期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 72, 期 20, 页码 7049-7066出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erab331
关键词
Epigenetics; FLC; flowering; histone; methyltransferase; methylase; NMD; SDG40; UPF
资金
- National Research Foundation (NRF) of Korea - Korean government [NRF-2017R1A2B3009624]
- Samsung Science and Technology Foundation [SSTF-BA1602-12]
Mutations in UPF1 and UPF3 cause late flowering in Arabidopsis in a temperature- and photoperiod-independent manner by increasing FLC mRNA levels, with NMD regulating FLC epigenetically through SDG40 to modulate flowering time.
The nonsense-mediated mRNA decay (NMD) surveillance system clears aberrant mRNAs from the cell, thus preventing the accumulation of truncated proteins. Although loss of the core NMD proteins UP-FRAMESHIFT1 (UPF1) and UPF3 leads to late flowering in Arabidopsis, the underlying mechanism remains elusive. Here, we showed that mutations in UPF1 and UPF3 cause temperature- and photoperiod-independent late flowering. Expression analyses revealed high FLOWERING LOCUS C (FLC) mRNA levels in upf mutants; in agreement with this, the flc mutation strongly suppressed the late flowering of upf mutants. Vernalization accelerated flowering of upf mutants in a temperature-independent manner. FLC transcript levels rose in wild-type plants upon NMD inhibition. In upf mutants, we observed increased enrichment of H3K4me3 and reduced enrichment of H3K27me3 in FLC chromatin. Transcriptome analyses showed that SET DOMAIN GROUP 40 (SDG40) mRNA levels increased in upf mutants, and the SDG40 transcript underwent NMD-coupled alternative splicing, suggesting that SDG40 affects flowering time in upf mutants. Furthermore, NMD directly regulated SDG40 transcript stability. The sdg40 mutants showed decreased H3K4me3 and increased H3K27me3 levels in FLC chromatin, flowered early, and rescued the late flowering of upf mutants. Taken together, these results suggest that NMD epigenetically regulates FLC through SDG40 to modulate flowering time in Arabidopsis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据