Article
Biochemical Research Methods
Isabel Sanz-Vicente, Irina Rivero, Lucia Marcuello, Maria Pilar Montano, Susana de Marcos, Javier Galban
Summary: Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) were immobilized on cellulose to create disposable biosensors for histamine determination. The enzymatic reaction produced a pink spot through the oxidation of AR. By using a smartphone to measure the G (green) color coordinate, histamine can be quantified in the presence of other biogenic amines in a range of concentrations, with a low limit of detection. The method was successfully applied to determine tyramine and histamine in a tuna extract, showing good results compared to the reference HPLC-MS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Anastasios Papavasileiou, Maria G. Trachioti, Jan Hrbac, Mamas Prodromidis
Summary: A modified sensor has been developed for the determination of guanine and adenine in unspiked human whole saliva, with high sensitivity and fast response time. The sensor effectively addresses biofouling issues through a simple assay protocol, achieving a recovery rate of 96-108%.
Article
Chemistry, Multidisciplinary
Sunil Kumar Mahobiya, Sapna Balayan, Nidhi Chauhan, Manika Khanuja, Naresh K. Kuchhal, S. S. Islam, Utkarsh Jain
Summary: Diabetes is a global threat that leads to various diseases. Traditional methods for diagnosing diabetes based on glycated hemoglobin level have limitations. A new electrochemical biosensor has been developed in this study to rapidly and accurately detect glycated hemoglobin protein, showing potential application value.
Article
Chemistry, Analytical
Bohdan Josypcuk, Jan Langmaier, Sofiia Tvorynska
Summary: Silver amalgam screen-printed electrodes (AgA-SPEs) were designed, prepared, and tested as representatives among SPEs for measuring at high negative potentials, showing superior PHE values compared to traditional silver-SPEs. The practical application of AgA-SPEs was demonstrated by analyzing iodine content in table salt, with consistent results as declared by the manufacturer.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Elena Alberto, Julio Bastos-Arrieta, Clara Perez-Rafols, Nuria Serrano, M. Silvia Diaz-Cruz, Jose Manuel Diaz-Cruz
Summary: A DPV method using SPCEs was developed for the determination of SMX, showing a good detection limit (15 μg/L) and a wide linear range (50-600 μg/L) with good repeatability and reproducibility. The method was successfully applied to the analysis of a spiked tap water sample, demonstrating high reproducibility and good trueness. Additional considerations were made on the pH-dependent electrochemical oxidation of SMX and the role of the free terminal amino group in oxidation.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Analytical
Giti Paimard, Ehsan Ghasali, Mireia Baeza
Summary: As electrochemical measuring instruments, screen-printed electrodes (SPEs) are simple, inexpensive, and rapid to analyze substances with high reproducibility, sensitivity, and accuracy. The latest research has focused on expanding SPE electrochemical biosensors, such as aptasensors, immunosensors, DNA sensors, and enzymatic biosensors, to identify, distinguish, and quantify various biocompounds. This review discusses the use of different nanomaterials in manufacturing on-screen electrode methods and strategies for stable diagnosis of biorecognition elements in the future.
Article
Environmental Sciences
Ting Zhang, Juan Han, Houjin Zhang
Summary: A novel electrochemical method was developed for rapid screening of saline-alkali tolerant bacteria using a hydrogel/gold nanoparticles-modified screen-printed electrode. Compared to traditional bacterial culture methods, this method has the clear advantages of rapidity, high efficiency, and low cost.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Gul Sirin Ustabasi, Clara Perez-Rafols, Nuria Serrano, Jose Manuel Diaz-Cruz
Summary: The simultaneous determination of iron and copper using an unmodified screen printed carbon electrode and the complexation with o-phenanthroline assisted by the reducing agent ferrocyanide was successfully achieved. This method has low detection limits and a wide linear response range, allowing for the avoidance of interference between iron and copper and the simultaneous determination of low and high metal concentrations typically found in the environment. Analysis of a certified wastewater reference material confirmed the precision and accuracy of the proposed method.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Mustafa Sen, Merve Oguz, Ipek Avci
Summary: A non-toxic, flexible, low-cost, and disposable multiwalled carbon nanotube (MWCNT)-based screen-printed electrode (SPE) was developed for non-invasive health monitoring applications. The electrode demonstrated excellent electrochemical behavior and is expected to contribute to wearable sensor technology.
Review
Biotechnology & Applied Microbiology
Gang Liang, Zhaoying He, Jianhui Zhen, Hao Tian, Lianfeng Ai, Ligang Pan, Wenwen Gong
Summary: Periodically excessive use of pesticides in agricultural production has led to ecotoxicity and environmental pollution. Biosensing technique is a complementary tool to classical chromatographic methods for rapid, in situ analysis. Screen-printed electrode (SPE) is a widely used method for pesticides detection due to its low cost, easy integration, versatility, and portability. This review provides an overview of the screen-printing technique and SPE, including their definition, preparation procedure, advantages, and discusses the utilization of SPE in detecting pesticides using various bioreceptors and nanomaterials. The sensing principles, analytical performance, advantages, and shortcomings of SPE-based sensors are summarized, and the current challenges and future directions for SPE and SPE-based electrochemical sensors for pollutants are outlined.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Chemistry, Analytical
Jerson Gonzalez-Hernandez, Guillermo Moya-Alvarado, Ana Lorena Alvarado-Gamez, Roberto Urcuyo, Miriam Barquero-Quiros, Maria Julia Arcos-Martinez
Summary: Fentanyl, a synthetic opioid used for severe pain treatment, has become a growing threat to public health due to its increasing illegal recreational use. This study proposes a novel enzymatic biosensor for the rapid and reliable determination of fentanyl in urine samples, using adsorptive-stripping cyclic voltammetry. The biosensor showed high sensitivity, wide linearity range, and low detection limits, making it an efficient device for fentanyl detection.
Article
Multidisciplinary Sciences
Hend. S. S. Magar, Rabeay Y. A. Hassan, Mohammed Nooredeen Abbas
Summary: A new electrochemical impedimetric sensor for direct detection of urea was developed using nanostructured screen-printed electrodes modified with CuO/Co3O4 @MWCNTs. The sensor exhibited a picomolar sensitivity and high selectivity for urea detection, with a linear concentration range of 10(-12)-10(-2) M and a lower detection limit of 0.223 pM (S/N = 5). This non-enzymatic sensor represents a significant advancement in urea detection due to its simplicity, portability, and low-cost fabrication.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Prachi Janjani, Upasana Bhardwaj, Ragini Gupta, Himmat Singh Kushwaha
Summary: This study presents a novel strategy to fabricate an electrochemical sensor based on a bimetallic Mn2+/Fe3+ metal-organic framework (MnFe-MOF) for the direct determination of organophosphate pesticide chlorpyrifos. The sensor showed low detection limit, wide linearity range, excellent repeatability, and anti-interference performance. It successfully recovered the spiked pesticide in real-sample matrices.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Paula Clares, Clara Perez-Rafols, Nuria Serrano, Jose Manuel Diaz-Cruz
Summary: In this work, a simple, fast, sensitive, and low-cost method using a screen-printed carbon electrode (SPCE) was developed for the simultaneous determination of AA, PA, DX, and CF in both pharmaceutical formulations and environmental samples. The method exhibited linear dependence between the oxidative peak current and concentration, with low detection limits and good recoveries.
Article
Chemistry, Multidisciplinary
Daniel A. Smith, Kate Simpson, Matteo Lo Cicero, Lucy J. Newbury, Philip Nicholas, Donald J. Fraser, Nigel Caiger, James E. Redman, Timothy Bowen
Summary: This paper introduces a straightforward electrochemical method for quantifying urinary miRNA using disposable biosensors to distinguish between DKD patients and control subjects. Aberrant miRNA expression has been observed in several major human disorders, and a urinary miRNA signature for DKD has been identified. The method shows great clinical and commercial potential for quantitative miRNA detection in liquid biopsies and may serve as a platform for non-invasive high-throughput screening.
Article
Chemistry, Analytical
J. Bujes-Garrido, D. Izquierdo-Bote, A. Heras, A. Colina, M. J. Arcos-Martinez
ANALYTICA CHIMICA ACTA
(2018)
Article
Chemistry, Analytical
F. Pires, M. Julia Arcos-Martinez, A. Cristina Dias-Cabral, Juan C. Vidal, Juan R. Castillo
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2018)
Article
Chemistry, Analytical
B. Molinero-Abad, D. Izquierdo, L. Perez, I. Escudero, M. J. Arcos-Martinez
Article
Chemistry, Analytical
Hugo Cunha-Silva, M. Julia Arcos-Martinez
Article
Chemistry, Analytical
Hugo Cunha-Silva, M. Julia Arcos-Martinez
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Chemistry, Analytical
Olga Dominguez-Renedo, A. Marta Navarro-Cunado, Eduardo Ventas-Romay, M. Asuncion Alonso-Lomillo
Article
Chemistry, Analytical
Olga Dominguez-Renedo, A. Marta Navarro-Cunado, Victor Arnaiz-Lozano, M. Asuncion Alonso-Lomillo
Article
Electrochemistry
M. Asuncion Alonso-Lomillo, Sheila Lopez-Gil, F. Javier del Campo Garcia, Olga Dominguez-Renedo
Summary: An environmentally friendly electrochemical sensor was developed for the detection of mercaptans in wine samples, using glassy carbon and screen-printed carbon electrodes modified with gold nanoparticles. The sensitivity and selectivity of the sensor were enhanced due to a strong covalent bond formed between sulfur and gold atoms. The reduction of ethanethiol was found to be dependent on both pH and adsorption time.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Jerson Gonzalez-Hernandez, Colby Edward Ott, Maria Julia Arcos-Martinez, Alvaro Colina, Aranzazu Heras, Ana Lorena Alvarado-Gamez, Roberto Urcuyo, Luis E. Arroyo-Mora
Summary: This study explores the spectroelectrochemical response of synthetic cathinones using cyclic voltammetry and Raman spectroscopy for the first time. The proposed method allows for rapid and reliable determination of these substances, making it applicable for forensic analysis of real drug samples and spiked synthetic urine.
Article
Chemistry, Analytical
Paula Portugal-Gomez, M. Asuncion Alonso-Lomillo, Olga Dominguez-Renedo
Summary: This work demonstrates the potential of modified screen-printed carbon electrodes for sensitive and selective detection of 4-ethylphenol in wine. The study compares gold nanoparticles and fullerene C60 as modified sensors, and optimizes the pH, deposition time, and working temperature to achieve the best performance. The activated C60/SPCE shows the highest detection capability under the optimal measurement conditions.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Olga Dominguez-Renedo, A. Marta Navarro-Cunado, M. Asuncion Alonso-Lomillo
Summary: Cholesterol is an important biomarker for diseases such as heart and coronary artery diseases or arteriosclerosis, and its fast determination in blood is crucial for early diagnosis. Electrochemical sensors and biosensors have emerged as potential tools for selective and sensitive detection of cholesterol, combining the analytical advantages of electrochemical techniques with modified electrodes' selective recognition abilities. This review focuses on the development of electrochemical sensors for cholesterol, distinguishing between enzymatic biosensors and non-enzymatic systems, with emphasis on lab-on-a-chip devices. Different electrode modification procedures and the role of functional materials are also discussed.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
M. Asuncion Alonso-Lomillo, Olga Dominguez-Renedo
Summary: This work presents a molecularly imprinted (MIP) sensor for the determination of ethanethiol. The MIP/GCE sensor showed a linear range of 6.1-32.4 mg L-1 with good detection capability and reproducibility. The sensitivity of the sensor was enhanced by incorporating gold nano-particles. The sensor was successfully applied to determine ethanethiol in spiked wine samples.
Article
Chemistry, Analytical
Paula Portugal-Gomez, A. Marta Navarro-Cunado, M. Asuncion Alonso-Lomillo, Olga Dominguez-Renedo
Summary: This article describes an electrochemical method for determining 4-ethylguaiacol and its application in wine analysis. Modified screen-printed carbon electrodes (AC(60)/SPCEs) with fullerene C-60 (C-60) have shown high efficiency in this analysis. Under optimized conditions, AC(60)/SPCEs demonstrated a linear range of 200 to 1000 μg/L for 4-ethylguaiacol determination, with a reproducibility of 7.6% and a detection capability (CCb) value of 200 μg/L. The selectivity of AC(60)/SPCE sensors was evaluated in the presence of potentially interfering compounds, and their practical applicability in analyzing different wine samples was demonstrated with recoveries ranging from 96% to 106%.
Article
Chemistry, Analytical
Paula Portugal-Gomez, Olga Dominguez-Renedo, M. Asuncion Alonso-Lomillo
Summary: This study reports the use of different cobalt phthalocyanine/nanomaterials-based sensors for the headspace detection of volatile thiols. The best performance was achieved by using carbon sensors modified with cobalt phthalocyanine. The optimized amperometric procedure showed good reproducibility, capability of detection, and decision limit for ethanethiol quantification in white and red wines.
Review
Chemistry, Analytical
Olga Dominguez-Renedo, A. Marta Navarro-Cunado, M. Elena Melendez-Alvarez, M. Asuncion Alonso-Lomillo
Summary: The development of electrochemical sensors for analyzing various analytes in wine has been driven by the need to ensure its quality in terms of taste and food safety, as well as compliance with regulations. Certain components in wine can affect its stability and organoleptic properties, and some may have harmful effects on consumers. Therefore, early diagnosis is crucial in wine samples. Enzymatic and non-enzymatic electrochemical sensors, utilizing nanostructured materials or chemically modified electrodes, have been explored to enhance sensitivity and selectivity. These miniaturizable sensors are ideal for point-of-care devices.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
