期刊
FOOD CHEMISTRY
卷 194, 期 -, 页码 723-732出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.08.073
关键词
Skeletonema menzelii; Growth; Total lipid; Polyunsaturated fatty acids (PUFA); Eicosapentaenoic acid (EPA)
资金
- Nonprofit Research Project for the State Oceanic Administration [E00580135100]
- Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University [xkzsc1407]
- K.C. Wong Magna Fund in Ningbo University
Polyunsaturated fatty acids (PUFA) and eicosapentaenoic acid (EPA) are essential for the health of aquaculture organisms and human beings. A total of 9 species of diatoms were screened and two strains of Skeletonema menzelii were selected for further study due to their high growth rates, PUFA and EPA contents. The culture conditions for the yield of biomass, total lipid, EPA and PUFA were optimised. The quickest growth rates (0.28-0.41 d(-1)) were achieved with moderate supplement of nitrogen, phosphorus, iron and silicon. The accumulation of total lipid (17.85-22.70% of dry weight) benefitted from deprivation of nitrogen, phosphorus and silicon, but a moderate iron supplement. Highest PUFA (38.26-50.48% of total fatty acids) or EPA (14.26-18.39% of total fatty acids) contents were observed under high nitrogen and phosphorus supplement, moderate available iron but deprivation of silicon. These findings make tangible contributions to culture S. menzelii for commercial production of PUFA or EPA. (C) 2015 Elsevier Ltd. All rights reserved.
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