peptides and identify signature peptides for four leech species. The strategy significantly improved peptide identification and successfully screened differential and signature peptides. Furthermore, a dynamic multiple reaction monitoring method based on these signature peptides revealed the origin of commercial samples and Tongxinluo capsules.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Pieter M. Dekker, Sjef Boeren, Johannes B. van Goudoever, Jacques J. M. Vervoort, Kasper A. Hettinga
Summary: The study found significant interindividual variation in proteins and peptides in human milk, and identified distinct characteristics in different protein and peptide clusters through weighted correlation network analysis. Several associations between different data sets and specific sample characteristics were also discovered.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Physics, Multidisciplinary
Tian-Tian Xie, Jun Wang, Fei-Bo Du, Yang Yu, Yan-Fei Cai, Er-Yuan Feng, Fei Hou, Zhi-Wei Liu
Summary: GGNMOS devices are widely used as power clamps in order to protect semiconductor devices from electrostatic discharge stress. We conducted I-V characterization tests on GGNMOS devices used as power clamps in complementary metal-oxide-semiconductor circuits, and found a special effect when switching ESD paths under different impact energies. This effect can lead to unexpected latch-up or pre-failure phenomenon in applications with large capacitances from power supply to power ground, and requires urgent analysis and resolution. Transmission-line-pulse, human-body-modal, and light-emission tests were performed to investigate the root cause.
Article
Dermatology
Lin Liu, Chen Liang, Yuzhou Xue, Tingqiao Chen, Yangmei Chen, Yufan Lan, Jiamei Wen, Xinyi Shao, Jin Chen
Summary: This study aimed to develop an accurate diagnostic system for melasma based on deep learning. By evaluating the performances of different deep learning models, the researchers found that using multiple modes of images in the VISIA system significantly improved the network performance for melasma diagnosis.
DERMATOLOGY AND THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Peiyu Fang, Jiawen Liu, Huanyu Liu, Gang Yu, Pengxiang Fu, Bingwu Wang, Jianglan Qu, Yanyi Huang, Zuqiang Bian, Zhiwei Liu
Summary: Doublet emission from open-shell molecules has research and application value. However, the understanding of the photoluminescence mechanism of open-shell molecules is less than that of closed-shell molecules, making the design of efficient doublet emission systems challenging. This study presents a cerium(III) complex Ce(CzPhTp)(3) with a new luminescence mechanism of delayed doublet emission, representing the first example with metal-centered delayed photoluminescence. The energy gap between the doublet and triplet excited states is reduced by coordinating the inner and outer spheres, promoting efficient energy transfer and activating delayed emission.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Respiratory System
Hongwu Wang, Wen Li, Zikai Wang, Liangan Chen, Guoxiang Lai, Faguang Jin, Mingyao Ke, Jiayuan Sun, Jie Zhang, Baosong Xie, Nan Zhang, Wangping Li, Hongmei Zhou, Xiaoping Wang, Dianjie Lin, Yunzhi Zhou, Huaping Zhang, Dongmei Li, Changhui Wang, Xiaolian Song, Juan Wang, Shiman Wu, Junyong Yang, Lei Zhang, Meimei Tao, Yiming Zeng, Heng Zou, Hui Li, Fujie Song, Zhengbu Sha, Qiang Tan, Minghua Cong, Hanping Shi, Zhina Wang, Xinwei Han, Lingfei Luo, Hongming Ma, Gang Wu, Zhiqiang Wang, Xiaochuan Liu, Weiping Wu, Lishan Zhang, Yongan Ye, Guangying Zhu
Summary: Acquired digestive-respiratory tract fistulas are abnormal communication between the respiratory tract and digestive tract caused by various benign or malignant diseases, which result in the presence of alimentary canal contents in the respiratory tract. Although advanced closure techniques have been explored, there is a lack of large-scale evidence-based medical data to guide clinical diagnosis and treatment. The guidelines update the understanding of the disease and introduce respiratory and digestive stent implantation as the most important and effective treatment. The guidelines also provide a detailed review of current evidence and recommendations for stent selection, implantation methods, postoperative management, and efficacy evaluation.
CLINICAL RESPIRATORY JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Feng Zhao, Jiang Huang, Xinyu Cui, Jiqiang Wang, Tianying Xiong
Summary: In this research, a silicon-aluminizing diffusion coating, consisting of homogenous Ti(Al, Si)(3) phase, was successfully prepared on ?-TiAl alloy. The oxidation behavior of the coating was investigated at high temperatures of 900, 950, and 1000 degrees C. The results showed that the coating exhibited improved oxidation resistance at these temperatures due to the formation of a protective oxide scale.
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
(2023)
Article
Physics, Multidisciplinary
F. P. An, W. D. Bai, A. B. Balantekin, M. Bishai, S. Blyth, G. F. Cao, J. Cao, J. F. Chang, Y. Chang, H. S. Chen, H. Y. Chen, S. M. Chen, Y. Chen, Y. X. Chen, Z. Y. Chen, J. Cheng, Z. K. Cheng, J. J. Cherwinka, M. C. Chu, J. P. Cummings, O. Dalager, F. S. Deng, Y. Y. Ding, X. Y. Ding, M. V. Diwan, T. Dohnal, D. Dolzhikov, J. Dove, H. Y. Duyang, D. A. Dwyer, J. P. Gallo, M. Gonchar, G. H. Gong, H. Gong, W. Q. Gu, J. Y. Guo, L. Guo, X. H. Guo, Y. H. Guo, Z. Guo, R. W. Hackenburg, Y. Han, S. Hans, M. He, K. M. Heeger, Y. K. Heng, Y. K. Hor, Y. B. Hsiung, B. Z. Hu, J. R. Hu, T. Hu, Z. J. Hu, H. X. Huang, J. H. Huang, X. T. Huang, Y. B. Huang, P. Huber, D. E. Jaffe, K. L. Jen, X. L. Ji, X. P. Ji, R. A. Johnson, D. Jones, L. Kang, S. H. Kettell, S. Kohn, M. Kramer, T. J. Langford, J. Lee, J. H. C. Lee, R. T. Lei, R. Leitner, J. K. C. Leung, F. Li, H. L. Li, J. J. Li, Q. J. Li, R. H. Li, S. Li, S. C. Li, W. D. Li, X. N. Li, X. Q. Li, Y. F. Li, Z. B. Li, H. Liang, C. J. Lin, G. L. Lin, S. Lin, J. J. Ling, J. M. Link, L. Littenberg, B. R. Littlejohn, J. C. Liu, J. L. Liu, J. X. Liu, C. Lu, H. Q. Lu, K. B. Luk, B. Z. Ma, X. B. Ma, X. Y. Ma, Y. Q. Ma, R. C. Mandujano, C. Marshall, K. T. McDonald, R. D. McKeown, Y. Meng, J. Napolitano, D. Naumov, E. Naumova, T. M. T. Nguyen, J. P. Ochoa-Ricoux, A. Olshevskiy, H. -R. Pan, J. Park, S. Patton, J. C. Peng, C. S. J. Pun, F. Z. Qi, M. Qi, X. Qian, N. Raper, J. Ren, C. Morales Reveco, R. Rosero, B. Roskovec, X. C. Ruan, B. Russell, H. Steiner, J. L. Sun, T. Tmej, K. Treskov, W. -H. Tse, C. E. Tull, B. Viren, V. Vorobel, C. H. Wang, J. Wang, M. Wang, N. Y. Wang, R. G. Wang, W. Wang, X. Wang, Y. Wang, Y. F. Wang, Z. Wang, Z. Wang, Z. M. Wang, H. Y. Wei, L. H. Wei, W. Wei, L. J. Wen, K. Whisnant, C. G. White, H. L. H. Wong, E. Worcester, D. R. Wu, Q. Wu, W. J. Wu, D. M. Xia, Z. Q. Xie, Z. Z. Xing, H. K. Xu, J. L. Xu, T. Xu, T. Xue, C. G. Yang, L. Yang, Y. Z. Yang, H. F. Yao, M. Ye, M. Yeh, B. L. Young, H. Z. Yu, Z. Y. Yu, B. B. Yue, V. Zavadskyi, S. Zeng, Y. Zeng, L. Zhan, C. Zhang, F. Y. Zhang, H. H. Zhang, J. L. Zhang, J. W. Zhang, Q. M. Zhang, S. Q. Zhang, X. T. Zhang, Y. M. Zhang, Y. X. Zhang, Y. Y. Zhang, Z. J. Zhang, Z. P. Zhang, Z. Y. Zhang, J. Zhao, R. Z. Zhao, L. Zhou, H. L. Zhuang, J. H. Zou
Summary: We present a new determination of the smallest neutrino mixing angle 013 and the mass-squared difference Delta m232 using a final sample of 5.55 x 106 inverse beta-decay (IBD) candidates with the final-state neutron captured on gadolinium. Compared to the previous Daya Bay results, selection of IBD candidates has been optimized, energy calibration refined, and treatment of back-grounds further improved. The resulting oscillation parameters are sin22013 = 0.0851 +/- 0.0024, Delta m232 = (2.466 +/- 0.060) x 10-3 eV2 for the normal mass ordering or Delta m232 = -(2.571 +/- 0.060) x 10-3 eV2 for the inverted mass ordering.
PHYSICAL REVIEW LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Zhijun Li, Xiaowen Lu, Rufang Zhao, Siqi Ji, Mingyang Zhang, J. Hugh Horton, Yang Wang, Qian Xu, Junfa Zhu
Summary: Researchers have developed an efficient molten-salt-assisted approach to create atomically dispersed cobalt atoms supported over vanadium pentoxide layered material. This cobalt single-atom catalyst achieves extraordinary catalytic efficiency in the dehydrogenative coupling reaction of alcohols and amines, with high selectivity and conversion. Density functional theory calculations confirm the optimized coordination environment and strong metal-support interaction contribute significantly to the activation of reactants.
Review
Immunology
Ru-Yue Chen, Yun Zhu, Yun-Yan Shen, Qin-Ying Xu, Han-Yun Tang, Ning-Xun Cui, Lu Jiang, Xiao-Mei Dai, Wei-Qing Chen, Qiang Lin, Xiao-Zhong Li
Summary: Programmed cell death 1 receptor (PD-1) and its ligands play a crucial role in immune tolerance, immune homeostasis, and immunosuppression. The PD-1 pathway has been implicated in various diseases, including cancer and autoimmune diseases, and PD-1 blockades have been approved for cancer treatment. This comprehensive review focuses on the structure and expression of PD-1, PD-L1, and PD-L2, the diverse biological functions of PD-1 signaling in health and immune-related diseases, and the immune-related adverse events associated with PD-1 and PD-L1 inhibitors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Fan Mao, Xiaoying Zheng, Nai-Kei Wong, Wenjie Yi, Jingchen Song, Shiwei Fu, Zhiming Xiang, Shu Xiao, Yongbo Bao, Ziniu Yu, Yang Zhang
Summary: This study reveals the regulatory mechanisms of the Hippo pathway in immune responses of Crassostrea hongkongensis hemocytes, highlighting its importance in maintaining immune homeostasis and orchestrating hemocytic functions. It also demonstrates the divergence of the Hippo pathway's roles in marine invertebrate immunity from mammalian observations, indicating the need for further comparative studies across species.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Neurosciences
Yonglu Wang, Lingxi Xu, Hui Fang, Fei Wang, Tianshu Gao, Qingyao Zhu, Gongkai Jiao, Xiaoyan Ke
Summary: The study aims to identify patterns of social dysfunction in adolescents with autism spectrum disorder (ASD), investigate the potential linkage between social brain networks and stereotyped behavior, and explore potential targets of non-invasive nerve stimulation to improve social disorders. The results showed that the functional connectivity between social-related brain regions and many other brain regions was significantly weakened in the ASD group compared to the typically developing group. The findings suggest that social network dysfunction may be related to stereotyped behavior.
Article
Engineering, Electrical & Electronic
Minghui Cao, Jiang-Wen Xiao, Hongliang Fang, Zhi-Wei Liu, Yan-Wu Wang
Summary: This paper proposes a new probability density forecasting framework for residential load forecasting. The framework combines weighted external features and internal load curves to build a similar-day dataset, and uses an exclusive predictor for point forecasting. Probability density forecasting is achieved through kernel density estimation and calibration. Comparisons with other methods demonstrate the efficiency and superiority of the proposed framework in both point and probability density forecasting.
INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS
(2023)
Article
Chemistry, Analytical
Yu Chen, Ying Chen, Hong-Chao Yi, Hui-Wen Gu, Xiao-Li Yin, Duo -Lin Xiang, Peng Zou
Summary: In this study, a smart DNA-modified Au/CuMOF bioconjugate material (ssDNA-Au/CuMOF) was synthesized and applied in the dual-mode determination of S. aureus. The method demonstrated a broad linear range, low detection limit, and good selectivity, making it suitable for detecting S. aureus in complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Peixuan Cui, Zhiwei Liu, Xincheng Cui, Yingge Li, Dongxing Du
Summary: This study experimentally investigates the effect of water on the Minimum Miscibility Pressure (MMP) of the CO2/n-Hexadecane (n-C16H34) system, and finds that the presence of water decreases the MMP values of the CO2/n-C16H34 system.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Neurosciences
Sumei Fan, Xinqi Cheng, Pingping Zhang, Yuanyin Wang, Liecheng Wang, Juan Cheng
Summary: The sedative effects of dexmedetomidine are not completely understood, but it has been found to activate NA(-) and NA(+) neurons in the VLPO, which may explain its unique sedative properties with rapid arousal.
Article
Environmental Sciences
Nian Wu, Lin Tao, Kunming Tian, Xia Wang, Caidie He, Songlin An, Yingkuan Tian, Xiang Liu, Wei Chen, Haonan Zhang, Pei Xu, Dengqing Liao, Juan Liao, Linglu Wang, Derong Fang, Zhongmei Hu, Hongyu Yuan, Jingyi Huang, Xiaoshan Chen, Li Zhang, Xiaohui Hou, Rong Zeng, Xingyan Liu, Shimin Xiong, Yan Xie, Yijun Liu, Quan Li, Xubo Shen, Yuanzhong Zhou, Xuejun Shang
Summary: This study investigated the urinary concentrations of nine phthalic acid ester (PAE) metabolites in pregnant women in China. The results showed cumulative exposure to PAEs among pregnant women, and residential characteristics were found to be associated with urinary PAE metabolite concentrations. Living in buildings and using coal/kerosene/wood/wheat straw as domestic fuel were associated with higher health risks.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)