Article
Chemistry, Analytical
Peng Gao, Ling Wang, Lin Zhao, Ying-yuan Lu, Ke-wu Zeng, Ming-bo Zhao, Yong Jiang, Peng-fei Tu, Xiao-yu Guo
Summary: A total of 49 limonoids derivatives, including three new ones, were isolated from the root bark of Dictamnus dasycarpus and tested for anti-inflammatory activities. The newly identified compound, DAS, showed strong anti-inflammatory effects by suppressing NF-κB signaling pathways and inhibiting the release of inflammatory cytokines. Our results suggest that obacunone derivatives play an important role in the anti-inflammatory effects of Baixianpi.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Medicinal
Qian-Ru Zhang, Meng -Yin Tian, Xin Li, Ning Zhang, Yu Chen, Su -Juan Wang
Summary: Nine undescribed protolimonoids were isolated from the root bark of Dictamnus dasycarpus Turcz, along with five known compounds. Their structures were determined by extensive spectroscopic analysis. Compounds 4-8, featuring an undescribed 22,25-epoxytirucallene part, had their absolute configuration established by single-crystal X-ray diffraction. These compounds might provide evidence for the degradation of protolimonoids to limonoids, bridging an oxidative cleavage biogenetic pathway between these structurally diverse triterpenoids. However, none of them exhibited anti-inflammatory, hepatoprotective, or monoamine oxidase B inhibitory activity.
Article
Pharmacology & Pharmacy
Qiong Chen, Ting Zhou, Jun-jie Yuan, Xiao-yi Xiong, Xue-hui Liu, Zong-ming Qiu, Lin-lin Hu, Hui Lu, Qian He, Chang Liu, Qing-wu Yang
Summary: This study investigated the metabolic alterations during ischemia-reperfusion injury using metabolomic analysis. A total of 82 differentially expressed metabolites were identified, with some significantly elevated in the reperfusion group. Metabolomics pathway analysis revealed the importance of glycerophospholipid metabolism, linoleic acid metabolism, pyrimidine metabolism, and galactose metabolism in the response to cerebral ischemia-reperfusion. This study provides important insights into the metabolic changes induced by cerebral ischemia-reperfusion.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mengting Liu, Zhiqin Liu, Zhuang Dong, Xianglin Zou, Jianguo Zeng, Zihui Yang
Summary: This study investigated the metabolites of sanguinarine (SAN) in rats. Using UPLC-Q-TOF-MS/MS technology, 12 metabolites were identified, and their metabolic pathways were inferred. This research is important for further understanding the metabolic mechanism of SAN in vivo and in vitro.
Article
Chemistry, Applied
Xi Juan Zhao, Peng Mei Guo, Wen Hui Pang, Tao Tan, Yao Hai Zhang, Bi Ning Jiao
Summary: This study investigated the secondary metabolites of Jindou kumquat and identified the dominant characteristic metabolites. The findings provide a basis for further evaluation and utilization of Fortunella germplasms.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Physical
Yiying Liu, Dongxue Cao, Yue Liang, Hongli Zhou, Yahong Wang
Summary: In this study, infrared spectral analysis was used to analyze Dictamnus dasycarpus Turcz (DdT) of different growth years, revealing changes in chemical composition and antioxidant activity as growth time increased, providing a technical basis for identification and extraction.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Agriculture, Multidisciplinary
Mingjin Li, Yunzhi Zhang, Jiawei Yan, Fengjiao Ding, Chunmei Chen, Sitong Zhong, Minxuan Li, Yanyu Zhu, Panpan Yue, Pengchun Li, Shijun You, Shan Jin
Summary: Beauty tea was made from fresh leaves of Camellia sinensis Jinxuan cultivar, which were punctured by tea green leafhoppers to different extents. The study identified differential metabolites related to tea polyphenols, lipids, and saccharides in the fresh leaves and dry beauty tea. The quality of beauty tea was influenced by the levels of leafhopper puncturing through the presence of specific characteristic volatiles.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Huifang Zhao, Yang Liu, Yuqing Huang, Qiyu Liang, Shengguan Cai, Guoping Zhang
Summary: This study used ultraperformance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry technology to investigate the temporal changes of metabolites during malting in different barley varieties and compare the metabolite profiles in kilned malt. The results showed that specific metabolic events occurred during steeping, with Franklin barley variety showing earlier and greater changes. Additionally, there were differences in metabolite composition between malt barley and non-malt barley in the kilned malt samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xiuyun Li, Chenglun Zhang, Enhui Wu, Liang Han, Xiangliang Deng, Zhongfeng Shi
Summary: This study aimed to investigate the effects and mechanisms of action of Osthole (OST) in breast cancer 4T1 cells. The results showed that OST inhibited cell proliferation, blocked cells from remaining in S-phase, and induced apoptosis. OST mainly affected phospholipid biosynthesis, methyl histidine metabolism, pyrimidine metabolism, and beta-oxidation of very long-chain fatty acid pathways in 4T1 cells, possibly via inhibition of mTOR/SREBP1/FASN signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Yan-ping Xing, Yue-yue Song, Yan-yun Yang, Che Bian, Wen-xiao Men, He-fei Xue, Liang Xu, Ting-guo Kang
Summary: In this study, the complete chloroplast genome of D. dasycarpus was reported, providing detailed information on its length, structure, and gene annotation. The phylogenetic tree revealed the evolutionary relationship between D. dasycarpus and related species.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Biochemical Research Methods
Su Guan, Kun Liu, Zimeng Liu, Liping Zhou, Bingjie Jia, Zichen Wang, Yao Nie, Xuyu Zhang
Summary: This study identified potential metabolic biomarkers to improve the diagnostic accuracy of sepsis, through metabolomic profiling and regression analysis. A model incorporating ten metabolic biomarkers and traditional physiological indicators showed better differentiation for sepsis diagnosis. The study suggests metabolomic profiling could be an effective tool for sepsis diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Luyao Zhao, Xiaoliang Duan, Hongbin Liu, Dong Zhang, Qian Wang, Jianlei Liu, Hui Sun
Summary: Through lipidomics analysis, the distinction between Wuchang Daohuaxiang (DHX) rice and other nine rice varieties was identified and verified using 26 potential biomarkers. The developed discrimination method achieved an accuracy of 97.14%. The results provide supplementary tools for supervision departments and industries.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Medical Laboratory Technology
Zheng Zhong, Yukai Huang, Qidang Huang, Shaoling Zheng, Zhixiang Huang, Weiming Deng, Tianwang Li
Summary: This study investigated the serum metabolic profiling of gout patients compared to healthy controls using UPLC-Q-TOF/MS, revealing significant differences in metabolites between the two groups. Bilirubin was identified as a potential biomarker for gout, and primary bile acid biosynthesis was suggested as a novel metabolic pathway.
CLINICA CHIMICA ACTA
(2021)
Article
Integrative & Complementary Medicine
Liao Cui-Ping, Liu Xing-Chao, Dong Shi-Qi, An Ming, Zhao Lu, Zhang Ai-Jie, Liu Jian-Feng, Hou Wen-Bin, Fan Hui-Rong, Liu Chang-Xiao
Summary: The study showed that the metabolism pathways of Berberis amurensis extracts (BAEs) differed between normal rats and pseudo germ-free rats, indicating that intestinal bacteria have an influence on the metabolism of BAEs.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Chemistry, Applied
E. Sun, Xuan Li, Fengjuan Xu, Mingyu Li, Ke Ding, Ling Wang, Yingjie Wei, Xiaobin Jia
Summary: In this study, an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was used to analyze the metabolites of Sagittatoside B in rats. A total of 17 metabolites were detected or tentatively identified from rat plasma, bile, urine, and feces. The major metabolic pathways of Sagittatoside B in rats involved hydrolysis, hydrogenation, hydroxylation, dehydrogenation, demethylation, decarbonylation, and conjugation with glucuronic acid and different sugars. This research provides valuable insights into the metabolism of Sagittatoside B and serves as a reference for the metabolism of active flavonoid compounds.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)