4.7 Article

MdUGT88F1-mediated phloridzin biosynthesis coordinates carbon and nitrogen accumulation in apple

期刊

JOURNAL OF EXPERIMENTAL BOTANY
卷 73, 期 3, 页码 886-902

出版社

OXFORD UNIV PRESS
DOI: 10.1093/jxb/erab410

关键词

Apple; carbon-nitrogen interaction; chloroplast; phloridzin; tricarboxylic acid cycle

资金

  1. National Key Research and Development Program of China [2018YFD1000300]
  2. National Natural Science Foundation of China [32001985]
  3. China Postdoctoral Science Foundation [2020M673513]
  4. Earmarked Fund for China Agriculture Research System [CARS-27]

向作者/读者索取更多资源

MdUGT88F1-mediated phloridzin biosynthesis in apple shoots plays a crucial role in sustaining chloroplast development and photosynthetic carbon fixation by maintaining MdGLK1/2 expression, while interfering with the ammonium assimilation pathway through metabolic competition, leading to chlorophyll deficiency and light sensitivity.
The high accumulation of phloridzin makes apple (Malus domestica) unique in the plant kingdom, which suggests a vital role of its biosynthesis in physiological processes. In our previous study, silencing MdUGT88F1 (a key UDP-GLUCOSE: PHLORETIN 2'-O-GLUCOSYLTRANSFERASE gene) revealed the importance of phloridzin biosynthesis in apple development and Valsa canker resistance. Here, results from MdUGT88F1-silenced lines showed that phloridzin biosynthesis was indispensable for normal chloroplast development and photosynthetic carbon fixation by maintaining MdGLK1/2 (GOLDEN2-like1/2) expression. Interestingly, increased phloridzin biosynthesis did not affect plant (or chloroplast) development, but reduced nitrogen accumulation, leading to chlorophyll deficiency, light sensitivity, and sugar accumulation in MdUGT88F1-overexpressing apple lines. Further analysis revealed that MdUGT88F1-mediated phloridzin biosynthesis negatively regulated the cytosolic glutamine synthetase1-asparagine synthetase-asparaginase (GS1-AS-ASPG) pathway of ammonium assimilation and limited chlorophyll synthesis in apple shoots. The interference of phloridzin biosynthesis in the GS1-AS-ASPG pathway was also assumed to be associated with its limitation of the carbon skeleton of ammonium assimilation through metabolic competition with the tricarboxylic acid cycle. Taken together, our findings shed light on the role of MdUGT88F1-mediated phloridzin biosynthesis in the coordination between carbon and nitrogen accumulation in apple trees. MdUGT88F1-mediated phloridzin biosynthesis in apple shoots sustains chloroplast development and photosynthetic carbon fixation by maintaining MdGLK1/2 expression, and interferes with ammonium assimilation through metabolic competition with the tricarboxylic acid cycle.

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