4.7 Article

Green extraction using deep eutectic solvents and antioxidant activities of flavonoids from two fruits of Rubia species

期刊

LWT-FOOD SCIENCE AND TECHNOLOGY
卷 148, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.lwt.2021.111708

关键词

Rubia fruit; Flavonoids; Green extraction; Response surface methodology; Antioxidant activity

资金

  1. Forestry Science Discipline Construction Project of Tibet Agriculture AMP
  2. Animal Husbandry University [2020001]
  3. Natural Science Foundation of Heilongjiang Province [LH2019C006]

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Natural deep eutectic solvents were investigated as green extraction solvents for the ultrasound-assisted extraction of flavonoids from Rubia truppeliana and R. cordifolia fruit. Optimal extraction conditions were determined using a Box-Behnken experimental design, resulting in extracts with excellent antioxidant activities, demonstrating the eco-friendly nature of this method.
Natural deep eutectic solvents (DESs) were investigated as potential green extraction solvents in the ultrasoundassisted extraction (UAE) of flavonoids from Rubia truppeliana and R. cordifolia fruit. Lactic acid was selected as the optimal hydrogen bond donor for five solvents in the screening of DESs. To optimize the extraction of Rubia fruit flavonoids using DES and UAE, a Box-Behnken experimental design was developed. The optimal conditions were: liquid-solid ratio, 30; extraction temperature, 70 degrees C; and extraction time, 40 min. The corresponding yields were 49.41 mg/g for total flavonoids from Rubia truppeliana (TFT) and 55.15 mg/g for that of Rubia cordifolia (TFC). The extracts obtained under the optimum extraction conditions showed excellent antioxidant activities in DPPH, ABTS, and FRAP assays. The results demonstrated that the extraction using DES and UAE is an ecofriendly method for the extraction of flavonoids from Rubia fruit.

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